Applications of DNA 1 (MJC)

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frozespot171
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144357
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Applications of DNA 1 (MJC)
Updated:
2012-03-29 23:29:00
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MJC Applications DNA
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Applications of DNA
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  1. Which description defines a cDNA library?

    1) a collection of clones overlapping DNA fragments representing the entire genome
    2) a collection of clones made by reverse transcription from mRNA
    3) a collection of clones whose relationship to each other can be established by chromosome mapping
    4) a collection of DNA segments from known chromosomal locations
    2) a collection of clones made by reverse transcription from mRNA
  2. _________ is used to cut the DNA at specific restriction sites while ________ is used catalyst the formation of phosphodiester bonds between adjacent nucleotides of a foreign gene and a vector to form recombinant DNA.

    1) DNA ligase, reverse transcriptase
    2) Restriction enzyme, DNA ligase
    3) DNA ligase, restriction enzymes
    4) Reverse transcriptase, restriction enzyme
    2) Restriction enzyme, DNA ligase
  3. Which of the following sequences in double-stranded DNA is most likely to be recognised as a cutting site for a restriction enzyme?

    1) AAGG
    TTCC
    2) AGTC
    TCAG
    3) GGCC
    CCGG
    4) AAAA
    TTTT
    • 3) GGCC
    • CCGG
  4. In recombinant DNA methods, the term vector can refer to

    1) the enzyme that cuts DNA into restriction fragments
    2) the sticky end of a DNA fragment
    3) a plasmid used to transfer DNA into a living cell
    4) a DNA probe used to identify a particular gene
    3) a plasmid used to transfer DNA into a living cell
  5. Which of the following cannot be used as a vector to tranform a bacterial cell?

    1) Phage
    2) Plasmid
    3) Bacterium
    4) All can be used as a vector
    3) Bacterium
  6. One important approach to gene cloning uses

    1) non-coding nucleotide sequences
    2) plasmids
    3) whole chromosomes
    4) viruses
    2) plasmids

  7. Which of the following is not needed for the process above?

    1) Restriction endonuclease
    2) Taq polymerase
    3) DNA ligase
    4) Vector plasmid
    2) Taq polymerase
  8. When bacteria are genetically engineered to produce human insulin,the ezymes used in the procedure are

    a ligase
    b restriction endonuclease
    c reverse transcriptase

    In which order are the enzymes used?
    First Last
    1) a c b
    2) b c a
    3) b a c
    4) c b a
    4) c b a
  9. A DNA library is

    1) a general collection of all genes sequenced thus far.
    2) DNA fragments inserted into a vector
    3) all DNA fragments identified with a probe
    4) a collection of DNA fragments that make up the entire genome of a particular organism
    4) a collection of DNA fragments that make up the entire genome of a particular organism
  10. In genetic engineering, products such as insulin can be artificially produced using a molecular template and specific enzymes.

    Which product cannot be formed in this way from the template?

    1) RNA from a DNA template
    2) DNA from a RNA template
    3) RNA from a polypeptide template
    4) polypeptide from a RNA template
    3) RNA from a polypeptide template
  11. Reverse transcription of an mRNA to synthesise cDNA for insertion into a vector does not involve the use of

    1) DNA primers
    2) ribonucleases
    3) DNA polymerase
    4) Taq polymerase
    4) Taq polymerase
  12. Vectors are small DNA molecules into which fragments of DNA can be inserted into.
    Which of the following is not a desirable characteristic of vectors?

    1) DNA molecules with a known structure
    2) Presence of genetic markers
    3) Contain a unique restriction site to a specific restriction enzyme
    4) Their replication cannot be controlled
    4) Their replication cannot be controlled
  13. The introduction of recombinant DNA into prokaryotic cells can be done through

    1) the use of a gene gun
    2) microinjection
    3) Agrobacterium-mediated transformation
    4) electroporation
    4) electroporation
  14. The formation of recombinant DNA can result in reannealed plasmids as well as foreign gene fragments that have reannealed. Hence there is need to identify which bacterial colony have taken up the recombinant.

    Which method cannot be used to identify which bacterial colonies have take up the recombinant DNA?

    1) Gene sequencing
    2) Gene expression
    3) Gene amplification
    4) Gene probe
    3) Gene amplification
  15. Which of the following statements about the differences between the genomic and cDNA library is true?

    1) A genomic library consist of the expressed portion of the genome only while a cDNA library contains the genetic information of the entire genome.
    2) A genomic library has introns while a cDNA library lacks introns.
    3) DNA polymerase is required to create a genomic library wile a cDNA library do not require DNA polymerase
    4) The reverse transcriptase method is used to generate a genomic library while a cDNA library is generated through the shot-gun method.
    2) A genomic library has introns while a cDNA library lacks introns.

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