Principle of Heat and HAc for Protein
All methods for detection of protein, primarily albumin, depend on the precipitation of the protein by chemicals and heat. The degree of turbidity produced is proportional to the quantity of protein present. The coagulated protein is compared with untreated urine, and a rough estimate of the amount of protein is obtained. As the tests depend on the turbidity in the urine solution, URINE MUST BE CLEAR BEFORE USE.