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Name some "Don'ts" on Lab precautions
- -Recap needles
- -Remove used needles from disposable syringes by hand
- -Bend, break, or manipulate used needles
What are the components of Blood?
- Fluid portion is 55%
Plasma contains what? and what is it used for?
Fibrinogen used to form clot(fibrin)
What are some reasons for patient isolation?
- To prevent spread of infection
- To protect immuno-compromised patient
- -normal flora of phlebotomist
- -from nosocomial pathogens
For the Blue Top, what is the formula for adjusting anticoagulant
- 100-HCT x Sample volume= Citrate
The Green top contains what?
- Lithium Heparin, prevents coagulation by
- -interacting w/ anti-thrombin III
- -inhibiting thrombin
The dilution factor of anticoagulant to blood in the sodium citrate tube is what ratio?
Name 5 types of Patient Isolation?
To compensate for the dilution of anticoagulant to blood, the platelet count must be multiplied by what number?
1.1 (addition of 10% to the intial result)
What are two methods of venipuncture?
Name 3 main venipuncture sites .
- -median cubital (site of choice, anchored, stabilized vein by tissue)
- -Basilic (tends to roll, located inner portion)
- -cephalic (located outer portion, skin little tougher)
When checking for Patient Identification what are two important steps you must do?
Check pts SSN and name
Do not allow the tourniquet to stay on longer than how many minutes?
What happens if you leave the tourniquet longer than 1 min?
- Hemoconcentration could occur
- Falsely elevates test result
What should you do if your pt experience the first sign of adverse reaction?
- D/C blood collection
- Release tourniquet & remove needle
- Get pt to a private room
Capilllary collection are used in what situatuions?
In infants <6 months old
Why would you use capillary collection on infants <6 months old?
- Dangerous to remove too much blood
- Has small volume of blood
What is the correct puncture site for neonates/infants?
True or False
You can puncture the curvature of the heel of an infant.
What is the puncture depth for capillary collection of an infant?
no more than 1.6mm
What reagents and Equipment do you need for a capillary collection?
- 70% Isopropyl alcohol
- sterile gauze
- sterile blood lancets
- capillary tubes
What are the steps to collecting capillary blood?
- Ensure puncture site is warm
- clean w/ alcohol
- puncture against grain of skin quickly and firmly
- wipe away first drop of blood
- 1ml of blood
What are some comlications you can run into when collecting capillary blood?
- -tissue juice dilution
- -platelet clumping
- (collect platelet sample first)
- -Painful procedure
- (be truthful to children)
Strict Isolation protects....
When do you use strict isolation?
- in contagious dieseases transmitted via:
- -direct contact
What is the purpose of Peripheral Smear?
- To examine cells under microscope
- Test is called WBC differential
- -Different types WBC
- -RBC morphology
- -Platelet estimate
Name some Wedge smear Reagents & Equipment?
- -Two clean glass slides
- -capillary tubes
- -EDTA blood sample
Why should you prepare a smears within _hrs?
- 2 Hours
- RBC's become macrocytic
- WBC's & platelets degenerate
What are some good qualities of a good (peripheral) smear?
- -feathered edge
- -w/ rainbow appearance
- -free of holes, ridges & waves
What are some qualities of properly Stained slides?
- Eosinophils have bright orange granules
- Basophils have dark blue-black granules
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