BIOTECH LAB EXAM 1 CHPTRS 1 & 2

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shockwave
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104150
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BIOTECH LAB EXAM 1 CHPTRS 1 & 2
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2011-09-25 14:41:22
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BIOTECH LAB EXAM CHPTRS
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BIOTECH LAB EXAM 1 CHPTRS 1 & 2
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  1. MOST OF THE LABS ARE BASED ON WHAT?
    MICROCHEMICAL PROTOCALS
  2. 1 mL = HOW MUCH OF A LITER?
    0.001 L
  3. T OR F?
    1,000mL = 1L
    TRUE
  4. 1uL = HOW MUCH OF A LITER?
    • 0.000001 L
    • (5 PLACES)
  5. T OR F ?
    1,000,000 uL = 1L
    TRUE
  6. FLAMING THE RIM OF A TUBE WARMS THE AIR AROUND THE MOUTH CREATING A --------- ------- CURRENT.
    OUTWARD CONVECTION
  7. WHAT IS USUALLY THE FIRST STEP IN GENETIC MANITIPULATION OF MICRORGANISMS?
    STREAKING CELLS TO OBTAIN CFU.
  8. T OR F?
    USING CELLS DEVERIED FROM A SINGLE COLONY MINIMIZES THE CHANCE THE CELL MASS HAS BEEN CONTAMINATED.
    TRUE
  9. IF A PLASMID CONTAINS pAMP IT WILL PRODUCE AN ----- THAT -------THE AMPICILLIN.
    • ENZYMES
    • DESTROYS
  10. WHEN GROWING E.COLI STRAINS THE CONTAIN pAMP PLASMID, WHAT SHOULD THE BROTH CONTAIN?
    LB BROTH SHOULD HAVE AMPICILLIN ADDED.
  11. WHAT DOES THE "LB" IN LB BROTH STAND FOR?
    LURIA-BERTANI
  12. T OR F ?
    AMPICILLIN IS VERY STABLE IN AGAR PLATES?
    TRUE
  13. WHEN IN DOUBT WHAT TEMPERATURE WOULD YOU CULTURE E.COLI?
    BELOW 37C.
  14. NAME 2 K-12 DERIVED E-COLI STRAINS AND WHY THEY POSE NO HARM TO HUMANS.
    • MM294
    • JM101
    • LACK "O ANTIGEN" RESPONSIBLE FOR VIRULENCE.
  15. T OR F?
    WHEN PLATING, FLAME & COOL BEFORE EACH STREAK?
    TRUE
  16. NAME 2 CLASSES OF ANTIBIOTICS
    • BACTERIOSTATS-->PREVENT GROWTH.
    • BACTERIOCIOES-->CELL KILLER.
  17. AMPICILLIN AND KANAMYCIN ARE BOTH WHAT TYPE OF ANTIBIOTIC?
    BACTERIOCIDES...CELL KILLERS
  18. AMPICILLIN IS A DERIVATIVE OF WHAT?
    PENICILLIN
  19. HOW DOES AMPICILLIN FUNCTION?
    BLOCKS SYNTHESIS OF PEPTIDOGLYCAN LAYER THE LIES BETWEEN E.COLI INNER AND OUTER MEMBRANES.
  20. T OR F?
    AMPICILLIN DOES NOT EFFECT EXISTING CELLS BUT KILLS DIVIDING CELLS AS THEY SYNTHESIZE NEW PEPTIDOGLYCAN.
    TRUE
  21. HOW DOES KANAMYCIN FUNCTION?
    BLOCKS PROEIN SYNTHESIS BY COLVALENTLY MODIFYING THE BACTERIAL RIBOSOME.
  22. T OR F?
    KANAMYCIN KILLS BOTH DIVIDING AND QUIESCENT CELLS?
    TRUE
  23. THE AMPICILLIN-RESISTANT GENE CARRIED BY THE PLASMID pAMP PRODUCES A PROTEIN CALLED WHAT?
    B-LACTAMASE
  24. THE KANAMYCIN-RESISTANT GENE OF THE PLASMID kAMP PRODUCES WHAT?
    • THE ENZYME KANAMYCIN PHOSPHOTRANSFERASE.
    • (PREVENTS KANAMYCIN INTERACTION WITH RIBOSOME.)
  25. THE B-LACTAM RING OF AMPICILLIN STRUCTURALLY MINICS THE ----- BRIDGES BETWEEN THE TETRAPEPTIDE CHAINS AND IRREVERSIBLY BINDS THE -------.
    • PEPTIDE
    • TRANSPEPTIDASE
  26. WHAT AMINO ACID IS INVOLVED IN AMPICILLIN?
    ALA
  27. TRANSFORMED CELL +pAMP IN LB BROTH:
    NEGATIVE OR POSITIVE CONTROL?
    POSITIVE CONTROL
  28. WILD-TYPE CELL (-pAMP) BEING PLATED ON LB/AMP PLATES IS A NEGATIVE/POSITIVE CONTROL?
    NEGATIVE CONTROL
  29. KANAMYCIN POISONS PROTEIN SYNTHESIS BY IRREVERSIBLY BINDING THE -----SUBUNIT OF THE -------.
    • 30S
    • RIBSOME
  30. A 15mL CULTURE TUBE IS NOT THE BEST CHOICE FOR E.COLI GROWTH. A 50mL IS BETTER. WHY?
    GREATER SURFACE AREA FOR AERATION.
  31. WHY IS IT IDEAL TO PROVIDE CONTINUOUS SHAKING FOR A SUSPENSION CULTURE? GIVE 2 REASONS.
    • SHAKING INCREASE AERATION.
    • PURIFYING PLASMID.
    • FACILITATES EXCHANGE OF NUTRIENTS.
    • FLUSHES AWAY WASTE PRODUCTS.
  32. WHAT GROWTH PHASE IS REACHED BY SUSPENSION OF E.COLI FOLLOWING OVERNIGHT SHAKING AT 37C?
    STATIONARY.
  33. APPROXIMATELY HOW MANY E.COLI CELLS ARE IN A 5mL SUSPENSION CULTURE AT STATIONARY PHASE?
    10^9 CELLS/mL
  34. NAME THE FOUR STAGES OF THE GROWTH CURVE.
    • LAG
    • LOG
    • STATIONARY
    • DEATH
  35. HOW LONG IS THE LAG PHASE?
    30MNS.
  36. WHEN DONE BY PROCEDURE IN LAB E.COLI STRAINS MM294 AND JM101 REACH MID-LOG PHASE AFTER ----- TO ----- MINUTES OF INCUBATION.
    90-110 MINUTES
  37. T OR F?
    A CULTURE INOCULATED INTO LB BROTH PREWARMED TO 37C WILL REACH MID-LOG PAHSE QUICKER THAN ONE BEGUN AT ROOM TEMP.
    TRUE.

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