Vector: carriers recombinant DNA into a host cell
Plasmid: a small fragment of extrachromosal DNA, usually circular, that replicates independently of the main chromosome.
- 1.) Origin of replication site
- 2.) Marger gene (antibiotic resistant)
- 3.) Promoter / Terminator regions
- -Cut plasmid w the same restriciton endonuclease
- -Ligate to create recombinant plasmid