Applications of DNA 2 (MJC)

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frozespot171
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144500
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Applications of DNA 2 (MJC)
Updated:
2012-03-29 23:27:33
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MJC Applications DNA
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Applications of DNA
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  1. Tissue culture is used to propagate plants using small plant parts.
    Which of the following is not an advantage to using tissue culture?

    1) Allows for rapid multiplication of plants
    2) Can be used on plant species that are difficult to propagate by tradition methods
    3) Not limited by season unlike tradition methods of propagation
    4) Results in plants gaining immunity to bacterial diseases
    4) Results in plants gaining immunity to bacterial diseases
  2. Which of the following is an implication of genetically modified organisms?

    1) Detrimental to the environment
    2) Concerns of that cloning techniques will be used on humans
    3) Exploitation of other species for gain
    4) All of the above
    4) All of the above
  3. In which ways are polymerase chain reaction different to DNA replication?

    i. DNA is heated to break the hydrogen bonds
    ii. DNA is unzipped
    iii. DNA primers are used
    iv. DNA polymerase enzymes are required

    1) i only
    2) i and iii only
    3) i,ii and iii only
    4) i,ii,iii and iv
    2) i and iii only
  4. PCR is a method to produce multiple copies of a template DNA molecule.

    How many molecules of DNA would there be after 30 PCR cycles?

    1) 230
    2) 231
    3) 302
    4) 303
    1) 230

  5. The graph above shows the cycles in PCR(Polymerase Chain Reaction).
    Which of the following is true?

    1) DNA replication occurs at A
    2) Denaturation of DNA strands occurs at B
    3) Primer extension occurs at C
    4) Annealing of primers occurs at A
    4) Annealing of primers occurs at A

  6. What occurs at stage X?

    1) Denaturation of DNA
    2) Annealing of primers
    3) DNA replication
    4) Primer extension
    1) Denaturation of DNA
  7. Nuclei acid gel electrophoresisis a technique of genetic engineering which separates nucleic acids on the basis of their rate of movement through a gel matrix in an electrical field.

    Which of the following is not needed in electrophoresis?
    1) Restriction enzymes
    2) Glycerol
    3) DNA ladder
    4) DNA ligase
    4) DNA ligase
  8. Nucleic acid gel electrophoresis has various applications such as

    1) DNA sequencing
    2) Detection of genetic disorders
    3) Analysis of PCR products
    4) All of the above
    4) All of the above
  9. Polymorphism is the variety of genotypes due to

    1) single nucleotide polymorphism
    2) insertion or deletion of nucleotides
    3) differences in lengths of repeated sequences
    4) all of the above
    4) all of the above
  10. Restriction Fragment Length Polymorphisms can be used as genetic markers for

    1) mapping of the human genome
    2) the identification of genetic disorders
    3) DNA fingerprinting
    4) All of the above
    4) All of the above
  11. DNA fingerprinting involves the use of a probe to identify a polymorphic region of DNA which cannot be used in

    1) identification of the culprit in crime investigations
    2) testing the paternity of a child
    3) determining phylogenetic relationship among different species
    4) the identification of non-mutated genes
    4) the identification of non-mutated genes
  12. During PCR, the reason why the denatured DNA strands anneal with primers and not each other is because

    1) the concentration of denatured DNA is much lower compared to the concentration of primers
    2) the primers are shorter and anneal more easily
    3) the primers only anneal to the 5' end of the denatured DNA strand
    4) the high temperature prevents the DNA from annealing together again
    1) the concentration of denatured DNA is much lower compared to the concentration of primers
  13. Which of the following are not limitations/disadvantages of the polymerase chain reaction?

    1) Can be easily contaminated
    2) Mutations may occur due to lack of proofreading activity
    3) Only short sequences of DNA can be amplified
    4) DNA degradation requires repeated heating
    4) DNA degradation requires repeated heating
  14. Which of the following enzyme is not used in human genome project?

    1) Taq polymerase
    2) DNA ligase
    3) Restriction enzymes
    4) RNA polymerase
    4) RNA polymerase
  15. Taq polymerase starts copying at

    1) RNA primers attached to the end of the desired gene
    2) The 5' end of the template DNA strand
    3) any free open end
    4) DNA primers attached to the end of the desired gene
    4) DNA primers attached to the end of the desired gene

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