GEN #10 DNA Sequencing and Mutagenesis

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Author:
HUSOP2014
ID:
136330
Filename:
GEN #10 DNA Sequencing and Mutagenesis
Updated:
2012-03-02 08:26:48
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HUSOP GEN EXAM2 sequencing
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#10 DNA Sequencing and Mutagenesis
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  1. the most widely used DNA sequencing method
    Sanger
  2. Two general methods used for sequencing
    • Maxam and Gilbert
    • Sanger
  3. Which sequencing method uses chemical degradation of the original DNA
    Maxam & Gilbert
  4. The _________ sequencing method is used to confirm the _______ sequencing method
    • Maxam and Gilbert
    • Sanger
  5. Which sequencing method is used for analysis of DNA modifications
    Maxam & Gilbert
  6. The Sanger Method uses a ____________ to terminate chain elongation at a specific nucleotide
    dideoxynucleotide
  7. This mutagenesis method uses a ________ that has the appropriate mismatch located _________
    • primer
    • within it
  8. Oligonucleotide-mediated mutagenesis requires what 3 reagents
    • M13 bacteriophage (single strand) containing sequence to be mutated
    • Mutagenic oligonucleotide
    • Universal primer
  9. Allows one to insert a small sequence of altered bases into a sequence
    Oligonucleotide-mediated mutagenesis
  10. compression of the sequence occurs as the replicated DNA gets _________
    larger
  11. Which sequencing method readily gives nucleotide base sequences but not modifications
    Sanger
  12. sequencing details read in what direction
    bottom--> up in a 5' to 3' direction
  13. This mutagenesis method is limited to the mutation of just a few nucleotides at a time
    PCR-mediated mutagenesis
  14. This mutagenesis method uses a universal primer
    Oligonucleotide-mediated mutagenesis
  15. PCR-mediated mutagenesis uses a ____________ that has the appropriate mismatch located within it
    primer
  16. most DNA sequencing uses the ________ method
    Sanger
  17. Sanger cannot sequence areas that induce _______
    distortion
  18. Oligonucleotide-mediated mutagenesis prefers _________ DNA over __________ DNA.
    • single stranded
    • double stranded
  19. ___________ changes DNA sequences at a chosen location
    Mutagenic oligonucleotide
  20. The flanking region has a sequence that is __________ to the ______________
    • complementary
    • oligonucleotide

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