Manual Hematological Procedures
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Proportion of RBC's in whole blood
Fe containing pigment in the RBC's that carry oxygen
abnormal increase in RBC's in circulation that occurs secondary to disorders
RBC indices (aka red cell absolute value)
measurement that describes the size and Hgb content of the RBC's. Used in the differential diagnosis of anemia.
mean corpuscular volume (MVC)
volume of corpuscles expressed in microliters or femtoliters
-formula (Hct. * 10) / (RBC count)
measure of Hgb content of red cospuscles
mean corpuscular Hgb concentration (MCHC)
measure of concentration of Hgb in average RBC
Hgb% x 10 / RBC count
hematocrit determination done on an extremely small amount of blood by use of high speed centrifuge
device which counts concentration of cells in body fluid (eg. RBC's and WBC's in blood)
method for diluting blood in preparation for counting blood cells
procedure in which living tissue is removed from the body and placed in a non-toxic dye solution
decreased oxygen delivery to the tissues
bluish-gray color of younger RBC's.
sign of very active bone marrow
variation of RBC size
variation in shape of RBC's
platelets attach themselves to neutrophils in EDTA tubes.
leads to low platelet counts
excess of RBC's in peripheral blood
group of RBC's arrange in "role of coin" patterns.
due to abnormal protein coating on RBC's
seen in multiple myeloma and waldenstroms macroglobulinemia
at what rate should the microhematocrit rotor be placed when determining Hct.
14,500 RPM for 5 min.
the Hct. duplicate results should be within ____% of eacother?
reference range of Hct. for an adult male?
42 - 52%
reference range of Hct. for an adult female?
37 - 47%
reference range of Hct. for an infant?
30 - 43%
reference range of Hct. for a newborn?
53 - 65%
Hgb is oxidised to methemoglobin by which reagent?
Drabkins (Potassium ferricyanide)
reference range of Hgb. for an adult male?
14 - 17 g/dL
reference range of Hgb. for an adult female?
12 - 16 g/dL
reference range of Hgb. for an infant?
9 - 14 g/dL
reference range of Hgb. for a newborn?
17 - 23 g/dL
the Hgb. in patient with sickle cell anemia tends to?
crystalize, thus falsely elevating the Hgb. value
to compensate for the false increase in turbidity of sickle cell anemia patients, the sample must first be diluted using what?
what method is used for a WBC count?
in the unopette method, what 3 diluents are used?
ammonia oxalate (lyses, dilutes, preserves WBC's)
sorensens buffer (maintains pH)
reference range for WBC for an adult?
4.8 - 10.8 E^3/mm^3
reference range for WBC for a newborn?
9.0 - 30.0 E^3/mm^3
during a WBC count, why would NRBC's be a problem?
hematocyte cannot tell a WBC from a NRBC
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