Predict the elution order (i.e. order they flow off
the column) of the following proteins from a Sephadex G100 size exclusion column:
Bovine Carbonic anhydrase 31 Kd,
BSA = 66 Kd,;
Egg albumin = 45 Kd,,
Rabbit skeletal muscle myosin = 200 Kd,.
- Rabbit skeletal muscle myosin (1st) (200kD),
- BSA (2nd), (66kD)
- Egg albumin (3rd) (45kD),
- Bovine Carbonic anhydrase(4th) (31kD).