Phloem Lecture 1

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Author:
cornpops
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120189
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Phloem Lecture 1
Updated:
2011-12-01 22:36:58
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PMB 135 exam6
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plant physiology and biochemistry exam 6
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  1. What gets transported from leaf to root through the phloem?
    • sucrose
    • amino acids
    • some ions
  2. What are the structural components of phloem transport?
    • conducting cells:
    • - sieve elements
    • - sieve cells (gymnosperms)
    • - sieve tube cells (angiosperms)
    • companion cells
    • parenchyma
    • fibers
    • laticifers
  3. *sieve elements
    • living cells highly specialized for translocation
    • lose their nucleus and vacuole
    • retain modified mitochondria, plastids, and smooth endoplasmic reticulum
  4. sieve areas
    • parts of the cell wall with pores
    • lateral transport
  5. sieve plates
    • found at the end walls of sieve tube cells
    • have larger pores
  6. *companion cells
    • adjacent to sieve cells
    • connected through plasmodesmata
    • densely cytoplasmic and have numerous mitochondria
    • take over metabolic functions of sieve tube

    • 3 types:
    • companion cell
    • intermediary cell
    • transfer cell
  7. *transfer cells
    • has finger like wall in growths - increase the surface area
    • specialized for taking up solutes
  8. intermediary cells
    use symplastic transfer of solutes
  9. evidence for the sieve tube as the transporting cell
    • 1) application of radioactive label:
    • 14CO2 supplied to leaf
    • 16C- sucrose supplied to leaf
    • 2) localization of radioactive label in transport cells - autoradiography
  10. autoradiography
    • 1) rapidly freeze
    • 2) freeze-dried
    • 3) embedded in resin
    • 4) sliced into thin sections
    • 5) coated photographically
    • 6) expose
    • 7) silver grains appear (saw in sieve tube)
  11. P-protein
    • phloem protein
    • forms a gel
    • puncture repair substance
  12. wounding response
    • P-protein blocks sieve plate pores
    • callose forms (callase enzyme)

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