Clinical Chemistry 2

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anubis_star
ID:
118807
Filename:
Clinical Chemistry 2
Updated:
2011-11-27 13:30:46
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Clin Clinical Chem Chemistry Unit Two Bel Rea
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Description:
Clin Clinical Chem Chemistry Unit Two Bel Rea COMPLETE
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    • Griffin beaker
    • Various sizes
    • Graduated
    • NOT accurate for measuring volume
    • Used for holding and mixing solutions
    • Erlenmeyer flask
    • Various sizes
    • Graduated
    • NOT accurate for measuring volume
    • Used for holding and mixing solutions
    • Volumetric flask
    • Measures ONE volume only
    • Increased accuracy
    • Increased glass volume
    • Graduated cylinder
    • Various sizes
    • Graduated
    • Increased accuracy
    • Choose smalles that'll hold
    • Cuvette
    • Fancy test tube
    • Increased glass quality
    • Measure in spectrophotometry by measuring amount of light transmitted thru a solution or absorbed by a solution and calculating concentration
    • Plastic Transfer Pipette
    • Disposable
    • NOT used for measuring
    • Pasteur Pipette
    • Transfer Pipette
    • Glass with a bulb, disposable
    • Remove serum or plasma from tube of blood
    • NOT for measuring
    • Serological pipette
    • Glass
    • Graduated
    • Various sizes
    • Use with a bulb or pump
    • Fairly accurate, precise for most uses when measuring volume
    • Volumetric pipette
    • Glass
    • Bulge in the midde
    • Measures ONE volume only
    • Most accurate, but most $$$$$
  1. TC = ________________
    TD = ________________
    • TC = To Contain
    • TD = To Deliver
    • Automatic Pipette
    • AKA Micro Pipette
    • Measures very small quantities
    • Measures ONE volume only
    • Primarily in ul
    • Disposable tip
    • Microscope
    • Oculars magnify 10X
    • Objective lens magnify 4X, 10X, 40X, 100X
    • Final magnification - Oculars x Objective lens
  2. Blood is centrifuged ____________ rpm/ ________ min
    • 6000 RPM
    • 5 mins
  3. Urine is centrifuged ________ rpm/ ________ min
    • 1500 RPM
    • 3-5 Mins
  4. Wet Chem Analyzer
    Spectophotomoeter which measures CSF, TP
  5. Dry Chem Analyzer
    • Vet Test and AEVH, IDEXX
    • $$$$$
    • Measures glucose, electrolytes, ALT, UN, creatinine
  6. Automated Hemo Machines
    • Mascot
    • CBC Autoreader
  7. Wright's Stain/Diff Quick
    Hemo, cytology
  8. NMB
    • New Methylene Blue
    • Hemo, retics, cytology, U/A
  9. Sedi-stain
    U/A
  10. Gram Stain
    Micro (bacteria)
  11. Lugol's Iodine
    Fecals
  12. Lactophenol Cotton Blue
    Micro (molds)
  13. Incubators
    • Micro
    • Culture/grow
  14. Water Baths
    Hemo (TP:F)
  15. Quality Control
    • Quality Assurance
    • Ensures the accuracy of results
  16. Log of quality control important because
    • AAHA requirements
    • Detect trend in values
  17. 2 general ways to preserve pt. specimens
    • Decrease Temp
    • Chemical
  18. Most blood chemistries are run on ______ to avoid _________ interference
    (the exception is __________)
    • Serum
    • Anticoagulant
    • Lithium Heparin
  19. Colorimetry
    Lab technique used to measure concentration of a solution by the intensity of it's color
  20. Beer's Law
    The concentration of a solution is directly proportional to the amount of light absorbed by that solution and is inversly proportional to the amount of light transmitted thru the solution
  21. 4 different cuvettes needed for the spectrophotometer
    • 1. Blank
    • 2. Standard
    • 3. Control
    • 4. Patient
  22. Blank cuvette
    Contains reagent only. Used to Calibrate the machine to 100% transmitence.
  23. Standard Cuvette
    Standard solution only that has known concentration
  24. Control Cuvette
    Reagent + control solution, has an acceptable range of values
  25. Patient Cuvette
    Reagent + Serum

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