Microbiology Quiz 3 - 1

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Author:
aji
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115846
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Microbiology Quiz 3 - 1
Updated:
2011-11-10 08:08:50
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itsaji PHCC
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Lab Tests
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  1. Oxidase Test
    • The oxidase test identifies organisms that produce the enzyme cytochrome oxidase.
    • If the test organism produces cytochrome oxidase, the oxidase reagent will turn blue or purple within 15 seconds.
    • E.coli : -
    • Pseudomonas : +
  2. Catalase Test
    • Identify organism that produce Catalase.
    • Enzyme that breaks hydrogen peroxide H2O2 -> H2O & O2 (Bubble : +)
    • Staphylococcus aureus : +
    • Enterococcus : -
  3. Nitrate Reduction Test
    • used to determine the ability of an organism to reduce nitrate -> nitrite using enzyme nitrate reductase.
    • NO3 -NitrateReductase-> NO2
    • E.Coli : +
    • Bacillus : +
    • Pseudomonas : +
  4. Phenol Red Broth
    • used to differentiate gram negative enteric bacteria.
    • Phenol red is a pH indicator which turns
    • yellow < pH 6.8 and fuchsia > pH 7.4
    • E.Coli : +
    • Proteus : -
  5. Methyl Red (MR) and Veges-Proskauer (VP)
    • If the organism uses the mixed acid fermentation pathway and produces stable acidic end-products.
    • The MR and VP tests are particularly useful in the identification of the Enterobacteriaceae.
    • E.coli : + Red
    • Enterobacter : - No change(yellow)
  6. Starch Hydrolysis
    • To identify the organism to produce amylase that hydrolyze starch.
    • Bacillus : +
  7. Citrate Test
    • To tests the ability of organisms to utilize citrate as a carbon source (Green : -, Blue : +)
    • E.Coli : -
    • Enterobacter : +
  8. Sulfur Reduction
    • If an organism can reduce sulfur to hydrogen sulfide, the hydrogen sulfide will combine with the iron to form ferric sulfide, which is a black precipitate. If there is any blackening of the medium, it indicates the reduction of sulfur and is a positive result.
    • Black : +
  9. Indole
    • Ability of organism to produce Indole using enzyme tryptophanase.
    • The Kovac’s reagent is added to the SIM medium to test for indole
    • Pink Ring : + (E.coli)
    • Clear Ring : -
    • Enterobacter : -
  10. Motility Test
    • The preferred way to determine motility is to use the semi-solid medium
    • If the growth around the stab line has radiated outwards in all directions or if the entire tube has turned pink, your organism is motile.
  11. Gelatinase Test
    • To tests the ability of an organism to produce an enzyme called gelatinase, that hydrolyzes gelatin.
    • Liquid : +
    • Staphyloccus Aureus : +
  12. Urease Test
    • To test the ability of an organism to produce an enzyme, called urease that hydrolyzes urea to ammonia & carbon dioxide.
    • Pink : + (Proteus)
    • Yellow : - (E.coli)

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