Micro lab

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Anonymous
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150415
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Micro lab
Updated:
2012-04-26 23:53:11
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lab final
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lab final
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  1. Phenylethyl alcohol agar
    • inhibitor - phenylethyl alcohol
    • inhibits gram (-) growth by breaking down membrane
    • selective
  2. Columbia CNA w/5% sheep blood
    • inhibitor - colistin and nalidixic acid
    • inhibits gram (-) by affecting membrane integrity and interfering with DNA replication
    • indicator - blood
    • both
    • positve - good growth with clearing or greening
  3. Bile esculin agar
    • inhibitor - bile salts
    • separates the streptococcu group and enterococci from othe streptococci
    • indicator - ferric citrate
    • both
    • positive - darkened media
  4. Mannitol salt agar
    • inhibitor - sodium chloride
    • dehydrates and kills most bacteria
    • indicator - phenol red
    • both
    • positive - bright yellow colonies with yellow halo
    • can also tell the pH of the organism
  5. MacConkey agar
    • inhibitors - bile salts & crystal violet
    • inhibits gram (+)
    • indicator - phenol red
    • both
  6. Eosin Methylene Blue agar
    • inhibitor - lactose & sucrose
    • inhibit gram (+)
    • indicator - neutral red dye
    • both
    • positive - pink or dark growth
  7. Hecktoen enteric agar
    • inhibitor - bile salts
    • inhibit gram (+) and enterics
    • indicator - ferric ammonium citrate, bromothymol blue, acid fuchsin
    • both
    • sulfer reduction is indicated by blue/green growth with black precipitate
  8. Xylose lysine desoxycholate agar
    • inhibitor - desoxycholate
    • inhibits gram (+)
    • indicator - phenol red
    • both
  9. oxidation-fermentation test
    • differentiate bacteria based on their ability to oxidize or ferment sugars
    • stab inoculated then sealed with oil
    • 2 tubes
    • pos - yellow
    • neg - green or blue
  10. phenol red broth
    • fermentation with acid and/or gas
    • 4 tubes
    • broth inoculated
    • pos - yellow
    • neg - red
  11. methyl red and VP test
    • methyl red - detect if organisms capable of performing a mixed acid fermentation
    • VP - organisms are capable of fermenting glucose
    • 3 tubes
    • broth inoculate
    • add reagents
    • pos - red
    • neg - no color change
  12. catalase test
    • identify organisms that produce catalse
    • one
    • slant streak
    • add hydrogen peroxide
    • pos - bubbles
    • neg - no bubble
  13. oxidase test
    • identify bacteria containing oxidase
    • wooden end of sterile cotton applicator
    • reagent slide
    • pos - dark blue
    • neg - no color change
  14. nitrate reduction test
    • reduction of nitrate to nitrite
    • 1 tube
    • broth inoculate
    • add 8 drops of reagent A and B and zinc dust
    • pos - gas/ red after reagents
    • neg - red color after zinc
  15. citrate test
    • determine the ability of an organism to use citrate as its sole source of carbon
    • 1 tube
    • light inoculum streak with needle
    • pos - blue or no color change with growth
    • neg - no color change or growth
  16. Malonate test
    • ferment or utilize malonate
    • 1 tube
    • broth inoculate
    • pos - dark blue
    • neg - no color change
  17. decarboxylase test
    • if the organism produces decarboxylase enzyme
    • 4 tubes
    • streak
    • add mineral oil
    • pos - purple color
    • neg - no color/yellow
  18. Phenylalanine deaminase test
    • if the organism contains phenylalanine deaminase
    • 1 tube
    • streak with heavy inoculum
    • add drops of ferric chloride after incubation
    • pos - green
    • neg - no color change
  19. Starch hydrolysis test
    • if amalyse is or isnt present
    • one plate for all
    • spot-inoculated
    • add iodine after
    • pos - clearing around growth
    • neg - no clearing
  20. Urea hydrolysis
    • differentiates rapid urease (+) bacteria from slower urease (+) and urease (-) bacteria
    • one tube and one plate
    • heavy broth inoculate and streak inoculate
    • read 24 and 48 hours - re incubate (-)
    • pos - pink
    • neg - orange/yellow
  21. Gelatin hydrolysis test
    • ability to produce gelatinases
    • one tube
    • heavy stab-inoculate
    • reicubate if liquid
    • pos- liquid
    • neg - solid
  22. DNA hydrolysis test
    • ability to produce DNase
    • one plate for all
    • spot-inoculate
    • pos - clearing around growth
    • neg - no clearing
  23. SIM medium
    • identify bacteria capable of producing indole, usign the enzyme tryptophanase
    • combination - sulfer reduction and motility
    • one tube
    • stab-inoculate with needle
    • add kovacs' reagent after incubation
    • pos - red in alcohol layer of reagent, black in medium, growth radiating out
    • neg - reagent color unchanged, no black, no radiating growth
  24. Triple sugar iron agar
    • differntiate bacteria ont he basis of glucose fermentaiton, lactose fermentation, sucrose fermentation, and sulfur reduction
    • combination - animal proteins as sources of carbon and nitrogen, both ferrous sulfate and sodium thiosulfate as sources of oxidized sulfur
    • one slant
    • heavy streak, stab the butt
    • pos - yellow or red, black
    • neg - no color, no precipitate
  25. Lysine iron agar
    • differentiate enterics based on their ability to decarboxylate or deaminate lysine and produce hydrogen sulfide
    • combination - triple sugar iron agar
    • one slant
    • stab the agar and streak the slant
    • pos - purple/ red, black precipitate
    • neg - yellow
  26. Blood agar
    • isolationa nd cultivation of many types of fastidious bacteria
    • one plate
    • quadrant streak
    • pos - clearing or greening
    • neg - no change
  27. coagulase test
    • presence of either bound or free coagulase enzymes
    • one tube
    • broth inocualtion
    • mix tubes
    • pos - clumping, solid media
    • neg - no clumping, liquid
  28. motility test
    • motility
    • one tube
    • stabe inocualte
    • pos - radiatiing growth
    • neg - no radiating growth
  29. thiogllycoate medium
    • promote growht of a wide variety of fastidious microorganisms
    • one tube
    • broth inoculate with gentle mixing
    • pos results only
    • -obligate aerobic - top only
    • -obligate anaerobic - bottom only
    • -facultative bacteria - all throughout but more at top
    • -microaerophile - top and a little below
    • aerotolerant - all evenly through out

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