CellBioL Final

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CellBioL Final
2011-12-05 17:57:28
cell bioL

cell bioL
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  1. How are cells put into culture?
    Primary and Sub-culturing
  2. What is primary culture?
    1. Explant culturing: small pieces of tissue are attached to a culture dish and bathed in culture medium. 2. Enzymatic dissociation: speeds up process by adding digesting enzymes to dissolve the cement holding cells together. They are then placed into a medium.
  3. What are the different types of cell culture?
    Fibroblast-like, epithelial-like, and lymphoblast-like
  4. What are Epithelial-like cell cultures?
    • cells that are attached to a substrate and appear
    • flattened and polygonal in shape.
  5. what are Lymphoblast-like cell cultures?
    Cell that don't attach normally to a substrate but remain in suspension with a spherical shape
  6. what are Fibroblast-like cell cultures?
    • cells that are attached to a substrate and appear
    • elongated and bipolar, frequently forming swirls in heavy cultures.
  7. What are the optimum conditions for cell growth?
    Controlled temp, Good substrate for cell attachment, appropriate medium, incubator (maintains pH & osmolality)
  8. Describe how you would practice sterile technique.
    Gloves, Pre-sterilze(alcohol), Prevent wafting, Sterile pipets, Fume hood
  9. Describe the steps of enzyme cytochemistry
    Incubation with a special colorless enzyme substrate
  10. what is Fixation?
    Fixed by immersion in ethanol or acetone. Makes them permeable to stains. Causes cells to adhere to slides and fix them in place
  11. What is Incubation with a special colorless enzyme substrate
    • Enzyme catalyzes the conversion of the subrate to a
    • colored product
  12. what is Detection of the colored product by microscopy?
    Colored product deposited at the site of enzyme action.
  13. Describe how you visualized the location of specific enzymes. Are you directly visualizing the enzyme itself?
    • Enzyme action is colored and allowed for visualization
    • via microscope. Can not visualize the enzyme itself, just its location
  14. What type of receptors did you study in lab? How did you detect the location of these receptors in epithelial cheek cells?
    • Cell surface receptors and Lectin receptors
    • observed the purple staining indicated surface receptors. detected lectin receptors by clumping
  15. How did you determine what sugar residues the ligands are binding to on the receptor?
    coloration and clumping