BIO 371 E2 LAB 8

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shockwave
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148944
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BIO 371 E2 LAB 8
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2012-04-21 00:28:10
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BIO 371 E2 LAB
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BIO CELL 371 E2 LAB 8 GSU 2012
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  1. PLASMA MEMBRANE THICKNESS?
    7.5 nm
  2. DEFINE OVERTON'S RULE
    • 1899
    • demonstrated that the greater the lipid solubility of a compound, the greater its rate of penetration.
  3. who proposed the fluid mosaic model?
    singer and Nicholson 1972
  4. INTEGRAL PROTEIN ARE ______, HAVING _______ ____ ATTACHED TO THEM.
    • GLYCOPROTEINS
    • CHARBOHYDRATE CHAINS
  5. WATER AND CELLS IN A TUBE.
    WHAT IS THE SOLVANT?
    WHAT IS THE SOLUTE?
    • WATER IS THE SOLVENT
    • PROTEINS, SUGARS ARE THE SOLUTE
  6. DEFINE SOLUTE CONCENTRATION
    the total number of dissolved particles per unit of volume solution
  7. IF THE SOLUTE CONCENTRATION OUTSIDE THE CELL IS_______ then the inside_THE EXTERNAL SOLUTION IS DESCRIBED AS_____.
    hypertonic
  8. if a leaf is placed in a hypertonic sucrose solution what is the result
    water will leave the cell by osmosis,plasmolysis will occur.
  9. when a RBC is placed in a hypertonic salt solution what occurs?
    Rbc's (erythrocytes) loses water, and shrinkage or cremation occurs.
  10. True or false sodium fluoride penetrates human urethra sites so slowly that the rate can be considered negligible.
    True
  11. in a___________ salt solution, water enters the RBC. A volume increase of over 67% will cause what?
    • Hypotonic
    • hemolysis
  12. _______ generally penetrate by________ transport, where the driving force is the concentration gradient across the membrane.
    • Non-electrolytes
    • passive
  13. generally the rate of penetration increases with what?
    • Increasing lipid solubility of the permanents.
    • molecular structure of the permanents is another significant factor in determining its rate of penetration. (Hydroxyl groups form hydrogen bonds with the water molecules).
    • And the molecule size
  14. A frequently used measure of lipid solubility is what?
    • Easter/water partition coefficient.
    • (Solubility in diethyl ether)/(solubility in water)
    • remember that this also includes alcohols.
  15. in the active transport study with the cells the substance to be transported is neutral red, a dye that is ______ in acidic solutions and______ in basic solutions.
    • RED......ACIDIC
    • YELLOW...BASIC
  16. TRUE OR FALSE
    conditions inside living yeast cells are relatively acidic?
    True
  17. in the concentrations of sucrose is plasmolysis not observed? Observed?
    • Not observed at .2 M
    • observed at .6 M
  18. DEFINE TONOPLAST
    • The cytoplasmic membrane surrounding the vacuole, separating the vacuolar contents from the cytoplasmin a cell.
  19. WHEN YOU ADD THE .6M SUCROSE SOLUTION TO THE ELODEA LEAF WHAT HAPPENED AND WHY?
    • DEPLASMOLYSIS.
    • THE SUCROSE SOLUTION WAS HYPERTONIC TO THE LEAF. (HYPERTONIC SOLUTION). IT WAS EXTENSIVE.
    • INSIDE OF LEAF IS.3M SUCROSE NATURALLY.

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