Bio265 Lab Midterm

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Titika
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140718
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Bio265 Lab Midterm
Updated:
2012-03-11 14:49:25
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Microbiology Lab Midterm Bio265
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Microbiology Lab Midterm
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  1. Bacillus and Clostridium are what types of microbes?
    Endospore formers.
  2. Mycobacterium is what kind of microbe?
    Acidfast.
  3. On what type of media does Escherichia coli grow on?
    On Mac Conkey's agar and Eosine Methylene Blue agar
  4. On what type of culture media does Staphylococcus aureus grow on?
    On Mannitol Salt agar
  5. What feature of Serratia marcescens is important?
    As it grows at various temperatures.
  6. Name this type of growth on an agar slant.
    Rhizoid
  7. Name this type of growth on an agar slant.
    Aborescent.
  8. Name this type of growth on an agar slant.
    Echinulate.
  9. Name this type of growth on an agar slant.
    Filiform.
  10. Name this Form of colony growth on an agar plate.
    Irregular.
  11. Name this Form of colony growth on an agar plate.
    Rhizoid.
  12. Name this Form of colony growth on an agar plate.
    Circular.
  13. Name this Elevation of colony growth on an agar plate.
    Umbonate.
  14. Name this Elevation of colony growth on an agar plate.
    Convex.
  15. Name this Elevation of colony growth on an agar plate.
    Raised.
  16. Name this Elevation of colony growth on an agar plate.
    Flat.
  17. Name this type of growth in broth media.
    Sediment.
  18. Name this type of growth in broth media.
    Pellicle
  19. Name this type of growth in broth media.
    Uniform fine turbidity.
  20. Name this type of growth in broth media.

    Flocculent growth.
  21. Name this Margin type of colony growth on an agar plate.
    Entire.
  22. Name this Margin type of colony growth on an agar plate.
    Serrate.
  23. Name this Margin type of colony growth on an agar plate.
    Filamentous.
  24. Name this Margin type of colony growth on an agar plate.
    Undulate.
  25. Name this Margin type of colony growth on an agar plate.
    Lobate.
  26. Name this type of growth in Gelatin liquefaction.
    Crateriform.
  27. Name this type of growth in Gelatin liquefaction.
    Saccate.
  28. Name this type of growth in Gelatin liquefaction.
    Infundibuliform.
  29. Name this type of growth in Gelatin liquefaction.
    Napiform.
  30. Name this type of growth in Gelatin liquefaction.
    Stratiform.
  31. Name this agar plate.
    Mannitol salt agar, bottom plate is sterile.
  32. Name this agar plate.
    Mac Conkey's agar.
  33. Name this agar plate.
    Blood agar.
  34. Name this agar plate.
    Eosine methylene blue agar. where Eschericheria coli turns green.
  35. Steps for Tube to Tube Aseptic Transfer.
    1) Label the tube. Hold both tubes in your left hand and the loop in your right (if your right handed) with the base of the tube in the palm of the hand. Photo pg. 8 lab book.

    2) Heat the loop at least 20 sec. to sterilize. Cool loop 10 sec.

    3) With the right hand (palm up) remove the caps from the tubes. DO NOT SET THE CAPS DOWN ON THE TABLE AT ANY TIME.

    4) Keeping the tubes at a 45degree angle, to reduce the chances that microbes can enter from the air. Place the loop first in the tube with the bacteria; touch the loop to the inside of the first tube (5 sec.) to further cool it.

    5) Then pick up the sample of bacteria and transfer it to the second tube. DO NOT PUT THE LOOP DOWN OR ALLOW IT TO TOUCH ANYTHING.

    6) Partially cap both test tubes (if caps are put on completely) microbes might splatter from the inoculating instrument).

    7) Heat the loop ate least 20 sec. You may now put down the loop and completely cap the test tubes.

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