BIO CELL 371 E1 C3

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shockwave
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138280
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BIO CELL 371 E1 C3
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2012-02-28 01:36:59
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BIO CELL 371 E1 C3
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BIO CELL E1 C3 GSU 2012
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  1. WHEN THE CARBOXYL GROUP OF ONE AMINO ACID IS COVALENTLY LINKED TO THE ALPHA AMINO NITROGEN OF A SECOND AMINO ACID RESULTING COMPOUND IS CALLED WHAT?
    • DIPEPTIDE.
    • EVEN THOUGH IT CONTAINS ONLY ONE PEPTIDE BOND.
  2. THE LINKING OF THREE AMINO ACIDS YIELD A _________ WITH _________ PEPTIDE BONDS.
    • TRIPEPTIDE
    • TO
  3. THE DIRECT TEST IS COMMONLY USED TO DETECT THE PRESENCE OF PROTEINS AND PEPTIDES BY TREATING A SAMPLE WITH WHAT?
    • ALKALINE SOLUTION OF DILUTED COPPER SULFATE TO YOU A PINK VIOLET COLOR.
    • AT LEAST TWO PEPTIDE BONDS ARE REQUIRED FOR A POSITIVE TEST.
  4. HOW DOES THE BURET TEST WORK?
    Cu BIND TO PEPTIDE BONDS---> COLOUR CHANGE.
  5. WHAT TEST TO TEST FOR PROTEINS?
    BURETS
  6. WHAT TEST DO YOU RUN FOR BOTH ALPHA AMINO ACIDS AND PROTEINS?
    NINHYDRIN REACTION.
  7. A POSITIVE BURET'S TEST IS WHAT COLOUR?
    VIOLET
  8. A POSITIVE NINHYDRIN REACTION IS WHAT COLOR?
    INTENSE BLUE TO PURPLE.
  9. WHAT GET OXIDIZED IN THE NINHYDRIN REACTION?
    • THE AA.
    • NH3 GETS LIBERATED ON THE AMINE END
  10. WHAT WOULD RESULT IN A YELLOW NINHYDRIN REACTION
    PROLINE AND HYDROXYPROLINE ARE PRESENT. THEY HAVE NO ALPHA AMINO GROUP.
  11. XANTHOPROTEIC TEST FOR WHAT ?
    TYROSINE & TRYOPTOPHANE
  12. MILLONS TEST FOR WHAT ?
    TYROSINE
  13. SAKAGUCHI TEST FOR WHAT?
    ARGININE
  14. FOLINS TEST FOR WHAT ?
    CYSTINE
  15. BENEDICT'S REAGENT TEST FOR WHAT AND HOW?
    • TEST FOR CARBOHYDRATES (MONOSACCHARIDES AND SOME DISACCHRIDES).
    • THE FREE OR POTENTIALLY FREE ALDEHYDE OR KETONE ON GROUPS ARE READILY OXIDIZED BY MILD ALKYLENE SOLUTIONS OF CUPERIC IONS (COPPER SULFATE, SODIUM CARBONATE)
  16. the sugars that are involed in the bendict's reagent are known as ____ ______ because why?
    REDUCING SUGARS, THEY REDUCE THE CCuPERIC IONS FROM CUPRIC IONS TO FORM CUPROUS OXIDE (Cu2O). YELLOW TO BRICK RED PARCIPIATE.
  17. THE ALKALINITY OF THE BENDICTS SOLUTION IS WHAT AND WHY
    • 10.5
    • DUE TO THE HYDROLYSIS OF SODIUM CARBONATE
  18. WHAT DO YOU USE TO TEST FOR STARCH?
    • IODINE
    • AMYLOSE AND AMYLOPECTIN
  19. TRUE OR FALSE?
    LIPIDS ARE SOLUBLE IN NON-POLAR SOLVENT AND NOT IN AQUEOUS SOLUTIONS?
    TRUE
  20. ONE QUALITATIVE TEST FOR LIPIDS USES A FAT-SOLUBLE DYE, WHAT IS IT?
    SUDAN IV
  21. TRUE OR FALSE?
    THE NUCLEIC ACIDS ARE SO NAMED BECAUSE THEY WERE FOUND IN THE NUCLEI OF FISHBURNE BY MIESCHER ABOUT 50 YEARS AGO.
    TRUE
  22. BIAL'S ORCINOL REAGENT TEST FOR WHAT?
    • THE SUGAR RIBOSE IN RNA RESULTS IN GREEN, BLUE-GREEN COLOUR THAT YOU RUN IN A SPECTROMETER.
    • VERY ACIDIC.
  23. WHAT REAGENT DO YOU USE TO TEST FOR DNA?
    • DISCHE PHENYLAMINE (DIPHENYLAMINE).
    • POSITIVE IS BLUE.
    • IT REACTS WITH THE SUGAR.
  24. ORCINOL AND PHENYLAMINE ARE STONG ACIDS THAT HYDROLYZE WHAT?
    • THE PURINE NUCLOTIDES INTO SUGARS, BASES AND PHOSPHORIC ACIDS. IT FREES SUGARS FROM THE PURINE BASES ONLY! THEN REACT WITH THE COLOUR REAGENTS.(RNA = GREEN)(DNA=BLUE).
    • SUGARS DONT RELEASE FROM PYRMIDE BASE BECAUSE OF THE GLYCOSIDIC BOND,RESISTANT TO HYDROLYSIS.
  25. IN THE RNA ORICAL TEST, YOU HEAT THE RNA IN HCL. WHAT HAPPENS?
    DEHYDRATION OF RIBOSE TO FURFURAL. THE FURFURAL CONDENSES WITH ORCINOL IN THE PRESENCE OF FERRIC IONS TO FORM A VARIETY OF PRODUCTS WITH THE BLUE-GREEN COLOUR.
  26. IN THE DIPHENYLAMINE DNA TEST, YOU HEAT DNA IN HCL. WHAT HAPPENS?
    THE 2-DEOXYRIBOSE DEHYDRATES INTO HYDROXYLEVULINYL ALDEHYDE. IT CONDENSES WITH DIPHENYLAMINE TO FORM PRODUCTS THAT PRODUCE BLUE COLOURS.
  27. WHAT TEST FOR PROTEINS AND PEPTIDES?
    BIURET
  28. WHAT TEST FOR AA?
    NINHYDRIN REACTION
  29. WHAT TEST FOR CARBOHYDRATES?
    BENEDICT REAGENT
  30. WHAT TEST FOR STARCH?
    IODINE
  31. WHAT TEST FOR LIPIDS?
    • SUDAN IV
    • MORE LIKE A SOLUBILITY TEST THAN A PRESENCE TEST.
  32. WHAT TEST FOR RNA?
    ORCINOL
  33. WHAT TEST FOR DNA?
    DIPHENYLAMINE
  34. A POSITIVE BIURET TEST IS WHAT COLOUR?
    AN ALKALINE (BASIC) SOLUTION OF DILUTE COPPER SULFATE TO YIELD A YELLOW TO PINK/VIOLET COLOR.
  35. BIURWTS TEST. WHAT SPECIFIC CHM GROUPS RESPONSIBLE FOR TEST?
    DIPEPTIDE BONDS
  36. NINHYDRIN. WHAT SPECIFIC CHM GROUPS RESPONSIBLE FOR TEST?
    NH3 + CO2 + HYDRINDANTIN
  37. IODINE TEST.WHAT SPECIFIC CHM GROUPS RESPONSIBLE FOR TEST?
    glycosidic linkages
  38. BENEDICTS TEST.WHAT SPECIFIC CHM GROUPS RESPONSIBLE FOR TEST?
    REDUCING SUGARS. ALDOES AND KEYTOSE.
  39. ORCINOL TEST.WHAT SPECIFIC CHM GROUPS RESPONSIBLE FOR TEST?
    RIBOSE SUGARS . DEHYDROLIZE PURINES.
  40. DIPHENYLAMINE TEST.WHAT SPECIFIC CHM GROUPS RESPONSIBLE FOR TEST?
    DEOXYRIBOSE TURNS INTO HYDROXYLEVULINYL ALDEHYDE INSTEAD OF FURFURAL. IT REACTS WITH THE DIPHENYLAMINE AND PRODUCES BLUE COLOR.
  41. DEFINE SPONIFICATION
    THE ALKALINE HYDROLYSIS OF THE FATTY ACID ESTERS.
  42. WHAT HAPPENS IN SPONIFICATION?
    • TRIGLYCERIDES AND A BASE (ANYTHING -OH) REACT TO FORM A GLYCEROL AND A FATTY ACID SALT(SOAP).
    • ESTER LINKAGE UNDERGO HYDROLYSIS.
  43. SPONIFICATION NUMBER.
    BASICALLY WHAT IS WHERE AND WHY?
    • FAT (BUTTER AND LARD)..190-235..LONG TAILS
    • OILS (OLIVE, CORN SOYBEAN) 189-200..TAIL BENDS

    THESE NUMBERS ARE THE mg OF KOH IT WOULD TAKE TO SPONIFIACTE THE FAT.
  44. A FATTY ACID THAT IS SOLID AT RT.
    WHAT TYPE OF SPONIFICATION NUMBER WOULD IT HAVE? HIGH OR LOW?
    • HIGH.
    • FATS...SOLID...HIGH SPONIFICATION NUMNER
    • OILS...LIQUID....LOW SPONIFICATION NUMBER.
  45. WHAT DOES ALNINE LOOK LIKE?

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