Activity 15: Transformation Experiment (Handout)
•Clinical significance of transformation behind bacteria
•Some bacteria has the natural ability to take up naked DNA molecules from the environment, in other words they are “competent.” Many others including E. coli, are not naturally competent, but they can be made competent via laboratory manipulation. The ability to manipulate E. coli to be transformed competent, and hence take up naked DNA, revolutionized the field of molecular biology. For example, human insulin genes have been introduced into E. coli, and strains of this bacterium now human insulin or humulin.