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2011-10-11 15:10:18
Lab practical guide

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  1. Aseptic Technique: Work Area Disinfection
    Work area is treated with a disinfectant that will kill any microorganisms that may be present, but not endospores
  2. Aseptic Technique: Loops and Needles
    A loop or needle is sterilized by inserting it into a Bunsen burner flame until it is red hot, ensuring that all contaminating organisms will be incinerated. Allow the loop/needle to cool completely before picking up any inoculum, this will ensure that all viable cells are transferred.
  3. Aseptic Technique: Culture Tube Flaming and Inoculation
    The cap is removed, and the mouth of the tube is passed through the flame. If the tube is a broth tube, the loop is inserted into the tube and twisted several times to ensure the organisms on the loop are deliverd to the liquid. If the tube is an agar slant, the surface of the slant is inoculated by drawing the loop up the surface of the slant from the bottom of the slant to the top. For stab cultures, a needle is inserted into the agar medium. After the culture is inoculated the mouth if the tube is reflamed, and the tube is recapped.
  4. Aseptic Technique: Final Flame of the Looop or Needle
    After completing the innoculation the needle/loop is flamed in the bunsen burner, to destroy any organsims. Iteams should be returned to their appropriate place, never laid on the desk.
  5. Aseptic Technique: Petri Plate Inocultaions
    Loops are used to inocutlate or streak petri plates. The plate cover is raised andheld diagonally over the plate to protect the surface from any contaminationin the air. The loop containing the inoculom is then streaked gently over the surface of the agar
  6. EX. 6 Bacterial Ubiquity
    Basic Morphology and Arrangements of Bacterial Cells
  7. EX. 6 Bacterial Ubiquity
    Pouring Agar Plates
  8. EX. 6 Bacterial Ubiquity
    Physical Properties of Agar
  9. EX. 6 Bacterial Ubiquity
    Method of Labeling Plates
  10. EX. 6 Bacterial Ubiquity
    Incubation of Plates
  11. EX. 6 Bacterial Ubiquity
    Bacterial and Mold Growth Characteristics on Agar
  12. EX. 6 Bacterial Ubiquity
    Environmental and Body Sampling Techniques
  13. EX. 8 Aseptic Technique
    Transfer of Organisms From Broth to Another Broth
  14. EX. 8 Aseptic Technique
    Transferring of Organisms From Slant to Another Slant
  15. EX. 8 Aseptic Technique
    Transferring Organisms From Petri Plate to Slant
  16. EX. 8 Aseptic Technique
    Inoculation Techniques For Agar Slants and Broth
  17. EX. 10 Smear Preparation
    Degreasing Slides
  18. EX. 10 Smear Preparation
    Steps in Smear Prep.
  19. EX. 11 Simple Staining
    Negatively Charged Bacterial Cells
  20. EX. 11 Simple Staining
    Basic Dyes
  21. EX. 11 Simple Staining
    Acidic Dyes
  22. EX. 11 Simple Staining
  23. EX. 11 Simple Staining
    Escherichia coli
  24. EX. 11 Simple Staining
    Dyes Used
  25. EX. 11 Simple Staining
    Simple Staining vs. Differential Staining
  26. EX. 11 Simple Staining
    Pros and Cons of Simple Staining
  27. EX. 12 Negative Staining
    Smear Procedure/ Dye Used
  28. EX. 12 Negative Staining
    Staphylococcus aureus
  29. EX. 11 Simple Staining
    Corynebacterium diphtheriae
  30. EX. 12 Negative Staining
    Bacillus megaterium
  31. EX. 12 Negative Staining
    Pros and Cons of Negatice Staining
  32. EX. 14 Gram Staining
    Reagent Used
  33. EX. 14 Gram Staining
    Steps in Procedure
  34. EX. 14 Gram Staining
    Results of Each Step
  35. EX. 14 Gram Staining
    Escherichia coli
  36. EX. 14 Gram Staining
    Staphylococcuy aureus
  37. EX. 14 Gram Staining
    Mycobacterium smegmatis
  38. EX. 14 Gram Staining
    Bacillus megaterium
  39. EX. 14 Gram Staining
    Clinical Relevance
  40. EX. 14 Gram Staining
    Diagnostic Utility
  41. EX. 15 Spore Staining
    Componants of Endospore
  42. EX. 15 Spore Staining
    Procedure Schaeffer- Fulton
  43. EX. 15 Spore Staining
    Dorner Method
  44. EX. 15 Spore Staining
    Bacillus megaterium
  45. EX. 15 Spore Staining
    Spore Forming Bacteria
  46. EX. 16 Acid Fast Staining Zeihl-Nielson
    Cell Wall Characteristics
  47. EX. 16 Acid Fast Staining Zeihl-Nielson
    Reagents Used
  48. EX. 16 Acid Fast Staining Zeihl-Nielson
  49. EX. 16 Acid Fast Staining. Zeihl-Nielson
    Microbes Screened With This Method
  50. EX. 16 Acid Fast Staining/ Zeihl-Nielson
    Staphylococcus aureus
  51. EX. 16 Acid Fast Staining/ Zeihl-Nielson
    Mycobacterium smegmatis
  52. EX. 9 Pure Culture
    Quadrant Streaking
  53. EX. 9 Pure Culture
    Subculturing Techniques
  54. EX. 9 Pure Culture
    Eschericha coli
  55. EX. 9 Pure Culture
    Staphylococcus aureus
  56. EX. 9 Pure Culture
    Serratia marcescens
  57. EX 17 Motility
    Staphylococcus aureus
  58. EX 17 Motility
    Proteus mirabilis
  59. EX 17 Motility
    Microscopic Procedure
  60. EX 17 Motility
    Semi- solid medium- Inoculation and reading result
  61. EX 17 Motility
    Motile Microbes
  62. EX 17 Motility
    Non-Motile Microbes
  63. EX. 19 Anaerobic Culture
  64. EX. 19 Anaerobic Culture
  65. EX. 19 Anaerobic Culture
    Use of Mediums
  66. EX. 19 Anaerobic Culture
    Growth Patterns of Aerotolerance
  67. EX. 19 Anaerobic Culture
    Escherichia coli
  68. EX. 19 Anaerobic Culture
    Pseudomonas aeruginosa
  69. EX. 19 Anaerobic Culture
    Clostridium sporogenes
  70. EX. 20 Enumeration of Bacteria
    Dilutions: Calculations and Dilution Factors
  71. EX. 20 Enumeration of Bacteria
    Plates for Appropriate Counting
  72. EX. 20 Enumeration of Bacteria
    Cell Density of Original Culture- Formula
  73. EX. 20 Enumeration of Bacteria
    Meaning and Concept
  74. EX. 20 Enumeration of Bacteria
    Spec 20
  75. EX. 20 Enumeration of Bacteria
    Estimate Cell Density Using Standard Curve
  76. EX. 20 Enumeration of Bacteria
    Bacteria Used

    Escherichia coli