Microbiology Lab Quiz #2

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Author:
aji
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112358
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Microbiology Lab Quiz #2
Updated:
2011-10-26 23:46:06
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itsaji PHCC
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LAB 3 & 4
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  1. ___ test that determines if an organism is infected with the bacteria that causes tuberculosis and other illnesses
    Acid Fast Stain
  2. What is the purpose of doing an acid fast stain ?
    Detects cells capacity to retain primary stain
  3. Acid Fast stain detects the presence of __
    AFB - Acid Fast Bacilli
  4. Disease identified by acid fast stain
    • Tuberculosis
    • Leprae
    • Smegmatis
  5. Steps involved in acid fast stain
    • 1 Primary Stain - Carbofuchsin
    • 2 Decolorizing - Acid Alcohol
    • 3 Counter Stain - Methylene Blue
  6. Color of Acid fast Bacteria (+tive result)
    Red
  7. Color of Non-Acid fast Bacteria (-tive result)
    Blue
  8. Name 3 organism positive for capsule staining
    • Bacillus Anthracis
    • Streptococcus Pneumoniae
    • Klebsiella Pneumoniae
  9. What does +tive capsule stain tells
    • Ability of a cell to produce extracellular capsule.
    • 90% of them are pathogenic
  10. How does capsule contribute to its pathogenicity.
    Capsule makes them less vulnerable to phagocytosis
  11. Purpose of endospore stain
    It is used to detect the presence and location of spores in a cell
  12. Two genera identified by presence of endospores
    Bacillus & Clostridum
  13. Name 3 steps involved in Endospore stain:
    • 1 Primary Stain - Malachite Green
    • 2 Rinse with water
    • 3 Counter stain - Safranin
  14. Cells +tive for endospores will stain
    Green
  15. Cells -tive for endospores will stain
    Red
  16. Blood Agar is selective for ___ & differential for ___
    Streptococcus, Hemolyze RBC
  17. Mannitol Salt Agar is selective for ___ & differential for ___
    Staphylococcus, Ferment Mannitol
  18. Eosin Methylene Blue Agar is selective for ___ & differential for ___
    Coliform Bacteria, Ferment Lactose
  19. MacConkey Agar is selective for ___ & differential for ___
    Entrobacteriaceae,Ferment Lactose
  20. ___ Media can be used to differentiate pathogenic from non-pathogenic bacteria
    Differential Media
  21. Most selective media select for the growth of ___ & ___ organism
    Gram +tive & Gram -tive
  22. Mannitol Salt Agar (MSA) +tive color
    Phenol Red turns Yellow
  23. Mannitol Salt Agar (MSA) -tive color
    Red
  24. Eosin Methylene Blue Agar (EMB) +tive
    • Metallic Green - Non Pathogenic
    • Pink - Mild Pathogenic
    • White - Strong Pathogenic
  25. Eosin Methylene Blue Agar (EMB) -tive
    Normal or Medium Color
  26. MacConey Agar(MAC) +tive Pathogenic
    White
  27. MacConey Agar(MAC) -tive Non-Pathogenic
    Pink

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