test 1

Card Set Information

test 1
2011-09-13 20:20:06

study guide
Show Answers:

  1. The order or reagent application in the gram staining procedure
    crystal violet,iodine(mordant), alchol, safranin
  2. A nanometer is equall to?
  3. what procedure kills organisms and ensures adherence to the slide?
  4. Tuberculosis and leprosy can be diagnosed using?
    the acid fast stain
  5. ___ is used to examine specimens that are either invisible with an ordinary light microscope or can not be successfully stained by standard staining methods?
    a darkfield microscope
  6. a negative stain does not color a cell because?
    the acidic dye's negative ions are repelled by the negatively charged bacterial surface.
  7. The ability to absorb short wavelengths of light and give off light at a longer wavelength is?
  8. What type of microscope is used to examine internal structures of organisms?
    phase contrast microscope
  9. Match the reagent with the function?
    Crystal violet > Mordant
    Iodine Primary >stain
    Alchol >Counterstain
    Sarfranin > Decolorizer
    • Crystal violet>>>>Primary stain
    • Iodine>>>>>>>> Mordant
    • Alchol>>>>>>>>Decolorizer
    • Sarfranin>>>>>>Counterstain
  10. Which of the following is mismatched?
    Schaeffer-Fulton-Flagella stain
  11. Microscope that uses a metal-and-diamond probe and proviedes 3D images of biological specimens in nearly atimic detail?
    Atomic Force Microscope
  12. What is the purpose of the decolorizer in the gram stain?
    it removes the primary stain from some organisms but not all, thus helping to differentiate between different types of organisms.
  13. Which stains are used to stain endospores?
    malachite green and sarfranin
  14. What type of electron microscope is useful for studying surface structures of cells and viruses?
  15. What stain can provide evidence of an organisms virulence?
    capsule stain
  16. Bacteria stain with basic dyes because the are slightly negatively charged at PH7
  17. match correct descriptions.
    Simple Stain> reacts different with different bacteria
    Acid Fast stain> prepares colorless bacteria aganist col.
    Negative>single basic dye highlights entire organism
    Diffrential stain> binds strongly bacteria have waxy layer
    • Simple stain- a single basic dye that highlights the entire organism
    • Diffrential stain- reacts diffrently with different bacteria
    • Acid fast stain- binds strongly only to bacteria that have a waxy layer in their cell walls
    • Negative- prepares colorless bacteria aganist a colored background
  18. List several ways in which microbes affect our lives.
    • Disease
    • photosynthesis
    • digestion
    • balancing chemical in environment
  19. List the 3 domains.
    • archae
    • bacteria
    • eukarya
  20. bacteria