Cytoskeleton Actin Structure

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  1. What is the width and subunit composition of Microfilament (2), Microtubule (3), and IF(4)?
    • Actin (/MreB): 7-9 nm
    • Alpha + Beta Tubulin dimer: 25 nm (hollow)
    • Lamins, Desmin, Keratin, Neurofilaments: 10 nm
  2. Specifications of Actin:
    • Highly conserved
    • Most abundant Eukaryotic protein
    • G-Actin + F-Actin forms
    • Complexed with ATP-Mg or ADP-Mg
  3. G and F- Actin sturcture
    • G-Actin: Monomer with 4 domains. domains II+IV= (-) end I+III=(+) end and a cleft for ATP on the (+) end
    • F-Actin: Homomer made of G-Actin monomers the form a left handed helix (pointed end = (-); Barbed end = (+))
  4. Define: Cc
    +/- side characteristics + [Cc]
    • Cc: the concentration of G-actin required for monomer assembly into filaments.
    • + side has a lower Cc which allows it to assemble in one direction
    • [+] = .12 uM [-] = .6 uM
  5. Different Cc allow for ______ which is the process by which a cell moves its membrane.
    Treadmilling: Cc of cell is inbetween + and - end which allows forward movement and is powered by ATP hydrolysis
  6. The 3 phases of Actin polymerization are:
    Nucleation - Elongation - Stablization
  7. Drugs that restrict actin assembly include:
    • Cytocholasin D: Bocks + end assembly
    • Latrunculin: Blocks G-actin addition
  8. Drugs that stabalize actin fimalaments:
    Phalloidin: Used as actin specific stain
  9. 2 proteins that regulate actin polymerization are:
    • Thymosin Beta: binds G-Actin 1:1 and prevents assembly
    • Profilin: Promotes ATP binding (+ end assembly) and binds to PIP2 at membrane

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Author:
p.reilly1227
ID:
106675
Filename:
Cytoskeleton Actin Structure
Updated:
2011-10-05 20:27:38
Tags:
Actin Cytoskeleton two
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Cytoskeleton Actin Structure
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