Spectrophotometry

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Author:
HappyJedi7
ID:
140871
Filename:
Spectrophotometry
Updated:
2012-03-10 17:50:22
Tags:
Bio 244 lab
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Description:
Unit 9
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  1. The amount of light absorbance in a solution depends upon...
    ...the concentration of the compound and the ability of that compound to absorb that particular wavelength of light.
  2. Monochromatic light
    A beam of light of a single wavelength
  3. As the light beam (emergent beam) exits the solvent, it strikes...
    ... a photoelectric cell, which generates an electrical current whose strength is proportional to the insensity of the emergent beam.
  4. The spectrophotometer tells us...
    ... the amount of monochromatic light that is transmitted or absorbed [in a solution].
  5. Correlation factor
    • takes into account the amount of the transmitted light that may be absorbed by the solvent
    • "subtracts" the amount absorbed by the solvent by the use of a blank
    • the blank contains only the solvent
  6. A white light source is...
    ... focused through a prism or diffraction grating. the white light is separated into a spectrum of light, and then a wavelength is selected. It is focused through a slit and then passed through a solution.
  7. If the light is transmitted, then it...
    ...passes through the solvent. Some light may be absorbed.
  8. Incident beam
    selected light wavelength

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