Final bio quiz
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Semiconservative replication involves a template. What is the template?
one strand of the DNA molecule
What is the difference between the leading strand and the lagging strand in DNA replication?
The leading strand is synthesized continuously in the 5′→3′ direction, while the lagging strand is synthesized discontinuously in the 5′→3′ direction.
What is a major difference between eukaryotic DNA replication and prokaryotic DNA replication
Prokaryotic chromosomes have a single origin of replication, while eukaryotic chromosomes have multiple origins of replication
What is a telomere?
the ends of linear chromosomes
Researchers found E. coli that had mutation rates
100 times higher than normal. What is a possible explanation for these results?
The proofreading mechanism of DNA polymerase was not working properly
In the Meselson-Stahl experiment, cells were grown for one generation in growth medium containing a relatively heavy nitrogen isotope, then transferred to new medium containing a lighter nitrogen isotope. After another round of cell division, what is true of the DNA in the cells?
All DNA contains a hybrid of heavy and light nitrogen isotopes
Which strand would be synthesized from the following portion of a DNA molecule? 3'
5' TTGGCATTAAGC 3'
Which of the following represents the correct order of events as they occur in the
process of DNA replication?
Helicase opens helix, DNA polymerase III elongates primer and synthesizes leading and lagging strands, DNA ligase links fragments of DNA.
Which of the following enzymes is correctly matched to its function?
DNA polymerase III—adds nucleotides to the lagging strand
In humans, xeroderma pigmentosum is a disorder of the nucleotide excision repair mechanism. These individuals are unable to repair DNA damage caused by ultraviolet light. Which of the following are the most prominent types of mutations in individuals
suffering from xeroderma pigmentosum?
Genotype is to _____ as phenotype is to _____.
DNA base sequence; amino acid sequence
In the Hershey-Chase experiment, bacterial cultures infected with virus were agitated in a kitchen blender to _____.
separate viral protein coats from the bacterial cells in the culture.
How does the simple primary and secondary structure of DNA hold the information
needed to code for the many features of multicellular organisms?
The base sequence of DNA carries all the information needed to code for proteins.
What provides the energy for the polymerization reactions in DNA synthesis
the deoxyribonucleotide triphosphate substrates
At the beginning of Wednesday's lecture, Dr Gee told you a story about dropping and breaking his wife's _____________.
Bodnar et al. (1998) used telomerase to
extend the life span of normal human cells. Telomere shortening puts a limit on the number of times a cell can divide. How might adding telomerase affect cellular aging
Telomerase ensures that the ends of the chromosomes are accurately replicated and eliminates telomere shortening.
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