GEN #15 Therapeutic Recombinant Proteins

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HUSOP2014
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139078
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GEN #15 Therapeutic Recombinant Proteins
Updated:
2012-03-02 08:16:30
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HUSOP GEN EXAM2
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GEN #15 Therapeutic Recombinant Proteins
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  1. Fusion proteins protect expressed protein by degradation by host cell ____________
    proteases
  2. _______ _________ enhance the solubility of the expressed foreign protein
    fusion proteins
  3. Fusion proteins increase the ease of purification using _________ _____________
    affinity chromatograph
  4. Must remove the additional ___________ which would be antigenic in vivo prior to final formulation
    peptide
  5. Many times the physiological ________, __________, is used to remove the peptide
    • protease
    • thrombin
  6. __________ is a peptide with affinity for streptavidin
    Strep-tag
  7. MBP stands for
    maltose binding protein
  8. GST stands for
    glutathione S-transferase
  9. _______ is a peptide recognized by enterokinase
    Flag
  10. Clone cDNA of interest into a _________ expression ________
    • His-tag
    • vector
  11. Run extract over a ____ ________column
    Ni++ affinity
  12. Digest purified protein w/purified ________
    thrombin
  13. Run over ____ _______ _______ to separate His polypeptide from _______ _______
    • size exclusion column
    • expressed protein
  14. Interferon-α/β is an early ________ _______ developed for the treatment of ______ _____ _________
    and ______ ___ _ ___
    • recombinant protein
    • hairy cell leukemia
    • hepatitis B & C
  15. ___________ is the primary hormone that regulates proliferation of committed progenitor (______) cells into
    _________
    • Erythropoietin
    • CFU-E
    • erythrocytes
  16. The gene for erythropoietin is present on ________ __ and expressed by the ______ _______ ________ cells
    • chromosome 7
    • renal peritubular interstitial
  17. ________ ______ has identical amino acid sequence to native erythropoietin
    Epoetin alfa
  18. Epoetin alfa is produced in ______ _______ _________ cells because this is a mammalian cell line that ____________ proteins and is well adapted to cell culture.
    • Chinese hamster ovary
    • glycosylates
  19. Tissue Plasminogen Activator is a _____ ________ that consists of 527 aa
    serine protease
  20. Tissue Plasminogen Activator first binds to ________ via ______ _______ at its _______ ________ and activates ______ ________
    • fibrin
    • lysine residues
    • amino terminus
    • bound plasminogen
  21. Tissue Plasminogen Activator contains several functional domains including a ______-_______ _______, _____ ________ and ________ that determine _________ ___ ___ ____________
    • fibronectin-finger domain
    • EGF domain
    • kringles
    • clearance from the blood.
  22. ____________ has identical amino acid sequence to native tissue plasminogen activator
    Alteplase
  23. Reteplase modifications
    • Missing fibronectin-finger domain
    • EGF domain
    • kringle-1
  24. Tenecteplase modifications
    aa103; threo → asparagine

    aa117; asparagine → glutamine

    aa296-299; lys-hist-arg-arg →ala-ala-ala-ala-
  25. Lanoteplase modifications
    • Missing fibronectin-finger domain
    • EGF domain
    • aa117 asparagine → glutamine
  26. The insulin monomers are associated in ___________ __________
    hexameric aggregates
  27. If one alters the amino acid sequence, it can diminish the tendency for __________ and therefore more rapid _______ and shorter __________
    • aggregation
    • onset
    • duration of action
  28. Lispro (Humalog) is identical to human insulin except at amino acids _____ and _____
    • B28 (Pro)
    • B29 (Lys)
  29. ___________ the amino acids in Lispro impairs assembly of the _________
    • reversing
    • heximer
  30. The long acting analog is _______ ________
    Insulin glargine
  31. In insulin aspart, the ____ _______ is replaced by _______ _____
    • B28 proline
    • aspartic acid
  32. In glulysine, the ___ ______ is replaced by _________ ____ and ___ _______ replaced by ________
    • B29 lysine
    • glutamic acid
    • B23 asparagine
    • lysine
  33. In insulin glargine, the ___ _______ residues added to the
    ________ ____________ and an _______ residue replaces the _______ at ______
    • two arginine
    • B-chain C-terminus
    • asparagine
    • glycine
    • A21
  34. Rapidly acting analogs are ______ and ______ __________
    • Glulysine
    • Insulin aspart

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