BIO CELL 371 E1 C2

Card Set Information

Author:
shockwave
ID:
138145
Filename:
BIO CELL 371 E1 C2
Updated:
2012-02-28 10:20:42
Tags:
BIO CELL 371 E1 C2
Folders:

Description:
BIO CELL 371 E1 C2 GSU 2012
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user shockwave on FreezingBlue Flashcards. What would you like to do?


  1. MUCH OF WHAT IS KNOWN ABOUT THE COMPOSITION OF THE PLASMA MEMBRANE COMES FROM WHERE?
    STUDIES OF THE HUMAN RBC
  2. ERYTHOCYTE AMOUNT & SIZE
    • 4.5 - 5.5 MILLION/mm3
    • 7.5 um DIAMETER
    • 1.9 um THICKNESS
  3. MOST ABUNDANT LEUKOCYTE IN ADULTS
    • NEUTROPHILS
    • 55-75%
  4. NEUTROPHGILS SIZE
    9-12µm
  5. WHAT ARE THE LOBES IN A NEUTROPHIL CONNECTED BY?
    THIN STRAN OF CHROMATIN
  6. A YOUNG NEUTROPHIL WHICH DO NOT HAVE THERE NUCLEI DIVIDED INTO LOBES IS CALLED WHAT?
    BAND NUETROPHILS
  7. ANOTHER NAME FOR POLYMORPHONUCLEAR LEUKOCYTES.
    • GRANULATED LEUKOCYTES.
    • NEUTROPHIL, EOSINOHILS AND BASOPHILS.
  8. WHAT IS THE FIRST LEUKOCYTE AND AT WOUND SITE?
    NEUTROPHILS.
  9. WHO TARGETS BACTERIA AND FUNGI?
    NEUTROPHILS
  10. EOSINOPHILS....APPROX % IN ADULTS?
    1.5 %
  11. EOSINOPHILS SIZE
    10-14µm
  12. HOW ARE ENOSINPHILS NAMED?
    NAMED FOR THEIR TENDANCY OF THEIR CYTOPLASMIC GRANULES TO STAIN WITH ACIDIC DYES.
  13. THE GRANULES OF AN EOSINOPHILES ARE WHAT?
    LYSOSOMES
  14. WHAT WOULD GO AFTER PARASITES, ALLERGIC REACTIONS AND PROTEINS THAT ARE COMPLEX WITH ANTIBODIES?
    EOSINPHILS
  15. WHAT WBC GRANULES STAINS WITH BASIC DYES?
    BASOPHILS
  16. WHAT IS THE LEAST FREQUENT WBC TYPE?
    • BASOPHILES.
    • LESS 1 %
  17. WHAT BESIDES EOSONOHPHILS ATTACK ALLERGIC REACTIONS?
    BASOPHILES
  18. HOW BIG IS A BASOPHILE?
    12-15µm
  19. WHAT LLEUKOCYTE HAS HISAMINE AND HERPARIN IN ITS GRANDULES?
    BASOPHILE
  20. WHAT IS THE 2ND MOST ABUNDANT TYPE OF WBC? ALSO GIVE % IN AN ADULT.
    • LYMPHOCYTES
    • 20-40%
  21. SIZE RANGE FOR A LYMPHOCYTE?
    USUALLY 7-8µm....BUT CAN GOTO 15µm.
  22. WHERE DO T AND B CELLS COME FROM? AND DEFINE EACH TYPES FUNCTION.
    • BOTH FROM LYMPHOCYTE
    • B...VARIOUS PATHOGENS
    • T...
    • CD4 BREAK DOWN BACTERIA
    • CD8 VIRUS INFECTED AND TUMORS
  23. WHER DO NATURAL KILLER CELLS COME FROM?
    • LYMPHOCYTES.
    • ATTACK VIRUS INFECTED AND TUMOR CELLS.
  24. MONOCYTES SIZE AND % IN ADULTS
    • 12-15µm
    • 2-8%
  25. IDNETIFICATION:
    MONOCYTE
  26. IDENTIFICATION:
    BASOPHIL
  27. IDENTIFICATION:
    BASOPHIL
  28. IDENTIFICATION:
    EOSINOPHILE
  29. IDENTIFICATION:
    EOSINPHILE
  30. IDENTIFICATION:
    LYMPHOCYTE
  31. IDENTIFICATION:
    NEUTROPHILE
  32. IDENTIFICATION:
    T-LYMPHOCYTE
  33. WHO AM I?
    3-8% IN ADULTS
    12-15µm
    FIGHT CORNIC INFECTION
    FUNCTION MAINLY IN BODY TISSUES
    MONOCYTES
  34. IN VITAL STAINING, JANUS GREEN STAINS WHAT?
    MITOCXHINDRIA
  35. IN VITAL STAINING, NEUTRAL RED STAINS WHAT?
    • CYTOPLASMIC GRANULES
    • VESCILES
  36. WHEN JANUS GREEN BECOMES OXIDIZED, WHAT COLOUR DOES IT BECOME? AND WHY?
    • BLUE-GREEN
    • CYTOCHROME OXIDASE IN THE MITOCHONDRIA
  37. IN VITAL STAINING, IF THE JANUS GREEN STAIN WAS TO GO TO THE CYTOPLASM, WHAT WOULD HAPPEN AND WHY?
    IT WOULD GO COLORLESS, BECUAUSE THE CYTOPLASM IS A REDUCING ENVIORMENT.
  38. WHEN VITAL STAINING WITH JANUS GREEN, WHAT 2 WBC DO YOU SEE THE MITOCHONDRIA? WHAT COLOUR AND SIZE ARE THEY?
    • LYMPHOCYTES & MONOCYTES
    • (AGRANULAR LEUKOCYTES)

    • BLUE/GREEN RODS....1µm
    • (SOMETIMES AS SMALL SPHERES)
  39. IF YOU WANT TO OBSERVE A LIVING CELL WHAT STAIN DO YOU USE?
    • VITAL STAINING
    • (JANUS & NEUTRAL RED)
  40. NEUTRAL RED STINS WHAT?
    LYSOSOMES AND OTHER GRANULES IN SHADES OF YELLOW, ORANGE AND RED.
  41. NEUTRAL RED STAIN HAS THE GREATEST EFFECT ON WHAT?
    • EOSINOPHILES.
    • GRANULES IN CYTOPLASM STAIN ORANGE-YELLOW.
  42. WHAT IS PURPOSE OF FIXING A CELL? IN OTHER WORDS WHAT ARE YOU FIGHTING AGAINST?
    YOU WANT TO RENDERED THE CELL COMPONENTS INSOLUIBLE OR ELSE THE LYSOSOMES WOULD RELEASE HYDROLYTIC ENZYMES. ALSO PREVENTS SWELLING OR SHRINKAGE.
  43. WHAT CHEMICAL IS THE MAJOR COMPONENT OF THE FIXATIVE FOR STAINED FIXED BLOOD SMEARS?
    ABSOLUTE METHANOL
  44. WHAT STAIN IS USED IN THE FIXED SMEAR PROCEDURE? AND WHAT IS IT MADE OF?
    • WRIGHT-GIEMSA.
    • METHYL BLUE &METHYL AZURE
    • AND THE EOSINATES OF BOTH. THEN DILUTED IN METHANOL & GLYCEROL. THEN DILUTED IN AQUEUSBUFFER SOLUTION. pH OF 6-7.
  45. THE WRIGHT-GIEMSA STAINS WORK ON WHAT STRUCTURES AND AT WHAT pH?
    • pH 6-7
    • AZURES ACT AS BASES &
    • STAIN BASE STRUCTURES BLUE
    • EOSINS ACT AS ACIDS &
    • STAIN THE ACID-LOVING STRUCTURES RED.
    • (ACTUALLY THE VARIOUS PARTS STAIN PINK,PURPLE, RED & BLUE)
  46. WHAT IS AN ADVERAGE LEUKOCYTE COUNT?
    7,500/mm3

    • RANGE:
    • 5,000-10,000/mm3
  47. "ILIA" AND "OSIS" MEANS WHAT?
    AN INCREASE IN NUMBER (OF WBC)
  48. "ENIA" MEANS WHAT?
    DECREASE IN NUMBER (OF WBC)
  49. TO MANY WBC IS KNOWN AS WHAT?
    • LEUKOCYTOSIS
    • ABOVE 10,000/mm3
  50. AN INCREASED AMOUNT OF 50,000/mm3 OF WBC IS KNOWN AS WHAT?
    LEUKEMIA
  51. A DECREASE IN THE NUMBER OF CIRCULATING LEUKOCYTES BELOW THE LOWER LIMIT (5,000/mm3) IS KNOWN AS WHAT?
    LEUKOPENIA
  52. LEUKOPENIA, WHAT IS IT AND WHAT CAN CAUSE IT.
    • DECREASE IN THE LEUKOCYTES COUNT BELOW 5,000/mm3.
    • CAUSES: DEPRESSION OF BONE MARROW DUE TO RADIATION, POISONING OR ALCOHOLISM.
  53. is caused by protozoan infections or asplastic anemia
    (bone marrow does not produce sufficient new cells to replenish blood cells)
    neutropenia
  54. caused by strenuous exercise, rheumatic fever, severe burns
    neutrophilia
  55. caused by mumps, German measles, whooping cough
    lymphocytosis
  56. caused by scarlet fever parasitic infection or allergic reactions
    eosinophilia
  57. caused by chronic disease such as tuberculosis and leukemia
    monocytosis
  58. caused by the administration of glucocorticoid drugs
    lymphocytopenia
  59. a decrease in the number of circulating red blood cells below the normal range constitutes what?
    erythrocytopenia
  60. may occur with dehydration, shock, hypoxia or bone marrow cancers
    polycythemia
  61. HOW MANY TIMES DID YOU DILUTE RBC USING A HEMOCYTOMETER? WHAT DID YOU DILUTE IT WITH?
    • 200
    • HAYMANS OR GOWER'S SOLUTION
  62. WHAT IS THE NUMBER YOU USED TO CALCULATE THE RBC PER MILLMETER OF BLOOD FROM THE CENTER SQUARE?
    • 200 DILUTION
    • 10 DEPT OF CHAMBER
    • 25 AREA (1/25= 25)
    • 200 X 10 X 25 = 50,000
    • ...THEN TAKE YOUR COUNT FROM THE 5 SQUARES GET ADVERAGE AND MULTIPLY IT.
    • EX: 120 ADV /SQ * 50,000= 6M RBC PER mm3
  63. USING A HEMOCYTOMETER FOR WBC COUNTS WHAT WAS THE DILUTION FACTOR AND WHAT SOULTION WAS USED?
    • 20
    • TURK'S
  64. WHAT DOES TURK'S SOLUTION CONSIST OF?
    • 1 mL OF GLACIAL ACETIC ACID
    • 1 mL OF AQUEOUS 1% GENTIAN VOILET SOLUTION
    • 100 mL DISTILLED H20
  65. WHAT DOES TURK'S SOLUTION DO TO THE RBC AND THE WBC ?
    • RBC: THIS ACID SOLUTION LYSIS THE MEMBRANE AND CONVERTS HEMOGLOBIN TO HEMATIN.
    • WBC: GENTIAN VOILET STAINS IT, EASY TO SEE.
  66. FOR WBC COUNTS PER MILLIMETER OF BLOOD WHAT IS THE MULTIPLICATION FACTORS?
    • 20 DILUTION
    • 10 COUNTING CHAMBER DEPT (.1mm = 10)
    • 1 ADVERAGE # OF CELLS 1mm2 AREA.
    • 20 X 10 X 1 = 200
    • THEN TAKE ADV PER SQ AND MULTI.
    • EX: 120 ADV/SQ * 200 = 24,000 WBC PER mm2
  67. WHAT IS THE VOLUME OF THE WBC AND RBC ON THE HEMOCYTOMETER?
    • WBC = 0.1 cu mm
    • RBC = .004 cu mm
  68. WHEN A NEUTROPHIL PHAGOCYTOSIS A ANTIGEN, IT PRESENTS PARTS OF THE INVADER ON ITS COAT, WHY?
    SO THAT A B OR T CELL CAN COME ALONG, READ IT, AND MAKE Ab AGAINST IT.
  69. WHAT POWER DID YOU READ THE RBC COUNT AT?
    HIGH
  70. WHAT POWER DID YOU READ THE WBC AT?
    LOW
  71. NAME THE 2 TYPES OF GRANULES INA NEUTROPHILE
    • AZUROPHIL..PRIMARY..BPI
    • SPECIFIC..SECONDARY...LACTOFERRIN

What would you like to do?

Home > Flashcards > Print Preview