BIO CELL 371 E1 C5

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Author:
shockwave
ID:
138319
Filename:
BIO CELL 371 E1 C5
Updated:
2012-03-20 11:50:24
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BIO CELL LAB E1 C5
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BIO CELL 371 E1 C5 GSU 2012
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  1. WHAT IS THE VCISABLE RANGE ?
    380- 760 nm
  2. EXAMPLE OF SHORT WAVELENGTHS
    UV
  3. EXAMPLE OF LONG WAVELENGTHS
    INFARED (IR)
  4. QUALITATIVE VS QUANTITATIVE
    • QUALITATIVE: PRESENT OR NOT
    • QUANTITATIVE: HOW MUCH
  5. DEFINE ABSORPTION MAXIMA
    THE WAVELENGTHS AT WHICH THE ABSORPTION SPECTRIUM EXHIBITS A PEAK.
  6. WHAT'S THE SPEC 20 RANGE?
    • 340-1000nm
    • NEAR UV - VISIABLE-NEAR IR
  7. HOW DOES THE SPEC 20 WORK?
    • TUNGSTON LAMP-->
    • DEFRACTION GRATING (ADJUST VIA CAM)-->
    • 20nm BEAM HITS SAMPLE---->
    • PHOTOTUBE READS BEAM.
    • ABS AND TRANS READOUT.
  8. DEFINE TRANSMISSION
    • THE FRACTION OF INCIDENT OR INCOMING LIGHT TRANSMITTED BY A SAMPLE.
    • T = I/Io X 100
    • I=STRIKE/Io=AMOUNT AFTER STRIKE.( IN %)
  9. IF YOU HAD T HOW DO YOU GET A?
    • A = LOG 1/T
    • OR LOG Io/I
    • (NOTE THAT THE LOG FORMULA IS INVERSE OF T FORMULA)
  10. AT 100% TRANSMITTANCE, WHAT WOULD A BE?
    • 0
    • A= LOG 1=0

    HIGH T = LOW A
  11. WHAT ARE THE SCALES FOR T% AND A?
    • T% = 100 TO 0
    • A = 0 TO INFINITY
  12. WHY PLOT A AND NOT T%
    USUALLY PLOT A AGAINST CONCENTRATION, IT GIVE A LINEAR LINE. T% GIVES A CURVE.
  13. WHEN YOU START, YOU HAVE A QUARTZ CUVETTE AND YOUR SOLVANT INSIDE OF IT, EXECPT FOR WHAT YOUR MEASURING. WHAT IS THE SETTING OF T% AND A ?
    • T% = 100
    • AND THUS
    • A = 0
  14. DEFINE BEER-LAMBERT LAW.
    ABSORPTION IS DIRECTLY PROPORTIONAL TO SOLUTE CONCENTRATION (C) AND THE LENGTH OF THE LIGHT PATH (L).
  15. WHAT IS THE BEER-LAMBERT FORMULA?
    • E = ABSORPTION COEFFICENT
    • E & C (CONCENTRATION) = GIVEN IN UNITS OF WEIGHT.
    • L = 1cm (USUALLY)
  16. EGG WHITES CONTAIN BOTH _______ & _______.
    BOTH ARE SOLUBILE IN ________.
    BUT ONLY _________ ARE SOLUBLE IN _______.
    • ALBUMINS & GLOBULINS
    • SALINE (.85% NaCl)
    • ALBUMINS ARE SOLUBILE IN WATER.

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