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What is the 40x objective?
another name for the high dry objective
What is a wet mount?
a way to mount a living suspension of organisms
Name two reason why we use a wet mount?
- see Motility
- cellular arrangements
- undistorted organisms
Name a tip for success when using a microscope?
- Always start out using the 10x
- dont turn the light all the way on
- always clean the objectives before using
What is Potato Dextros Agar?
Media used for the general growth of molds and fungi
What is differential Media?
Media that contains substances that allow certain groups of bacteria to appear different from others
What is complex media
- used for general isolation of organisms
- Media in which the exact chemial composition is not known
What is staph
the prefix for cellular arrangements in which the organisms are in clusters
Name two words usded to describe the form of a colony?
- round, circular
- irregular, rhizoid
- curled, spindle
The four types of sterilization?
- gaseous sterilization
What is aseptic technique?
a tecnhique used to reduce the amount of contaminants introduced to steril environments and cultures
Streak platingi purpose?
a mehtod of culturing bacteria that allows pure individual colonies to be grown
Calculate the CFU/ ml in the original sample?
your plate of dilution factor 10-4 grew 50 colonies
your plate od dilution factor 10
What is dilution plating?
- a method used to estimate the numbers of bacteria in a sample
- ex= soil, water, or food
The Ziehl Neelsen stain?
technique stains acid fast bacteria pink
Name the media that has both selective and differential components?
- mannitol fermentation
- high salt selects Gram +
- lactose fermentation
- bile selects Gram -
What is Gram Staining?
The staining procedure that differentiates between cells based on the composition of their cell walls
In cram staining this type of cell turn out to be a purplish color when the procedure is complete?
Gram positive cells
What is a capsule stain?
a negative satin
The shaeffer-fulton stain?
the stain that distingquishes between endospores and vegatative cells