Card Set Information
The order or reagent application in the gram staining procedure
crystal violet,iodine(mordant), alchol, safranin
A nanometer is equall to?
what procedure kills organisms and ensures adherence to the slide?
Tuberculosis and leprosy can be diagnosed using?
the acid fast stain
___ is used to examine specimens that are either invisible with an ordinary light microscope or can not be successfully stained by standard staining methods?
a darkfield microscope
a negative stain does not color a cell because?
the acidic dye's negative ions are repelled by the negatively charged bacterial surface.
The ability to absorb short wavelengths of light and give off light at a longer wavelength is?
What type of microscope is used to examine internal structures of organisms?
phase contrast microscope
Match the reagent with the function?
Crystal violet > Mordant
Iodine Primary >stain
Sarfranin > Decolorizer
Crystal violet>>>>Primary stain
Which of the following is mismatched?
Microscope that uses a metal-and-diamond probe and proviedes 3D images of biological specimens in nearly atimic detail?
Atomic Force Microscope
What is the purpose of the decolorizer in the gram stain?
it removes the primary stain from some organisms but not all, thus helping to differentiate between different types of organisms.
Which stains are used to stain endospores?
malachite green and sarfranin
What type of electron microscope is useful for studying surface structures of cells and viruses?
What stain can provide evidence of an organisms virulence?
Bacteria stain with basic dyes because the are slightly negatively charged at PH7
match correct descriptions.
Simple Stain> reacts different with different bacteria
Acid Fast stain> prepares colorless bacteria aganist col.
Negative>single basic dye highlights entire organism
Diffrential stain> binds strongly bacteria have waxy layer
Simple stain- a single basic dye that highlights the entire organism
Diffrential stain- reacts diffrently with different bacteria
Acid fast stain- binds strongly only to bacteria that have a waxy layer in their cell walls
Negative- prepares colorless bacteria aganist a colored background
List several ways in which microbes affect our lives.
balancing chemical in environment
List the 3 domains.