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With the exception of identical twins, the possibility of two people having the same DNA composition is in the order of 1 in 10 to the ____ power.
Restriction Fragment Length Polymorphism (RFLP) is a DNA analysis that cuts DNA into pieces using a _______ enzyme, which cuts DNA when it recognizes the GGCC/CCGG sequence. The resultant lengths or fragments depend on the locaiton of those sequences and are called RFLP's. The analysis is accomplished with the _________ ___ process
Limitations to RFLP includes the fact that it requires a ________ sample, takes a lot of _______ (4 wks to 6 mos), and once the sample is used, it is gone.
_________ or __________ __________ _________ are usually 3 to 5 base pairs in length. It is the ________ number of these repeats that distinguishes one profile from another and forms the basis of _______ analysis.
- Short Tandem Repeats
Statistical estimates suggest that typeing information from ____ different STR markers will afford virtual identification of an individual. The CODIS (___________ ______ _______ _______) is an FBI program supporting a network of local, state and national databases housing DNA samples of convicted offenders and crime scene samples. These labs are required to use ____ core STR loci technology. The ____ marker in PCR STR DNA is a ______ identifier.
- Combined DNA Index System
When collecting suspected blood in snow or water, do so immediately to prevent further __________. Eliminate as much snow as possible. Place in a clean airtight container. _________ the evidence and submit as soon as possible to the lab.
For blood examination, the FBI submission requirements include a request letter containing the following:
(1) a brief _________ of facts relating to the case
(2) _____ made by suspect regarding the source of the blood
(3) info about whether ___ blood is present or suspected
(4) info about whether stains were _________ or diluted with other body fluids
(5) info about the health of the vic and suspect, suchas as _____, ______, or _____
- AIDS, Hep, TB
Semen evidence from a sexual assault victim should be ________ and submitted to the lab ASAP.
Hair should be picked up carfully with clan forceps to prevent dammagin the root tissue; __________ hair mixed with suspected body fluids; package ea. group of hair ____ in clean paper or enevelope with sealed corners (no plastic); and __________ and submit ASAP.
When collecting red skeletal muscle for DNA processing, you should collect 1-2 cubic inches, place in a clean airtight ______ container ___________ formalin or formaldehyde.
When collecting long bone for DNA processing, you should
collect 3-5 inches, place in clean _______ or and envelope with sealed corners. ___________ the evidence, place in styrofoam containers adn ship overnight on dry ice.
Collect teeth in the following order: unrestored molar, premolar, ______, front tooth; then restored ______, premolar, canine and ________. Package as bone, place in styrofoam containers and ship overnight on dry ice.
In bodies involving advaced state of decomposition, _______ becomes the best source of DNA.
CODIS has three levels : ________, or the national level; _____, the local level; ________ the state level.
Two indexes within CODIS--the_______ index and the ___________ index.
- forensic (crime scene)
Sample collected at scene --> PCR DNA analysis--> entered locally into ____--> run against forensic index--> run in offender index