Meeting 15

Card Set Information

Author:
mse263
ID:
116035
Filename:
Meeting 15
Updated:
2011-11-11 00:30:03
Tags:
MCBI Exam
Folders:

Description:
7.5 7.7
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user mse263 on FreezingBlue Flashcards. What would you like to do?


  1. transcription by RNA pol II requires several initiation factors. These factors:
    • -position polymerase at Tx start sites
    • -help melt DNA strands

    • -some: TFIIA, TFIIB, and TFIID
    • -TFIID is the largest and consists of TBP and 13 TAFs (TBP-associated factors)
  2. preinitiation complex
    complex of RNA pol II and its general Tx factors bound to a promoter and ready to initiate Tx
  3. TBP (TATA-box-binding protein)
    • -1st protein to bind to a TATA-box promoter
    • -interacts with DNA's minor groove to bend the helix
    • -C-terminal domain is pretty conserved (180 residues) while the N-terminus varies
  4. everything that happens after TBP has bound:
    • -once TBP has bound to promoter, TFIIB can bind
    • -after TFIIB is bound, a preformed complex of TFIIF and RNA pol II attaches
    • -then TFIIE binds, creating a place for TFIIH to bind
    • -TFIIH subunits do 2 things: 1) one unwinds DNA so RNA pol can begin Tx and 2) a second phosphorylates the pol II CTD
  5. Another Tx factor is required for initiation by Pol II in vivo: TFIIA
    TFIIA forms a complex with TBP and TATA-box DNA
  6. TAF subunits
    -the subunits of TFIID seem to play a role in initiating Tx from promoters that LACK a TATA box
  7. downstream promoter element (DPE)
    facilitates Tx of TATA-less genes that contain it by increasing TFIID binding (and subsequently TAF activity)
  8. could do 7.7 or just review if you have time

What would you like to do?

Home > Flashcards > Print Preview