What is the temporal patter of expression of the following proteins important in T cell development: RAG-1, -2, TdT, pTα, CD3?
- 1. RAG-1, -2 - DN2->DN3 during β D:J and V:DJ recombination and DN4->DP during α chain recombination
- 2. TdT - from Stem cell through BP TCR with CD4:CD8 receptors ; N-nucleotide addition during rearrangement
- 3. pTα - Surrogate α chain; mostly during β recombination
- 4. CD3 - cell signaling throughout T cell development in thymus