Clinical Chem-urinalysis

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Author:
maravi80
ID:
123433
Filename:
Clinical Chem-urinalysis
Updated:
2011-12-13 16:32:25
Tags:
Clinical Chemistry
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Description:
Clincical Chemistry
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  1. What is the Strip Ph principle?
    • Double indicator system
    • Methy red-4.4-6.6
    • Bromothymol blue-6-7.6
  2. What are interferences for strip Ph?
    Old urines give a false alkaline due to ammonia formation.

    Falsely acidic with protein runover
  3. What is the principle of strip protein?
    Protein error of indicators
  4. What are the false positives for Protein?
    • detergents
    • leaching
    • high alkalline urine because it overrides the buffer
  5. What type of globulins originate in the urinary tract?
    Tamm Horsfall protein
  6. What are causes of Proteinuria?
    • Prerenal- myoglobin, hemoglobin, bence jones protein.
    • Renal- glomerula disease
    • glomerular damage
    • preeclampsia
    • exercise, fever, extreme cold, orthostatic
    • Postrenal- inflammation malignancy, bladder injury
  7. What can detect early signs of kidney problems?
    albumin in urine
  8. What are three techniques to measure albumin in urine?
    • Random urine- normal <30-300 ug albumin/mg creatinine
    • 24 hour collection- 30-300mg
    • Timed specimen to determine albumin excretion- 20-200 mg/min
  9. What is orthostatic proteinuria?
    Protein positive during day when standing up and negative when asleep or laying down.
  10. Classic test for Bence Jones Protein?
    • Coagulates and precipitates at 40-60 C
    • Dissolves at 100 C
    • Filter and observe for turbidity at 40-60C
  11. What is significance of Bence jones proteins?
    multiple myeloma
  12. What is principle of glucose strip?
    uses glucose oxidase method with peroxidase
  13. What are false negatives for glucose?
    • ascorbic acid
    • aspirin
    • high bacteria count
    • low temp or high ketones
    • homogentisic acid
  14. What are fase positives for urine glucose?
    bleach
  15. What is the principle of clinitest for glucose?
    ability of glucose to reduce copper sulfate to cuprous oxide in presence of heat and alkali.
  16. What are interfenrences of clinitest?
    Other reducing sugars like lactose, fructose, galactose, maltose, pentose, ascorbic acid
  17. What are ketones?
    Products of fat metabolism
  18. What are the three types of ketones?
    • 2% acetone
    • 20% acetoacetic acid-detected with sodium nitro prusside
    • 78% beta hydorxybutyric acid-not detected
  19. False negatives with ascorbic acid?
    • Blood
    • bilirubin
    • Nitrite
  20. False negative for Nitrite?
    • Vegatable diet
    • ascorbic acid
    • not all bacteria react

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