BIOTECH LAB EXAM 1 CHPTRS 1 & 2

MOST OF THE LABS ARE BASED ON WHAT?

1 mL = HOW MUCH OF A LITER?

T OR F?
1,000mL = 1L
True False

1uL = HOW MUCH OF A LITER?

T OR F ?
1,000,000 uL = 1L
True False

FLAMING THE RIM OF A TUBE WARMS THE AIR AROUND THE MOUTH CREATING A --------- ------- CURRENT.

WHAT IS USUALLY THE FIRST STEP IN GENETIC MANITIPULATION OF MICRORGANISMS?

T OR F?
USING CELLS DEVERIED FROM A SINGLE COLONY MINIMIZES THE CHANCE THE CELL MASS HAS BEEN CONTAMINATED.
True False

IF A PLASMID CONTAINS pAMP IT WILL PRODUCE AN ----- THAT -------THE AMPICILLIN.

WHEN GROWING E.COLI STRAINS THE CONTAIN pAMP PLASMID, WHAT SHOULD THE BROTH CONTAIN?

WHAT DOES THE "LB" IN LB BROTH STAND FOR?

T OR F ?
AMPICILLIN IS VERY STABLE IN AGAR PLATES?
True False

WHEN IN DOUBT WHAT TEMPERATURE WOULD YOU CULTURE E.COLI?

NAME 2 K-12 DERIVED E-COLI STRAINS AND WHY THEY POSE NO HARM TO HUMANS.

T OR F?
WHEN PLATING, FLAME & COOL BEFORE EACH STREAK?
True False

NAME 2 CLASSES OF ANTIBIOTICS

AMPICILLIN AND KANAMYCIN ARE BOTH WHAT TYPE OF ANTIBIOTIC?

AMPICILLIN IS A DERIVATIVE OF WHAT?

HOW DOES AMPICILLIN FUNCTION?

T OR F?
AMPICILLIN DOES NOT EFFECT EXISTING CELLS BUT KILLS DIVIDING CELLS AS THEY SYNTHESIZE NEW PEPTIDOGLYCAN.
True False

HOW DOES KANAMYCIN FUNCTION?

T OR F?
KANAMYCIN KILLS BOTH DIVIDING AND QUIESCENT CELLS?
True False

THE AMPICILLIN-RESISTANT GENE CARRIED BY THE PLASMID pAMP PRODUCES A PROTEIN CALLED WHAT?

THE KANAMYCIN-RESISTANT GENE OF THE PLASMID kAMP PRODUCES WHAT?

THE B-LACTAM RING OF AMPICILLIN STRUCTURALLY MINICS THE ----- BRIDGES BETWEEN THE TETRAPEPTIDE CHAINS AND IRREVERSIBLY BINDS THE -------.

WHAT AMINO ACID IS INVOLVED IN AMPICILLIN?

TRANSFORMED CELL +pAMP IN LB BROTH:
NEGATIVE OR POSITIVE CONTROL?

WILD-TYPE CELL (-pAMP) BEING PLATED ON LB/AMP PLATES IS A NEGATIVE/POSITIVE CONTROL?

KANAMYCIN POISONS PROTEIN SYNTHESIS BY IRREVERSIBLY BINDING THE -----SUBUNIT OF THE -------.

A 15mL CULTURE TUBE IS NOT THE BEST CHOICE FOR E.COLI GROWTH. A 50mL IS BETTER. WHY?

WHY IS IT IDEAL TO PROVIDE CONTINUOUS SHAKING FOR A SUSPENSION CULTURE? GIVE 2 REASONS.

WHAT GROWTH PHASE IS REACHED BY SUSPENSION OF E.COLI FOLLOWING OVERNIGHT SHAKING AT 37C?

APPROXIMATELY HOW MANY E.COLI CELLS ARE IN A 5mL SUSPENSION CULTURE AT STATIONARY PHASE?

NAME THE FOUR STAGES OF THE GROWTH CURVE.

HOW LONG IS THE LAG PHASE?

WHEN DONE BY PROCEDURE IN LAB E.COLI STRAINS MM294 AND JM101 REACH MID-LOG PHASE AFTER ----- TO ----- MINUTES OF INCUBATION.

T OR F?
A CULTURE INOCULATED INTO LB BROTH PREWARMED TO 37C WILL REACH MID-LOG PAHSE QUICKER THAN ONE BEGUN AT ROOM TEMP.
True False