define ORGANIC MOLECULE
those that contain CARBON (exs: carbs, lipids, proteins, nucleic acids, & adenosine triphosphate
define ISOMER
same # of atoms of each element, but different arrangements
Carbon always makes how many bonds?
4
name the 7 MAJOR FUNCTIONAL GROUPS
hydroxyl
sulfhydryl
carbonyl
carboxyl
ester
phosphate
amino
HYDROXYL
-OH
same as in bases
found in alcohols and sugars
makes organic molecules water-soluble
polar and hydrophilic
SULFHYDRYL
-SH
like hydroxyl, but sulfur+hydgrogen
important in protein structure
polar and hydrophilic
ESTER
formed from reaction of acid and alcohol
found in fats, oils, also nerve chemical acetylcholine
COOC
CARBOXYL
organic acid (H+ easily donated)
also written -COOH
usually charged (i.e. -COO-) at cellular pH
more oxygen present, more water soluble
hydrophilic
AMINO
acts as base
binds H+ to form -NH3+ at cellular pH
amino + carboxyl in same molecule is an amino acid
amine-anything with Nitrogen
NHH
PHOSPHATE
found in energy-storing molecules and in nucleic acids (DNA, RNA)
hydrophilic
ex: ATP (adenosine triphosphate)
PO42-
CARBONYL
carbon and oxygen in double covalent bond
found in KETONES, formed when fats are broken down
water-loving groups (hydrophilic)
polar
CO
arrangements that are commonly found in carbon-containing molecules
FUNCTIONAL GROUPS
what are the 4 main kinds of biological molecules?
carbohydrates (sugars)
lipids (fats)
proteins
nucleic acids
define and explain CARBOHYDRATES
5 or 6 carbons
"watered carbon"
always 1 carbon + 1 water (H2O)
formula CxH2xOx (ex: C6H12O6)
form ring structures by linking carbons 1 and 5 through an O
ratio for carbons-hydrongens-oxygen 1:2:1
carbs ALWAYS have same # of carbons as water molecules
define and explain LIPIDS (FATS)
carbon, hydrogen, and oxygen (more carbon than oxygen)
different proportions than carbs- less O2
(more O2- increased solubility)
hydrophobic or non-polar- tend to repel water
used to build cell membranes
PROTEINS
(made up of amino acids)
amino acids always contain carbon, hydrogen, oxygen, and nitrogen
some amino acids include sulfur
NUCLEIC ACIDS
sugar "backbone" plus nitrogenous base
nitrogenous base with one or two rings
one-ring pyrimidines (uracil, thymine, cytosine)
two-ring purines (adenine, guanine)
deoxyribose backbone: DNA
ribose backbone: RNA
*your body makes DNA & RNA
define MONOMER and give them for each of the 4 main kinds of biological molecules
single units
carbohydrate- monosaccharide (glucose)
protein- amino acid (there are 20)
nucleic acid- nucleotide
lipid- fatty acid
define POLYMER and give them for each of the 4 main kinds of biological molecules
result when monomers are bound together in a string or branched structure
carbohydrate- polysaccharides
protein- polypeptides (after 30, proteins)
nucleic acid- DNA & RNA
lipid- triglycerides
define DEHYDRATION SYNTHESIS
"building" (taking water out and making something)
combo of 2 monosaccharides with formation of water molecule
ALL 4 main kinds of biological molecules are dependent on fromation of bonds by dehydration synthesis
(CARBOHYDRATES) define PENTOSE
5 Carbons
ribose
deoxyribose
(CARBOHYDRATES) define HEXOSE
6 Carbons
glucose
fructose
galactose
what are the formulas for the disaccharides?
glucose+glucose=maltose
glucose+galactose=lactose
glucose+fructose=sucrose
what are your MONOSACCHARIDES?
glucose
fructose
galactose
deoxyribose
ribose
DISACCHARIDES are...?
combo of 2 monosaccharides by dehydration synthesis- formed when 2 hexoses combine
formed when the -OH of one sugar finds the -H of another and combines to form a water molecule (HOH)
sucrose (table sugar)- glucose+fructose
lactose (milk sugar)- glucose+galactose
maltose- glucose+glucose
POLYSACCHARIDES are...?
multiple monosaccharides combined by dehyration synthesis
exs: ABO blood group markers: glycogen, starch, and cellulose
explain the role of PENTOSE SUGARS in forming nucleic acids
hexoses are a food source, pentoses are NOT, rather they are essential for structure of nucleic acids (RNA & DNA)
ribose and deoxyribose differ because deoxyribose has 1 less oxygen(that's what gives it its name)
ribose-backbone for RNA
deoxyribose-backbone for DNA
what are the 3 major HEXOSES and describe them?
glucose- (MOST IMPORTANT!) human cells prefer it as an energy source, is the only significant component of "blood sugar"
fructose- found in fruit and honey"fruit sugar" (high fructose corn syrup(HFCS) is glucose converted to fructose by enzymes)
galactose- found in dairy products and sugar beets
they are all found in significant quanitities in the human diet
define HYDROLYSIS
"water breaking"
opposite of dehydration synthesis, a water molecule is added to sucrose as it is broken into glucose and fructose (what happens inside our cells as table sugar is metabolized in the diet)
hydrolysis of scrose is catalyzed by an enzyme invertase (aka sucrase)
name the carbohydrate polymers found in plants and describe each
polysaccharides(polymers of carbs) long strings of monomers, may be branched or unbranched
cellulose- cell walls, NOT digestible (fiber)
starch- storage form of glucose(major source of carbs in diet) CAN be digested
name the carb ploymer found in humans and describe
polysaccharide in humans- GLYCOGEN
major storage form of glucose in humans
mostly in the liver and MUSCLE (mostly)
easily broken down (hydrolysis) to glucose(used to power cells(carb loading))
give one example of a polysaccharide found as a surface marker (for blood ABO)
GLYCOLIPID- combo of lipids(phospholipids) and sugar(carbs) groups
these sugars mark specific cell types
process: polysaccharide is attached to a lipid molecule (one monosaccharide involved is galactose)
compare and contrast roles of mono, di, and polysaccharides in human biology
(poly) CARBS- most important constituent of human diet- can be used for energy under AEROBIC and ANAEROBIC conditions
(poly) starch from plants-important carb source
cellulose- "fiber" in diet
mono and disaccharides are used as sweeteners
show dehydration synthesis and hydrolysis reaction
--> dehydration synthesis (building)
<-- hydrolysis (breaking)
synthesis of triglycerides (polymers for lipids)
fatty acids added to glycerol backbone by dehydration synthesis
triglycerides are held together because of Ester linkage
what are the 6 types/forms of lipids in the body?
fatty acids
triglycerides
phospholipids
steroids
eicosanoids
other lipids (fat soluble vitamins & lipoproteins)
explain POLAR vs NON-POLAR
polar- charged (+ or - ions) hydrophilic (likes water)
non-polar- uncharged (no ions) hydrophobic (hates water)
define and describe FATTY ACIDS
building blocks of lipids
monomers of lipids
each ends in a carboxyl(-COOH) group, which is where the "ACID" part comes in
there are saturated and unsaturated (poly and mono) fatty acids
define SATURATED fatty acids
"filled" with hydrogen
all the carbons are filled, or saturated, with hydrogen atoms
in the zigzag pattern of carbon backbone- its straight
solid @ room temp (butter)
define UNSATURATED fatty acids
"missing" hydrogens
when double bonds form between carbon atoms, fewer hydrogens can bond to carbons
the double bond puts a "kink" in the regular zigzag patter of carbon backbone
monounsaturated= 1 double bond=1 kink
polyunsaturated= many double bonds= many kinks (liquid @ room temp-olive oil)
define and describe TRIGLYCERIDES
3 fatty acid chains added to a molecule of glycerol to form triglycerides
dont need to be same size or saturation, can be any combo
GLYCEROL backbone
define and describe PHOSPHOLIPIDS
formed when 2 fatty acid tails (carbon & hydrogen ONLY) are joined by a glycerol molecule to a phosphate-containing "head group"
cell membranes are made up of phospholipids
head- polar, hydrophilic- choline
neck- polar, hydrophilic- phosphate
glycerol- backbone
tails- non-polar, hydrophobic- fatty acid (carbon & hydrogen ONLY) molecules
define and explain AMPHIPATHIC
associated with phospholipids
has polar "head" & non-polar "tails"
forms lipid bilayers (oreo cookie) found in cell membranes
the tails associate (face) with eachother and the heads associate with water
define and explain STEROIDS
CHOLESTEROL is the basis for this class of molecules (we CAN'T live without it)
exs: vitamin D, estradiol(type of estrogen), testosterone, and cortisol
define and explain EICOSANOIDS
PROSTAGLANDINS & LEUKOTRIENES- "local hormones"- affects sight of damage right there
prostaglandins & leukotrienes derive from ARACHIDONIC ACID
they are key chemicals in immune defense and inflammation
leukotrienes contribute to inflammation associated with asthma
prostaglandins contribute to pain/inflammation associated with immune response
define and explain FAT-SOLUBLE VITAMINS
ADEK
vitamin A is synthesized from carotenes- important in vision (signs-poor teeth & gums, night blindness)
vitamin D is important in bone formation, cholesterol derivative (sign-rickets (low blood calcium, soft bones, distorted skeleton))
vitamin E is a protective molecule (skin protection)
vitamin K is important in blood clotting (sign-slow blood clotting)
define and explain LIPOPROTEIN
lipid carriers in blood
differ in density, have different effects on health
lipids are less dense than water (oil floats)
VLDL- high lipid content(low protein) Very Low Density Lipoprotein- most damaging form of lipoprotein (BRINGS fats to heart)
LDL- intermediate lipid content- Low-Density Lipoprotein
HDL- highest protein cotent, lowest lipid content, its believed to exert protective effect, its increased by exercise- High Density Lipoprotein (best one, carries fats AWAY from heart)
name the 2 FUNCTIONAL GROUPS on AMINO ACIDS and explain amino acids a little bit
2 carbons and a nitrogen form backbone (middle)
amino group- nitrogen is bonded to 2 hydrogens (on side)
carboxyl group- carbon, 2 oxygen, hydrogen (other side)
(side chain) R & H
AMINO GROUP-NH2 when uncharged, NH3 + when charged (ionized)
CARBOXYL GROUP- -COOH when uncharged, -COO- when charged (ionized)
(R GROUPS- placeholders)
all of that together, with dehydration synthesis form peptide bonds (remember the drawing of amino and carboxyl groups and then removing H2O (dehydration synthesis) to form a peptide bond) the result from that is called a dipeptide and more than 2 amino acids is called a polypeptide
enzymes, called peptidases or proteases break peptide bonds by hydrolysis
20 AMINO ACIDS- what you NEED to know
differ in their R groups & have different chemical properties:
polar(hydrophilic) vs non-polar(hydrophobic)
acidic vs basic
define DISULFIDE BONDS
formed from Cysteine, with its -R group containing a sulfhydryl
help hold proteins together
ONLY AMINO ACID WITH A SULFYHYDROL GROUP
simplest amino acid
GLYCINE
makes bend in amino acid chain (folds back into itself)
PROLINE
purple box
CLOWNS- oddballs (unique)
green box
GAYS- polar, charged
acids (left) & bases (right)
blue box
BOYS- polar, uncharged- don't share equally
in the thyroid, Iodine is added to what? to make thyroid hormones
TYROSINE
pinkish box
GIRLS- non-polar, share equally
describe protein PRIMARY STRUCTURE and what are the 2 primary sequences?
start of a protein structure
(the order in which amino acids are strung together) sequence of amino acids forming its polypeptide chains
2 primary sequences: 1-ELVIS(glutamic acid-leucine-valine-isoleucine-serine) 2-ferret beta-actin
which amino acid is always first in every protein?
Methionine
describe protein SECONDARY STRUCTURE and give examples
form a helices(coil)-ex hair(keratin)
form pleated sheats(fold)-ex proteins in nature, like spider silk- H bonds and dotted lines
amino acids that DON'T have secondary structure are THREADS
describe protein TERTIARY STRUCTURE and examples
how helices or sheets are arranged in 3D
types of atomic ineractions leading to tertiary structure: 1-ionic bonds 2-hydrophobic interactions 3-Van der Waals interactions
4-disulfide bridges 5-hydrogen bonds
what are the four levels of STRUCTURE?
primary
secondary
tertiary
quaternary
describe protein QUATERNARY and examples
2 or more identical subunits
*if a change occurs in primary, it will lead to changes in ALL of them
define DENATURATION
when we disrupt the interactions between bonds, and unfold a protein, leaving the primary structure intact
*it destroys secondary, tertiary, & quaternary structures
what are the 6 types of proteins & their functions?
STRUCTURAL-form structural framework of various parts of the body (collagen, keratin)
REGULATORY-funtion as hormones that regulate various physiological processes: control growth and development; as neurotransmitters, mediate response of the nervous system (insulin)
CONTRACTILE-allow shortening of muscle cells, which produces movement (myosin, actin)
IMMUNOLOGICAL-aid responses that protect body against foreign substances and invading pathogens (antibodies, interleukins)
TRANSPORT-carry vital substances throughout the body (hemoglobin)
CATALYTIC-act as enzymes that regulate biochemical reactions (salivary amylase, sucrase, & ATPase)
define an ENZYME
proteins that speed up (catalyze) chemical reactions
proteins that are needed in ALL cells
they lower the amount of energy needed to start the reaction, but not the overall energy profile
define ACTIVATION ENERGY
energy needed to make alignment happen
explain ENZYMES ARE CATALYSTS
CATALYSTS make reactions go faster, but are NOT CONSUMED
the substance undergoing the reaction= SUBSTRATE (TARGET)
enzymes are specific (like puzzle pieces)
what are the 3 things about enzymes?
1-speed up
2-specific
3-don't consume (reusable)
what are the bases and sugars in DNA & RNA?
DNA- bases: Adenine, Cytosine, Guanine, & Thymine
sugar-deoxyribose
RNA-bases: Adenine, Cytosine, Guanine, & Uracil
sugar-ribose
what are the 3 units/parts of NUCLEOTIDES
a base
a sugar
a phosphate group
what are the PYRIMIDINES?
URACIL, CYTOSINE, & THYMINE (CUT THE U)
what are the PURINES?
GUANINE & ADENINE
name the key structural features of DNA
consists of 2 twisted strands (double helix)
used to transmit genetic information
backbone- sugar(deoxyribose) + phosphate
always in those pairs:
(3 hydrogen bonds)
cytosine>(chewing gum) C-G base pair
guanine
(2 hydrogen bonds)
adenine>(apple tree) A-T base pair
thymine
hydrogen bonds between bases hold strands together
name the key structural features of RNA
unstable
used for temporary storage & manipulation of genetic info (scratchpad)
does NOT form double helices- single strand
backbone-sugar(ribose) + phosphate
cytosine
guanine
adenine
uracil
explain biological role of ATP (adenosine triphosphate) and how it's used to produce cellular energy
energy currency
in a subcellular compartment (mitochondrion), oxygen & glucose are converted cellular energy, which is then stored as high-energy phosphate bonds in a molecule(ATP)
what are the 5 forms of CELLULAR ENERGY?
ELECTRICAL- nerve impulses
HEAT- cellular chemical reactions
MECHANICAL- muscle contractions
POTENTIAL(chemical)- energy-rich covalent bonds
RADIANT- photosynthesis in plants
define and explain WATER SOLUBLE VITAMINS
VITAMIN C- collagen synthesis in connective tissues (signs scurvy (bleeding gums, loose teeth, swollen joints; slow wound healing; weight loss))
VITAMIN B
explain vitamins as cofactors in enzymatic reactions
need vitamins to assist or work with enzymes to facilitate better work
give element, function, & human deficiency signs of MINERALS
FLUORINE- structure of teeth/bones, effect on cells which build or break down bone; inhibits microorganisms which degrade teeth(signs-increased incidence of dental caries; osteoporosis)
CHROMIUM- efficient use of insulin(signs-relative insulin resistance; impaired glucose tolerance; elevated serum lipids)
IRON- oxygen & electron transport; heme group of hemoglobin(signs-anemia)
IODINE- constituent of thyroid hormones(signs-goiter; depression of thyroid function; (if congenital) cretinism)