Total number of different tests available on the analyzer.
Total test repertoire
Analyzers that can perform multiple tests at any one time.
Random access
Sample reaction performed in individual compartments
Sample and rgt contained in seperate cuvettes
Runs multiple tests on multiple samples
Discrete Analyzers
Same test performed simultaneously on all samples
Batch analyzer
Continuosly pump liquids through system of continuous tubing
Samples introduced in sequential manner & each sample flows through same rxn path
Air bubbles introduced in line to seperate samples from reagents
Continuous Flow Analyzer
Uses force from centrifugation to transfer and contain liquids in seperate cuvets
Measurements taken @ perimeter of spinning rotor
Centrifugal analysis
Minumum time required to obtain result after initial sampling
Dwell Time
Maximum number of samples or tests that can be processed in an hour
Number of samples processed/hr times the number of tests performed on each specimen
Analyzer throughput
Time it takes from when the sample is collected until the results are reported.
Turn-around-time (TAT)
Accounts for 30-50% of testing in many labs
TAT(turn around time) < 1hr
STAT testing
Give some advantages of automation.
Increase number of tests performed
Minimizes variation in test results
Minimizes laboratory errors
expands test menu
reduces TAT
What are 4 laboratory processes used in automation?
test ordering
sample collection
sample transport
front-end processing
sample analysis
result aquisition
reportinf of results
archiving and sample disposal
Name the chemistry analyzer operations.
Sample identification
Determine test(s) to perform
Reagent systems and delivery
Specimen measurement and delivery
Chemical reaction phase
Measurement phase
Signal processing and data handling
Send result(s) to LIS
Name the chemistry analyzer operation based of the descrition:
This is usually done by reading a bar code. This information can also be entered in manually.
Process where majority of errors occur in lab
Sample identification
Name the chemistry analyzer operation based of the descrition:
The LIS communicates to the analyzer which tests(s) have been ordered.
Determine test(s) to perform
Name the chemistry analyzer operation based of the descrition:
A small aliquot of the sample is introduced into the reaction cuvet.
Specimen measurement and delivery
Name the chemistry analyzer operation based of the descrition:
The sample and reagents are are mixed and incubated.
Chemical reaction phase
Name the chemistry analyzer operation based of the descrition:
Optical reading may be initiated before or after all reagents have been added.
Measurement phase
Name the chemistry analyzer operation based of the descrition:
The analyte concentration is estimated from a calibration curve that is stored in the analyzer.
Signal processing and data handling
Name the chemistry analyzer operation based of the descrition:
The analyzer communicates results for the ordered tests to the LIS
Send result(s) to LIS
What are two methods used in mixing?
Stirring paddles
Rapid start stop action of rxn tray
Aspirates doulble volume of sample and pipets directly into 4 test channels @ one time
Double sample probes
Interfering substances in sample seperated out from rxn mixture
Seperation
Delay time to allow the reaction to take place, most reactions occur at 37o C
Incubation
rxn occurs for a predetermined amount of time
Reaction time
fully automated, random acces immunoassay analyzer
uses chemiluminescence
Avida Centaur
analog-to-digital convertor
converts voltage or current to a digital form that can be processed by computer
Computation
Allows results to be transferred directly from analyzer to laboratory computer system
Interface with LIS system
What 6 things need to be considered when choosing an automated analyzer?
Determine what type of analyzer is needed
Cost of analyzer
Calculate total cost of consumables & total cost/test
Determine mode of acquisition
Determine number full time employee's needed
Analytical capability of analyzer
What are disadvantages to automation?
Substantial financial investment
Cost can range between 1-3 million
Amount of open space
Volume of testing must justify initial costs
First fully automated clinical lab installed by Dr. Sasaki & colleagues
Japanese "black box" lab
Evoke an immune response, either cellular or humoral.
Contain one or more antigenic determinants (epitopes)
Antigens(immunogens)
Large proteins that bind specifically with Ag via F(ab) region
produced by B-lymphocytes
Antibodies
Attraction or force that causes to substances to unite
Between Ag & Ab