# BIO CELL 371 E1 C5

 The flashcards below were created by user shockwave on FreezingBlue Flashcards. WHAT IS THE VCISABLE RANGE ? 380- 760 nm EXAMPLE OF SHORT WAVELENGTHS UV EXAMPLE OF LONG WAVELENGTHS INFARED (IR) QUALITATIVE VS QUANTITATIVE QUALITATIVE: PRESENT OR NOTQUANTITATIVE: HOW MUCH DEFINE ABSORPTION MAXIMA THE WAVELENGTHS AT WHICH THE ABSORPTION SPECTRIUM EXHIBITS A PEAK. WHAT'S THE SPEC 20 RANGE? 340-1000nmNEAR UV - VISIABLE-NEAR IR HOW DOES THE SPEC 20 WORK? TUNGSTON LAMP-->DEFRACTION GRATING (ADJUST VIA CAM)-->20nm BEAM HITS SAMPLE---->PHOTOTUBE READS BEAM.ABS AND TRANS READOUT. DEFINE TRANSMISSION THE FRACTION OF INCIDENT OR INCOMING LIGHT TRANSMITTED BY A SAMPLE.T = I/Io X 100 I=STRIKE/Io=AMOUNT AFTER STRIKE.( IN %) IF YOU HAD T HOW DO YOU GET A? A = LOG 1/T OR LOG Io/I (NOTE THAT THE LOG FORMULA IS INVERSE OF T FORMULA) AT 100% TRANSMITTANCE, WHAT WOULD A BE? 0A= LOG 1=0 HIGH T = LOW A WHAT ARE THE SCALES FOR T% AND A? T% = 100 TO 0 A = 0 TO INFINITY WHY PLOT A AND NOT T% USUALLY PLOT A AGAINST CONCENTRATION, IT GIVE A LINEAR LINE. T% GIVES A CURVE. WHEN YOU START, YOU HAVE A QUARTZ CUVETTE AND YOUR SOLVANT INSIDE OF IT, EXECPT FOR WHAT YOUR MEASURING. WHAT IS THE SETTING OF T% AND A ? T% = 100AND THUS A = 0 DEFINE BEER-LAMBERT LAW. ABSORPTION IS DIRECTLY PROPORTIONAL TO SOLUTE CONCENTRATION (C) AND THE LENGTH OF THE LIGHT PATH (L). WHAT IS THE BEER-LAMBERT FORMULA? E = ABSORPTION COEFFICENTE & C (CONCENTRATION) = GIVEN IN UNITS OF WEIGHT.L = 1cm (USUALLY) EGG WHITES CONTAIN BOTH _______ & _______. BOTH ARE SOLUBILE IN ________. BUT ONLY _________ ARE SOLUBLE IN _______. ALBUMINS & GLOBULINSSALINE (.85% NaCl)ALBUMINS ARE SOLUBILE IN WATER. Authorshockwave ID138319 Card SetBIO CELL 371 E1 C5 DescriptionBIO CELL 371 E1 C5 GSU 2012 Updated2012-03-20T15:50:24Z Show Answers