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MOST OF THE LABS ARE BASED ON WHAT?
MICROCHEMICAL PROTOCALS
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1 mL = HOW MUCH OF A LITER?
0.001 L
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T OR F?
1,000mL = 1L
TRUE
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1uL = HOW MUCH OF A LITER?
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T OR F ?
1,000,000 uL = 1L
TRUE
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FLAMING THE RIM OF A TUBE WARMS THE AIR AROUND THE MOUTH CREATING A --------- ------- CURRENT.
OUTWARD CONVECTION
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WHAT IS USUALLY THE FIRST STEP IN GENETIC MANITIPULATION OF MICRORGANISMS?
STREAKING CELLS TO OBTAIN CFU.
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T OR F?
USING CELLS DEVERIED FROM A SINGLE COLONY MINIMIZES THE CHANCE THE CELL MASS HAS BEEN CONTAMINATED.
TRUE
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IF A PLASMID CONTAINS pAMP IT WILL PRODUCE AN ----- THAT -------THE AMPICILLIN.
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WHEN GROWING E.COLI STRAINS THE CONTAIN pAMP PLASMID, WHAT SHOULD THE BROTH CONTAIN?
LB BROTH SHOULD HAVE AMPICILLIN ADDED.
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WHAT DOES THE "LB" IN LB BROTH STAND FOR?
LURIA-BERTANI
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T OR F ?
AMPICILLIN IS VERY STABLE IN AGAR PLATES?
TRUE
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WHEN IN DOUBT WHAT TEMPERATURE WOULD YOU CULTURE E.COLI?
BELOW 37C.
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NAME 2 K-12 DERIVED E-COLI STRAINS AND WHY THEY POSE NO HARM TO HUMANS.
- MM294
- JM101
- LACK "O ANTIGEN" RESPONSIBLE FOR VIRULENCE.
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T OR F?
WHEN PLATING, FLAME & COOL BEFORE EACH STREAK?
TRUE
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NAME 2 CLASSES OF ANTIBIOTICS
- BACTERIOSTATS-->PREVENT GROWTH.
- BACTERIOCIOES-->CELL KILLER.
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AMPICILLIN AND KANAMYCIN ARE BOTH WHAT TYPE OF ANTIBIOTIC?
BACTERIOCIDES...CELL KILLERS
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AMPICILLIN IS A DERIVATIVE OF WHAT?
PENICILLIN
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HOW DOES AMPICILLIN FUNCTION?
BLOCKS SYNTHESIS OF PEPTIDOGLYCAN LAYER THE LIES BETWEEN E.COLI INNER AND OUTER MEMBRANES.
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T OR F?
AMPICILLIN DOES NOT EFFECT EXISTING CELLS BUT KILLS DIVIDING CELLS AS THEY SYNTHESIZE NEW PEPTIDOGLYCAN.
TRUE
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HOW DOES KANAMYCIN FUNCTION?
BLOCKS PROEIN SYNTHESIS BY COLVALENTLY MODIFYING THE BACTERIAL RIBOSOME.
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T OR F?
KANAMYCIN KILLS BOTH DIVIDING AND QUIESCENT CELLS?
TRUE
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THE AMPICILLIN-RESISTANT GENE CARRIED BY THE PLASMID pAMP PRODUCES A PROTEIN CALLED WHAT?
B-LACTAMASE
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THE KANAMYCIN-RESISTANT GENE OF THE PLASMID kAMP PRODUCES WHAT?
- THE ENZYME KANAMYCIN PHOSPHOTRANSFERASE.
- (PREVENTS KANAMYCIN INTERACTION WITH RIBOSOME.)
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THE B-LACTAM RING OF AMPICILLIN STRUCTURALLY MINICS THE ----- BRIDGES BETWEEN THE TETRAPEPTIDE CHAINS AND IRREVERSIBLY BINDS THE -------.
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WHAT AMINO ACID IS INVOLVED IN AMPICILLIN?
ALA
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TRANSFORMED CELL +pAMP IN LB BROTH:
NEGATIVE OR POSITIVE CONTROL?
POSITIVE CONTROL
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WILD-TYPE CELL (-pAMP) BEING PLATED ON LB/AMP PLATES IS A NEGATIVE/POSITIVE CONTROL?
NEGATIVE CONTROL
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KANAMYCIN POISONS PROTEIN SYNTHESIS BY IRREVERSIBLY BINDING THE -----SUBUNIT OF THE -------.
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A 15mL CULTURE TUBE IS NOT THE BEST CHOICE FOR E.COLI GROWTH. A 50mL IS BETTER. WHY?
GREATER SURFACE AREA FOR AERATION.
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WHY IS IT IDEAL TO PROVIDE CONTINUOUS SHAKING FOR A SUSPENSION CULTURE? GIVE 2 REASONS.
- SHAKING INCREASE AERATION.
- PURIFYING PLASMID.
- FACILITATES EXCHANGE OF NUTRIENTS.
- FLUSHES AWAY WASTE PRODUCTS.
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WHAT GROWTH PHASE IS REACHED BY SUSPENSION OF E.COLI FOLLOWING OVERNIGHT SHAKING AT 37C?
STATIONARY.
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APPROXIMATELY HOW MANY E.COLI CELLS ARE IN A 5mL SUSPENSION CULTURE AT STATIONARY PHASE?
10^9 CELLS/mL
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NAME THE FOUR STAGES OF THE GROWTH CURVE.
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HOW LONG IS THE LAG PHASE?
30MNS.
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WHEN DONE BY PROCEDURE IN LAB E.COLI STRAINS MM294 AND JM101 REACH MID-LOG PHASE AFTER ----- TO ----- MINUTES OF INCUBATION.
90-110 MINUTES
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T OR F?
A CULTURE INOCULATED INTO LB BROTH PREWARMED TO 37C WILL REACH MID-LOG PAHSE QUICKER THAN ONE BEGUN AT ROOM TEMP.
TRUE.
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