Clinical Chemistry

photons of energy that travels in waves

beam of electromagnetic radiant energy
linear distance traveled by one complete wave cycle

speed of a wave cycle

# of wave cycles occurring/second
Formula: v = c/ λ

atoms, ions or molecules have ability to absorb photons
of light

λ at which molecule will absorb maximum amt. of energy

as “excited” ions, atoms or molecules return to ground state

What are the three forms of relesed energy?

ratio of transmitted radiant energy (Is)/radiant incident light on sample (I)

contains everything but solute being measured
sets a new intensity of light Io

What is the amt. light absorbed dependent on?

The concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the logarithm of the transmitted light.

The ratio of radiant energy transmitted divided by the radiant energy incident on the sample.

What are the lamps most commonly used for the spectrophotometer? (visible and near-infrared)

What are the lamps most commonly used for ultraviolet work on the spectrophotometer?

Name the part by its function:
Isolation of individual wavelengths of light.

What are some examples of monochromators?

Convert the transmittance radiant energy into an equivalent amount of electrical energy.

What are some examples of photodetecters?

Contain a negatively charged cathode and a positively charged anode enclosed in a glass case. The cathode is composed of a material that acts as a resistor in the dark but emitts electrons when exposed to light.