Genetics Final exam

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Serenity90
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148211
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Genetics Final exam
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2012-04-17 21:20:44
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  1. Ioninzing forms of radiation such x-rays, cause mutations by
    creating ions pairs that lead to abnormal chemical reactions
  2. A collection of clones that contains at least one copy of every DNA molecules that was synthesized from mRNA is called a (an)
    Complementary DNA library
  3. The reason that a vector must carry a gene that provides antibiotic resistance to its host is so that it can
    allow the selection of the host cells that have taken up the vector and its rcDNA.
  4. The reason that a vector is engineered to contain unique restriction sites is to
    allow the vector and the DNA that will be inserted to be cut in an identical manner.
  5. The reasonb that a vector must contain the Ori gene is to
    allow the DNA that is inserted to be replicated
  6. an enzyme that recognizes a specific base sequence in DNA and makes a nonspecific cut justt outside of that sequence
    restriction DNA endonucleases type I
  7. If a protein is going to be secreted from the cell after translation, which of the following is likely to be a compenent of its newly synthesized polypeptide chain?
    answers?
  8. (in the previous answer) the molecule ___ binds to a molecule called the___
    Answe?
  9. Name the vector best suited to carry a 40kb segment ot rcDNA
    a cosmid
  10. a filtration column that contains beads that have poly-T sections attached is best used for
    collecting eukaryotic mRNA molecules
  11. Which of the following vectors inserts recombinant DNA into host cells by transduction
    Phage Lambda
  12. Which of the following vectors insert recombinant DNA into host cells by transformation?
    pUC18
  13. If we wanted to synthesizes DNA and mRNA that we collected, we would FIRST add
    poly-T primer
  14. If we wanted to synthesizes DNA and mRNA that we collected, we would SECOND add
    RNA-dep. DNA polymerase
  15. The pUC-19 vector was engineered so that the vectors that do not incorporate the rcDNA insert will still have a functional lac Z+ gene that will produce blue colonies in E.coli transformats; however, vectors that do incorporate the rcDNA insert will not have a functional lac Z+ gene, producing transformant that will
    Allows the selection of the host cells that have taken up rcDNA plasmids.
  16. The photoactivated repair system works by
  17. The excision repair system works by
    Using DNA endonuclease, polymerase and ligase activity to cut out the thymine-Thymine dimers and replace the damaged DNA.
  18. They type of mutation that results in uncommon base pairing caused by hydrogen atom moving to an uncommon (rare state) position in the same nitrogen base, is call a (an)
    Tautomeric Shift
  19. The loss of a nucleotide from a gene may result in change in th e rading of its base sequence , for example CATCATCAT_ATCATCACTACT. This type of mutation is called a (an)
    frame shift
  20. Non-ionizing forms of radiation such as ultraviolet light, causes mutations by
    energizing the electrons of bases such that they form abnormal chemical bonds.

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