Methods

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Author:
p.reilly1227
ID:
123138
Filename:
Methods
Updated:
2011-12-12 16:41:38
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Methods 567
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methods
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  1. Genomic analysis techniques (6)
    • Southern blot - finds specific DNA sequence
    • Clone plasmid
    • Genome library
    • cDNA library
    • Screen library - find colonly with recombinant that has DNA seq of interest
    • Sequencing - Sanger , 454 PYRO
  2. Gene trx analysis of DNA (3)
    • Promoter Deletion: MAP promoter elements
    • Linker scanning mutation analysis: Map control elements
    • Site directed mutagenesis: assay prot fxn via point mutation
  3. gene trx analysis of trx level (4)
    • RNase Mapping Assay: measure trx levels
    • RT-PCR
    • qRT-PCR
    • Northern Blot
  4. TF analysis: TF - DNA interaction techniques (3)
    • Gel-Shift Assay: use to find concensus sequences
    • EMSA: Column chromatography to find possible TF
    • DNase 1 footprint assay: Known control element + putitive TF
  5. TF analysis: protein analysis (2)
    • Mutational analysis: TF domain discovery
    • Yeast two-hybrid technique: protein protein interaction + protein bind site with reporter gene plasmid for TF +/- ID
  6. Epigenetic analysis
    ChIP assay: Protein-DNA interaction in context of in vivo chromatin state

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