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BIOTECH LAB EXAM 1 CHPTRS 1 & 2
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MOST OF THE LABS ARE BASED ON WHAT?
MICROCHEMICAL PROTOCALS
1 mL = HOW MUCH OF A LITER?
0.001 L
T OR F?
1,000mL = 1L
True
False
TRUE
1uL = HOW MUCH OF A LITER?
0.000001 L
(5 PLACES)
T OR F ?
1,000,000 uL = 1L
True
False
TRUE
FLAMING THE RIM OF A TUBE WARMS THE AIR AROUND THE MOUTH CREATING A --------- ------- CURRENT.
OUTWARD CONVECTION
WHAT IS USUALLY THE FIRST STEP IN GENETIC MANITIPULATION OF MICRORGANISMS?
STREAKING CELLS TO OBTAIN CFU.
T OR F?
USING CELLS DEVERIED FROM A SINGLE COLONY MINIMIZES THE CHANCE THE CELL MASS HAS BEEN CONTAMINATED.
True
False
TRUE
IF A PLASMID CONTAINS pAMP IT WILL PRODUCE AN ----- THAT -------THE AMPICILLIN.
ENZYMES
DESTROYS
WHEN GROWING E.COLI STRAINS THE CONTAIN pAMP PLASMID, WHAT SHOULD THE BROTH CONTAIN?
LB BROTH SHOULD HAVE AMPICILLIN ADDED.
WHAT DOES THE "LB" IN LB BROTH STAND FOR?
LURIA-BERTANI
T OR F ?
AMPICILLIN IS VERY STABLE IN AGAR PLATES?
True
False
TRUE
WHEN IN DOUBT WHAT TEMPERATURE WOULD YOU CULTURE E.COLI?
BELOW 37C.
NAME 2 K-12 DERIVED E-COLI STRAINS AND WHY THEY POSE NO HARM TO HUMANS.
MM294
JM101
LACK "O ANTIGEN" RESPONSIBLE FOR VIRULENCE.
T OR F?
WHEN PLATING, FLAME & COOL BEFORE EACH STREAK?
True
False
TRUE
NAME 2 CLASSES OF ANTIBIOTICS
BACTERIOSTATS-->PREVENT GROWTH.
BACTERIOCIOES-->CELL KILLER.
AMPICILLIN AND KANAMYCIN ARE BOTH WHAT TYPE OF ANTIBIOTIC?
BACTERIOCIDES...CELL KILLERS
AMPICILLIN IS A DERIVATIVE OF WHAT?
PENICILLIN
HOW DOES AMPICILLIN FUNCTION?
BLOCKS SYNTHESIS OF PEPTIDOGLYCAN LAYER THE LIES BETWEEN E.COLI INNER AND OUTER MEMBRANES.
T OR F?
AMPICILLIN DOES NOT EFFECT EXISTING CELLS BUT KILLS DIVIDING CELLS AS THEY SYNTHESIZE NEW PEPTIDOGLYCAN.
True
False
TRUE
HOW DOES KANAMYCIN FUNCTION?
BLOCKS PROEIN SYNTHESIS BY COLVALENTLY MODIFYING THE BACTERIAL RIBOSOME.
T OR F?
KANAMYCIN KILLS BOTH DIVIDING AND QUIESCENT CELLS?
True
False
TRUE
THE AMPICILLIN-RESISTANT GENE CARRIED BY THE PLASMID pAMP PRODUCES A PROTEIN CALLED WHAT?
B-LACTAMASE
THE KANAMYCIN-RESISTANT GENE OF THE PLASMID kAMP PRODUCES WHAT?
THE ENZYME KANAMYCIN PHOSPHOTRANSFERASE.
(PREVENTS KANAMYCIN INTERACTION WITH RIBOSOME.)
THE B-LACTAM RING OF AMPICILLIN STRUCTURALLY MINICS THE ----- BRIDGES BETWEEN THE TETRAPEPTIDE CHAINS AND IRREVERSIBLY BINDS THE -------.
PEPTIDE
TRANSPEPTIDASE
WHAT AMINO ACID IS INVOLVED IN AMPICILLIN?
ALA
TRANSFORMED CELL +pAMP IN LB BROTH:
NEGATIVE OR POSITIVE CONTROL?
POSITIVE CONTROL
WILD-TYPE CELL (-pAMP) BEING PLATED ON LB/AMP PLATES IS A NEGATIVE/POSITIVE CONTROL?
NEGATIVE CONTROL
KANAMYCIN POISONS PROTEIN SYNTHESIS BY IRREVERSIBLY BINDING THE -----SUBUNIT OF THE -------.
30S
RIBSOME
A 15mL CULTURE TUBE IS NOT THE BEST CHOICE FOR E.COLI GROWTH. A 50mL IS BETTER. WHY?
GREATER SURFACE AREA FOR AERATION.
WHY IS IT IDEAL TO PROVIDE CONTINUOUS SHAKING FOR A SUSPENSION CULTURE? GIVE 2 REASONS.
SHAKING INCREASE AERATION.
PURIFYING PLASMID.
FACILITATES EXCHANGE OF NUTRIENTS.
FLUSHES AWAY WASTE PRODUCTS.
WHAT GROWTH PHASE IS REACHED BY SUSPENSION OF E.COLI FOLLOWING OVERNIGHT SHAKING AT 37C?
STATIONARY.
APPROXIMATELY HOW MANY E.COLI CELLS ARE IN A 5mL SUSPENSION CULTURE AT STATIONARY PHASE?
10^9 CELLS/mL
NAME THE FOUR STAGES OF THE GROWTH CURVE.
LAG
LOG
STATIONARY
DEATH
HOW LONG IS THE LAG PHASE?
30MNS.
WHEN DONE BY PROCEDURE IN LAB E.COLI STRAINS MM294 AND JM101 REACH MID-LOG PHASE AFTER ----- TO ----- MINUTES OF INCUBATION.
90-110 MINUTES
T OR F?
A CULTURE INOCULATED INTO LB BROTH PREWARMED TO 37C WILL REACH MID-LOG PAHSE QUICKER THAN ONE BEGUN AT ROOM TEMP.
True
False
TRUE.
Author
shockwave
ID
104150
Card Set
BIOTECH LAB EXAM 1 CHPTRS 1 & 2
Description
BIOTECH LAB EXAM 1 CHPTRS 1 & 2
Updated
9/25/2011, 6:41:22 PM
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