CELL BIO 370 E1 C18

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shockwave
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135190
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CELL BIO 370 E1 C18
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2012-02-14 13:30:07
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CELL BIO 370 E1 C18
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CELL BIO 370 E1 C18 GSU 2012
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  1. T OR F resolution IS THE ABILITY TO SEE TWO NEIGHBORING POINTS DISTINCT FROM EACH OTHER
    TRUE
  2. resolution is limited by the _____ _____ _______.
    wavelength of light (1000X)
  3. A ____________ ___________ IS THE MEASUREMENT OF LIGHT GATHERING QUALITIES OF THE LENS
    NUMERICAL APERTURE
  4. t or f ?
    lens with greater resolving power have shorter focal lengths
    true
  5. what microscope required for the specimen and background have different refractive indices
    light microscope
  6. WHAT MICROSCOPE UTILIZES INTERFERENCE TO CONVERT THE DIFFERENCES IN THE REFRACTIVE INDEX OF SOME PARTS OF THE SPECIMEN TO DIFFERENCES IN LIGHT INTENSITY?
    PHASE CONTRAST
  7. DEFINE DIFFERENTIAL INTERFERENCE
    PART OF INTERFERENCE MICROSCOPE TYPES OPTICS THAT GIVE A THREE-DIMENSIONAL QUALITY. ALSO CALLED MARKSKI OPTICS.
  8. NAME OF FLUOROCHROME CHROME AND HOW IT WOULD BE USED.
    • RHODAMINE OR FLUORESCEIN.
    • COLVALENTLY LINK (CONJGATE) LABEL TO DNA OR Ab (IMMUNOFLYORENCES) TO OBSEVE PROCESS.
  9. WHAT IS THE SOURCE OF GFP
    AEQUOREA VICTORIA
  10. DEFINE FRET
    • FLUORESCENCE RESONANCE ENERGY TRANSFER.
    • THINK PROTEIN FOLDING MEASUREMENTS IN nm.
  11. A RAZOR DREAM IS USED TO EXAMINE PLANS AT DIFFERENT DEPTHS IN THE SPECIMENS.... WHAT MICROSCOPY?
    CONFOCAL....THINK CAT SCAN
  12. WHAT TYPE?
    SUBATOMIC STRUCTURES WITH BIREFRINGENCE ROTATE PLANE POLARIZED LIGHT.
    POLARIZATION
  13. SHORT WAVELENGTHS ARE EMITTED BY LASERS AND FLOURCHROMIC EXCITATION LONG WAVELENGTHS ARE EMITTED AND CAPTURED… WHAT TYPE?
    FLUORESCENCE CONFOAL SCANNING LIGHT MICROSCOPE.
  14. THE LIMIT OF RESOLUTION OF STANDARD TRANSMISSION ELECTRON MICROSCOPY IS WHAT?
    3 TO 5 A
  15. HOW DOES A TRANSMISSION ELECTRON MICROSCOPE WORK?
    ELECTRON BEAM THRU TUNGSTON FILAMENT AT HIGH VOLTAGE CAUSE ELECTRON BEAM. FOUCUS WITH MAGNETIC FIELD. ELECTRONS PASS THRU SPECIMENT, STRICKE FLOURESCENT SCREEN TO MAKE IMAGE.
  16. IN THE TEM PROCESS DIFFERENTIAL SCATTERING OF THE ELECTRONS IS PROPORTIONAL TO WHAT?
    THE SPECIMENT THICKNESS
  17. 100 NAMOMETER (nm)
    = _______ MICROMETERS (MU m)
    1
  18. THE SAMPLE IS SPRAYED WITH HEAVY METALS TO OBATIN CINTRAST....WHAT TYPE?
    • TEM
    • TRANSMISSION ELECTRON MICROSCOPE.
  19. TEM SPECIMENT PROCESS...WHAT ARE THE STEPS?
    • 1. FIX:GLUTARADELHYDE & OSMIUM
    • 2. DEHYDRATE
    • 3. EPOXY EMBEDDING
    • 3. THIN GLASS CUT SECTIONS COLLECTED ON Fe GRID
  20. IN A NEGATIVE STAING, HEAVY METALS DEFUSE INTO SPACES BETWEEN SPECIMENT MOLECULES....WHAT MICROSCOPY?
    TEM
  21. HOW DO YOU CREATE A SHADOW EFFECT IN A TEM SPECIMENT?
    METAL EVAPORATED AT LOW ANGLE WHILE ITS ROTATED.
  22. WHO USED WHAT TECHINQUE TO UNDERSTAND THE PHOSLIPID BYLAER?
    1970 DANIEL BRANTON USED FREEZE FRACTURE.
  23. IF ONE WANTS TO MAKE A FREEZE-FRACTURE REPLICA, WHAT DO YOU DO?
    COAT THE SAMPLE WITH GEAVY MEATLS AND THEN WITH CARBON.
  24. WHAT IS THE DIFFERENCE BETWEEN A FREEZE-FRACTURE REPLICA AND A ETCHING?
    • YOU THAW THE ETCHING FIRST THEN USE HEAVY METALS AND COAT WITH CARBON.
    • NOTE: IN DEEP ETCHING YOU THAW SAMPLE A FEW MORE TIMES, IT GIVES GREATER DEPT.
  25. WHAT METALS IS USED IN SEM?
    CARBON THEN GOLD
  26. YOU WANT TO EXAMINE THE INTERNAL STRUCTURES OF A CELL WHAT DO YOU USE?
    TEM OR SEM
    TEM
  27. IN WHAT MICROSCOPY PROCESS TO YOU PASS THE SAMPLE THRU DIFFERENT ALCOHOLS AND THEN DEHYDRATE TO THE CRITICAL POINT OF DRYING?
    SEM
  28. THE GOAL OF SPECIMENT PREPERATION IS WHAT?
    TO PRODUCE AN OBJECT WITH THE SAME SHAPE AND SURFACE STRUCTURE AS THE LIVING STATE, BUT IS DEVOID OF FLUIDS.
  29. WHY CAN'T YPU JUST AIR DRY A SEM SAMPLE?
    CAN'T DUE TO THE SURFACE TENSION AT AIR-WATER INTERFACES.
  30. IN TEM, THE ELECTRON BEAM IS FOCUSED HOW?
    • BY THE CONDENSER LENS TO SIMULTANEOUSLY ILLUMINATE THE ENTIRE VIEWING FIELD.
    • THE BEAM PASSED THRU THE SAMPLE.
  31. WHAT IS THE DIFFERENCE IN HOW THE IMAGE IS PRODUCED IN TEM VS SEM
    • SEM: ELECTON BEAM SCANS ACROSS THE SAMPLE AND REFLECTION(BACKSCATTER) CREATES IMAGE. 2ND BEAM SCANS IT...SEND TO CRT..MAKES PIC. (INDIRECT)
    • TEM: WIDER BEAM GOES THRU THE SAMPLE AND CREATES IMAGES.
  32. WHAT METHOD OPERATES BY SCANNING A DELICATE PROBE OVER THE SURFACE AND HAS A TINY BEAM (CANTILEVER) THAT READS AND CREATES AN IMAGE DOWN TO THE MOLECULAR LEVEL.
    ATOMIC FORCE MICROSCOPE (AFM)

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