BIOTEC E2 C22

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Author:
shockwave
ID:
117449
Filename:
BIOTEC E2 C22
Updated:
2011-11-17 01:12:57
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BIOTEC E2 C22
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BIOTEC E2 C22
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  1. WHAT CHROMOSOME AND GERE ARE YOU LOOKING AT ?
    • 22
    • TISSUE PLASMINOGEN ACTIVATOR (TPA)
  2. ALU ELEMENTS HAVE HOW MANY BP?
    300
  3. HOW DID IT GET ITS NAME?
    SINGLE RECONGINATION SITE FOR THE ENDONUCLEASE ALUI NEAR THE MIDDLE OF THE ALU ELEMENT
  4. OVER THE LAST ---YEARS, THE ELEMENT HAS AMPLIFIED VIA --- --- -- ----- TO COPYNUMBER OF ABOUT ------.
    65 MILLION

    RNA MEDIATED TRANSPORTATION PROCESS

    500,000
  5. THE ALU COMPRISES ABOUT ---- OF THE HUMAN GENOME
    5%
  6. THE ALU IS DERIVIED FROM WHAT GENE?
    • 7SL RNA GENE.
    • THIS GENE ENCODES THE RNA COMPONENT OF THE SIGNAL RECGONIZATION PARTICLE THAT FUNCTIONS IN PROTEIN SYNTHESIS.
  7. AN ESTIMATED ---- TO ---- ALU ELEMENTS ARE RESTRICTED MIOSTLY TO THE HUMAN GENOME
    500-2,000
  8. T OR F
    THE ALU SEQUENCE IS FOUND WITHIN A INTERON IT DOES NOT EFFECT THE TPA 25 EXPRESSION AND THUS PHYNOTYPIC NEUTRAL.
    TRUE
  9. NAME THE 2 SIZES OF THE OLIGONEUCLITE PRIMERS
    • 400 PRESENT
    • 100 NOT PRESENT
  10. NAME THE 3 POSSIBLE GENOTYPES
    • HOMOZYGOTES (POS) 400BP
    • HOMOZYGOUS (NEG) 100 BP
    • HETERZYGOTES 400 & 100 BP
  11. WHAT IS THE NAME OF THE RESIN USED AND WHY IS IT USED?
    • CHELEX.NEG CHARGE.
    • BINDS TO POS METAL IONS AND PREVENTS PCR REACTION. (Mg)
  12. INGREDIENTS OF THE MASTER MIX
    • PCR BUFFER 10X
    • dNTP's 10mM
    • UPSTREAM PRIMER ('5 G)
    • DOWNSTREAM PRIMER ('5 C)
    • Taq POLYMERASE
    • H20
    • YOUR DNA
  13. WHAT IS THE BAD AT THE BOTTOM OF THE GEL CALLED AND IS COMPOSED OF WHAT?
    • PRIMER-DIMER.
    • COMPOSED OF PRIME-PRIME AMPLIFICATION PRODUCT.
  14. A LAMDA / BamHI+HindIII IS RUN IN THE SAME GEL. WHY?
    493 & 125 BASE PAIRS. USED AS GUIDE.

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