rec exam 3.txt

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rec exam 3.txt
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  1. ´╗┐What is the main type of bacteria that we are dealing with for this lab?
    gram negative bacilli
  2. What is the first test that used to determine the different types of gram negative bacilli from each other?
    Lactose Fermentation
  3. A positive lactose fermentation test means that you have a gram negative bacilli that partakes in what kind of reactions?
    IMViC Reactions
  4. A negative lactose fermentation test means that you have a gram negative bacilli that partakes in what kind of reactions?
    TSI Reaction
  5. what does "IMViC " reactions stand for?
    ( Indole methyl red vogues-proskauer citrate )
  6. what does "TSI" reactions stand for?
    Triple sugar iron
  7. what is the result of the following IMViC Test? (I = +
    M=+
  8. what is the result of the following IMViC Test? (I = -
    M= -
  9. What is the result of the following TSI test? (Alkaline Slant (Red) Neutral Butt)
    Ps. aeruginosa
  10. What is the result of the following TSI test? (Acid Slant (yellow) Acid Butt (yellow))
    P. vulgaris
  11. What is the result of the following TSI test? (Alkaline Slant (Red) Acid butt (yellow))
    The need for a motility test (seeing if the tube is clear or cloudy)
  12. with a TSI result of
    Alkaline Slant (Red) and Acid butt (yellow) and a motility test of +
  13. with a TSI result of
    Alkaline Slant (Red) and Acid butt (yellow) and a motility test of -
  14. E coli's microscopic morphology
    bacillus (rod)
  15. E aerogenes microscopic morphology
    bacillus (rod)
  16. E coli's gram stain reaction
    negative (pink)
  17. E aerogenes gram stain reaction
    negative (pink)
  18. E coli's growth on a macConkey agar plate (6)
    Medium
  19. E aerogenes growth on a macConkey agar plate (7)
    Medium
  20. E coli indole test result
    "+" (pink-dark red)
  21. Ent aerogenes indole test result
    "-" (yellow)
  22. e coli methyl red test result
    "+" (distinct red color)
  23. ent aerogenes methyl red test result
    "-" (Yellow Color)
  24. E coli Voges-proskauer test result
    "-" (yellow/brown color)
  25. Ent aerogenes Voges-proskauer test result
    "+" (pink color)
  26. e coli citrate utilization test result
    "-" (no growth with no color change)
  27. ent. aerogenes citrate utilization test result
    "+" (growth with an intense blue color on slant)
  28. What are the only two bacteria that can result from the IMViC tests?
    E coli and ent aerogenes
  29. How do you preform the indole test?
    1)shake tubes of tryptone broth to mix culture 2) add 10 drops of kovac's reagent to tubes
  30. How do you preform the methyl red test ?
    1) shake tubes of M.R.-V.P Medium well to mix 2) 20 drops of the M.R-V.P Medium from each culture into a small tube. 3)small tube used for Voges-Proskauer Test 4)5 drops of methyl red to remaining tubes 5) read immediate reaction
  31. How do preform the Voges-Proskauer test?
    1) take the tubes w/ 20 drops M.R.-V.P Medium from the methyl red test
  32. A positive indole test
    pink to dark red color within 5 mins
  33. a negative indole test
    yellow color
  34. a positive methyl red test
    red color (distinct) (very acidic pH 4.5 or less)
  35. a negative methyl red test
    yellow color (less acidic pH 6.0 or more)
  36. A positive Voges-Proskauer test
    pink color
  37. A negative Voges-Proskauer test
    yellow/brown color
  38. a positive simmons citrate agar reaction
    growth with an intense blue color on the slant
  39. a negative simmons citrate agar reaction
    no growth w/ no change in color (green)
  40. Acid slant (yellow) + Acid butt (yellow) means what kind of fermentation?
    lactose and/or sucrose
  41. alkaline slant (red) + Acid Butt (yellow) means what kind of fermentation?
    Glucose
  42. alkaline slant (red) + Alkaline butt (red) means what kind of fermentation?
    No fermentation
  43. Production of H2S is indicated by what in the TSI test?
    black precipitate of ferrous sulfide in the medium
  44. In the motility medium a positive test is
    motile organisms : a diffuse growth from the stab line
  45. in the motility medium a negative test is
    non-motile organisms: growth restricted to stab line
  46. What is the purpose of the urea agar?
    observe for the presence of the enzyme urease.
  47. urea positive test
    change of agar to pink
  48. urea negative test
    NO change in agar color
  49. proteus vulgaris microscopic morphology
    bacillus
  50. proteus vulgaris gram stain reaction
    negative
  51. proteus vulgaris motility
    "+" motile
  52. proteus vulgaris colony morphology
    "swarming effect" concentrated circle of growth
  53. proteus vulgaris pigment production
    Slightly yellow
  54. proteus vulgaris odor
    vulgar
  55. proteus vulgaris reaction on MacConkey Agar
    Negative
  56. proteus vulgaris growth in trypticase soy broth
    "+"
  57. proteus vulgaris urease production
    "+"
  58. proteus vulgaris triple sugar iron agar slant
    Acid "yellow"
  59. proteus vulgaris triple sugar iron agar butt
    Acid "Yellow"
  60. proteus vulgaris triple sugar iron agar gas
    +
  61. proteus vulgaris triple sugar iron agar H2S
    "+" (black preci.)
  62. pseudomonas aeruginosa microscopic morphology
    bacillus
  63. pseudomonas aeruginosa gram stain reaction
    negative
  64. pseudomonas aeruginosa motility
    + (strict aerobe
  65. pseudomonas aeruginosa colony morphology
    small to medium
  66. pseudomonas aeruginosa pigment production
    greenish
  67. pseudomonas aeruginosa odor
    "grape-like" sick-sweet smell
  68. pseudomonas aeruginosa reaction on MacConkey Agar
    negative
  69. pseudomonas aeruginosa growth in trypticase soy broth
    + (heavy surface growth = pellicle
  70. pseudomonas aeruginosa urease production
    "-"
  71. pseudomonas aeruginosa triple sugar agar slant
    alkaline "red"
  72. pseudomonas aeruginosa triple sugar agar butt
    No change
  73. pseudomonas aeruginosa triple sugar agar gas
    No change
  74. pseudomonas aeruginosa triple sugar agar H2S
    "-" strict aerobe
  75. S. paratyphosa microscopic morphology
    Bacillus
  76. S. paratyphosa gram stain
    gram negative
  77. S. paratyphosa motility
    +
  78. S. paratyphosa colony morphology
    large
  79. S. paratyphosa fermentation reaction on MacConkey Agar Plate
    "-"
  80. S. paratyphosa triple sugar iron agar slant
    Alkaline (red)
  81. S. paratyphosa triple sugar iron agar butt
    Acid (yellow)
  82. S. paratyphosa triple sugar iron agar gas
    "+"
  83. S. paratyphosa triple sugar iron agar H2S
    "-"
  84. S. typhimurium microscopic morphology
    Bacillus
  85. S. typhimurium gram stain
    negative
  86. S. typhimurium motility
    "+"
  87. S. typhimurium colony morphology
    large
  88. S. typhimurium fermentation reaction on MacConkey Agar Plate
    "-"
  89. S. typhimurium triple sugar iron agar slant
    Alkaline (red)
  90. S. typhimurium triple sugar iron agar butt
    Acid (yellow)
  91. S. typhimurium triple sugar iron agar Gas
    "+"
  92. S. typhimurium triple sugar iron agar H2S
    "+"
  93. sh. dysenteriae microscopic morphology
    bacillus
  94. sh. dysenteriae gram's stain
    Gram negative
  95. sh. dysenteriae motility
    "-"
  96. sh. dysenteriae colony morphology
    large
  97. sh. dysenteriae fermentation reaction on MacConkey Agar Plate
    "-"
  98. sh. dysenteriae triple sugar iron agar slant
    alkaline (red)
  99. sh. dysenteriae triple sugar iron agar butt
    Acid (yellow)
  100. sh. dysenteriae triple sugar iron agar Gas
    "-"
  101. sh. dysenteriae triple sugar iron agar H2S
    "-"

  102. The horizontal area underneath the clear zone in the bacterial lawn is called the...
    Diameter of zone of inhibition

  103. In a disk method, the bulk of the bacterial growth is in.....
    the contiguous lawn

  104. The clear zone in the bacterial lawn is called the....
    inhibition of bacterial growth
  105. The drug that gives us the largest zone of inhibition will be the....
    drug of choice
  106. ´╗┐penicillin disk code
    P-10
  107. penicillin disc potency
    10 U
  108. streptomycin disk code
    S-10
  109. streptomycin disc potency
    10ug
  110. choramphenicol disk code
    C-30
  111. Choramphenicol disc potency
    30ug
  112. Ampicillin disk code
    AM-10
  113. ampicillin disc potency
    10ug
  114. tetracycline disk code
    Te-30
  115. tetracycline disc potency
    30ug
  116. Cephalothin disk code
    CF-30
  117. Cephalothin disc potency
    30ug
  118. Colistin disk code
    CL-10
  119. Colistin disc potency
    10ug
  120. methicillin disk code
    DP-5
  121. methicillin disc potency
    5ug
  122. what is the turbidity of McFarlands?
    0.5 standard = (1.5 x 10^8 bacteria per ml)
  123. MIC stands for
    minimal inhibitory concentration
  124. MLC stands for
    minimal lethal concentration.
  125. MIC = MLC that means the organism was
    bactericidal
  126. MIC not equal to MLC means that the organisms
    is bacteriostatic
  127. Compartment 1 enterotube contents
    glucose and gas production
  128. Compartment 2 enterotube contents
    lysine
  129. Compartment 3 enterotube contents
    ornithine (ORN)
  130. Compartment 4 enterotube contents
    h2s and indole
  131. Compartment 5 enterotube contents
    adonitol
  132. Compartment 6 enterotube contents
    lactose
  133. Compartment 7 enterotube contents
    arabinose
  134. Compartment 8 enterotube contents
    sorbitol
  135. Compartment 9 enterotube contents
    Voges- Proskauer
  136. Compartment 10 enterotube contents
    dulcitol
  137. Compartment 11 enterotube contents
    urea
  138. Compartment 12 enterotube contents
    citrate
  139. what are the incubation time and temps for the enterotube?
    35 to 37 degrees C for 18-24 hours
  140. what compartments in the enterotube gets additional holes poked thru them to promote aerobic growth?
    the last 8
  141. The MIC is the lowest concentration of...
    the antimicrobic that causes complete inhibition of bacterial growth (inhibition can be bacteriostatic or bactericidal)
  142. the MLC is the lowest concentration of...
    antimicrobic which prevents growth when the organism is sub cultured in the absence of the antimicrobic.
  143. bacteriostatic
    inhibits bacterial growth
  144. bactericidal
    preventing infection by inhibiting the growth or action of microorganisms

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