Micro Lab

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Author:
jfellings
ID:
14956
Filename:
Micro Lab
Updated:
2010-04-17 19:00:30
Tags:
microbiology
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Description:
Micro Lab Final
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  1. Basic Dye
    Methylene Blue, Safranin, Crystal Violet
  2. Acidic Dye

    What does it stain?
    Eosin, Congo Red, Nigrosine

    Used to stain Background
  3. Gram Stain

    Order?
    • Crystal Violet
    • Iodine,
    • Alcohol
    • Safranin
  4. Capsul Stain
    Stains bacteria with capsules

    Use Congo Red or Crystal Violet
  5. Endospore Stain

    What organism does it test for?
    Stains spores with Malachite Green

    Tests for Bacillus Clostridium

    Spores are green, vegetative cells are pink
  6. Motility Test

    What Media?

    Organisms?
    Mot-S or Sim tube

    Flagellates or other motile organisms

    Cloudiness is a positive test.
  7. Sulfur Utilization?
    Media?
    Organisms?

    • Black is positive result
    • SIM Tube or KIA
    • Proteus Vulgaris
  8. Aerobe vs Anaerobe?

    Media?
    Use a Thioglycolate tube.

    Aerobes on surface, anaerobes grow through medium. Faculitive Anaerobes near surface
  9. Antimicrobial/ Antibiotic Sensitivity

    Medium?
    Kirby Bauer Method on Mueller Hinton Agar plate
  10. Oxidase

    Organisms?
    Medium?
    Positive Result?
    • + Neisseria
    • + Pseudomonas
    • - Enterobacteria

    • TSA Plate
    • Pink, Maroon color. Turns blue after 60 sec
  11. Phenylalanine Deamination

    Organisms?
    Medium
    Positive Result
    • Proteus
    • Providentia
    • Morganella

    Slant Tube

    Green on slant
  12. Staphylococci Identification

    Tests
    • Catalase: Staph Aureus is +
    • MSA: Yellow= aureus
    • Blood Agar: Clear Zone is Aureus
  13. Streptococci Identification
    Tests
    • Blood Agar:
    • Bacitracin A=pyogenes, CAMP=agalactiae, P=Pneumonia
  14. Hemolysis
    Beta
    Alpha
    Gamma
    • Beta: Clear zone
    • Alpha: Green Zone
    • Gamma: No clear zone
  15. Starch Hydrolysis
    Organism
    Medium
    • Bacillus subtilis
    • Starch agar plate
    • Clear zones is positive result
  16. Tryptophan
    Organism
    Media
    Reagent
    Positive Result
    • Citrobacter, some Shigella
    • SIM
    • Kovac's Reagent
    • Red Layer on top
  17. Urea Hydrolysis
    Organism
    Media
    Positive
    • Proteus Providencia Morganella
    • Urea Broth (Phenol Red in media)
    • Red color
  18. Yogurt Production
    Organism
    Strep Thermophilus
  19. Lactose Positive Organisms
    • Escheracia
    • Enterobacter
    • Klebsia
    • Citrobacter
  20. Total Magnification
    Ocular x Objective
  21. Limit of Resolution
    Minimum distance between two objects that are discernable to see them both
  22. Acid Stains have what charge?
    Basic Stains have what charge?
    Bacterial Cell walls have what charge?
    • Negative Charge
    • Positive Charge
    • Negative Charge
  23. Differential Stain Purpose
    Example
    • Two different stains
    • Gram stain or Acid fast stain
  24. Flagella
    Monotrichous
    Lophotrichous
    Amphitrichous
    Peritrichous
    • M: Single Flagella
    • L: Many on one side
    • A: One on each side
    • P: Many all over
  25. Catalase
    Organisms
    Results
    • + Staphylococcus
    • - Enterococcus
    • -Strep
  26. Enterics
    Gram?
    Oxidase
    Glucose
    Nitrite
    Examples
    • Gram negative
    • Oxidase Negative
    • Glucose Positive
    • Nitrate Positive
    • Examples: E. Coli, Klebsia, Enterobacter, Salmonella, Shigella
  27. Psuedemonas
    Oxidase
    Motility
    Fermentation
    • Oxidase Positive
    • Motile
    • Fermentation Negative
  28. 
    • Candida Albicans
    • (Yeast)
  29. Aspergillis
  30. Penicillum
  31. Plasmodium
  32. Differential Media
    Examples
    Some metabolic characteristic of an organism is detected due to media

    KIA, MaConkie Agar, Urea, Starch Hydrolysis
  33. Selective Media
    Allows the growth of certain organisms and inhibits others

    MSA, Bile Eschulen
  34. Enriched Media
    Rich in nutrients

    Blood Agar, Chocolate Agar,
  35. Lactose Fermenter
    All Coliforms
  36. Countable Plates
    20-80 coliform colonies
  37. Protozoa
    • Amoebas:Entamoeba
    • Ciliates: Balantidium
    • Flagellates: Giardia, Trichomonas
  38. Platyhelminthes
    • Fasciola hepatica
    • Shistosoma
  39. Nematodes
    • Enterbius
    • Ascaris
  40. Bacillus
    Gram
    Morphology
    Media
    Pos Results
    • Positive
    • Rod
    • TSA Catalase +
    • Starch Digestion
    • H2O2 and Gas Bubbles
  41. Citrobacter
    Gram
    Morphology
    Media
    Positive Results
    • Gram Negative
    • Morphology: Rod
    • Media:
    • MAC- Red or pink colonies
    • EMB- same as above
    • KIA -A/A++
    • H2S +
    • Lactose+
    • Glucose +
    • Oxidase -

    Urea-No growth
  42. Clostridium
    Gram
    Morphology
    Media
    Positive Results
    • Gram Positive
    • Morphology Rod
    • Media:
    • TSA-growth
    • Blood Agar-hemolysis
    • Catalase -
  43. Micrococcus luteus
    Gram
    Morphology
    Media
    • Gram Positive
    • coccus
    • TSA-Yellow colonies
  44. Neiseria
    Gram
    Morphology
    Media
    • Gram Negative
    • Diplococcus
    • Thayer Martin
    • Chocolate Agar
    • Oxidase positive
    • purple color change
  45. Proteus Vulgaris
    Gram
    Morphology
    Media
    • Gram Negative
    • Rod
    • MAC/EMB-colorless
    • KIA -black
    • Urea Red
    • Lactose -
    • Glucose +
    • Oxidase -
  46. Pseudomonas
    Gram
    Morphology
    Media
    • Gram Negative Rod
    • KIA-Alk/Alk. - -
    • TSA -green pigment
    • Glucose -
    • oxidase +
  47. Salmonella
    Gram
    Morphology
    Media
    • Gram Negative rod
    • KIA-Alk/Acid +- Black
    • Urea -
    • Lactose -
    • Glucose +
    • Oxidase -
  48. Serratia
    Gram
    Morphology
    Media
    • Gram Negative Rod
    • KIA-Acid/Acid --
    • TSA 25 degree -red pigment
  49. Staph Aureus
    Gram
    Morphology
    Media
    • Gram Positive Rod
    • Blood Agar: Beta Hemolysis
    • MSA: Yellow media
    • Catalase +
  50. Staph epidermis
    Gram
    Morphology
    Media
    • Gram Positive Coccus
    • Blood Agar: Gamma Hemolysis
    • MSA: Pink media and colonies
    • Catalase +
  51. Strep feacalis
    Gram
    Morphology
    Media
    • Gram Positive Coccus
    • Blood agar: alpha hemolysis
    • Bile Eschulen: more than 1/2 slant is black +
    • catalase -
  52. Strep Pyogenes
    Gram
    Morphology
    Media
    • Gram positive Coccus
    • Blood agar: beta hemolysis, bacitracin sensitive
    • Bile Esculin -
    • catalase-
  53. E. Coli
    Gram
    Morphology
    Media
    • Gram Negative Rod
    • EMB: metallic green
    • MAC: pinka
    • H2S:-
    • Lactose +
    • Glucose+
    • Oxidase -
  54. Enterobacter
    Gram
    Morphology
    Media
    • Gram Negative Rod
    • EMB: Color
    • Mac: Color
    • Motility+
    • Indole -
    • Lactose +
    • Glucose +
    • Oxidase -
  55. Klebsia
    Gram
    Morphology
    Media
    • Gram Negative Rod
    • EMB: Color
    • MAC: Mucoid
  56. Shigella
    Gram
    Morphology
    Media
    • Gram Negative Rod
    • EMB: Colorless
    • MAC Colorless

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