Diversity Lab Practical

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Anonymous
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104972
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Diversity Lab Practical
Updated:
2011-09-28 17:07:40
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bio
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Bio 110 Lab Practical
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  1. what distinguishes Archae from Diplomonads?
    Diplomonads have membrane-bound nuclues, Archaea don't
  2. What do chitin and cellulose have in common?
    both are primarily composed of glucose
  3. In the electrophoresis lab, what was the independent variable?
    the pH of the buffer used to prepare the gels
  4. What is the isoelectric point of a protein?
    the pH at which the protein will not migrate in an electrical field.
  5. If the isoelectric point of a protein is higher than the pH, what happens?
    the protein will have a net positive charge
  6. What microscope is best for viewing the surface features of an insect that is 10mm in length?
    the Leica 2000 stereomicroscope
  7. Which part of the Nikon microscope should not be adjusted with the 40X objective in place?
    Course focus knob
  8. What was the purpose of the iodine in the microscopy lab?
    Stained some cell parts more intensely than others, creating contrast between the parts.
  9. In the permeability lab, what was the dependent variable?
    permeability
  10. In the permeability lab, what were the independent variables?
    Size, charge, and lipid solubility
  11. In the electrophoresis lab, what was the dependent variable?
    the distance each protein migrated in an electric field
  12. What was the measure of permeability used in lab?
    the time required to reach one-half maximum transparency after injecting the solution
  13. What is magnification?
    making things appear larger
  14. What is resolution?
    being able to distinguish 2 objects
  15. What is contrast?
    different parts of an object absorb or transmit amounts of light
  16. What do you do to calibrate a spec 20?
    put a blank slide in turn the left knob so it reads 0. then turn the right knob so it reads 100.
  17. What distinguishes prokaryotes from eukaryotes?
    they don't have a nuclues, cytoskeleton, or membrane-bound organelles.
  18. What are the different shapes for the different Bacteria?
    • Spherical- cocci
    • Rod-shaped-Bacilli
    • Spiral-Spirilla
  19. What is the different between autotrophic and heterotrophic?
    • autotrophic- feed themselves
    • heterotrophic- feed on other things
  20. What are the 3 main divisions of fungi?
    Zygomycota, Basidiomycota,and Ascomycota

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