Describe the Dominant‐negative approach.
Dominant mutations in which the product of the mutant allele interferes with the function of the normal allele in the heterozygous state.A mutation whose gene product adversely affects the normal, wild-type gene product within the same cell. This usually occurs if the product can still interact with the same elements e the wild-type product but block some aspect of its function product.
DN is inhibitor that leads to lack of function (post translational interference), Binding is normal but you can't phosphorylate. Can have dominant ligand or receptor.functional knockout (not a real knockout)
Get 50% Aa, 25% aa, 25%
AAPKA R-subunit is usedto create a dominantnegative mutant