recombination conjugation transormation transduction

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  1. recombination
    • eukaryotic occurs during meosis
    • prokaryotic occurs when dna is transfered from donor to recepient cell
  2. recombination steps
    • donor dna alligns with recepient cell
    • bases align and RecA catalyzes strands
    • recipient chromosome contains new DNA
  3. cojugation
    • requires direct cell to cell contact and opposite mating types
    • donor cells carry plasmids
    • recipient cells dont
  4. sex pilli
    • for gram - cells
    • projections from the donor cell to get in direct contact with recipient cell
    • for gram + cells
    • produce sticky molecules to bring cells into direct contact
  5. high frequency conjugation
    f+ cell (donor) - recombiation with chromosome produces a Hfr cell
  6. low frequency conjugation
    f+ cell transfer plasmid to f- cell which results in a f+ cell
  7. transformation
    genes are transfered from one bacterium to another as naked DNA in a solution
  8. transformation steps
    • recipient cell takes up donor DNA
    • bases align
    • recombination occurs between donor and recipient DNA
    • outcome is a genetically transformed cell
  9. transduction
    bacteria DNA is transferred from a donor cell to a recipeint cell inside a virus that infects bacteria; bacteriophage
  10. transduction steps
    • phage infect donor bacteria cell
    • chromosome broken to peices so assembly of some bacterial DNAare packed and released after death of cell
    • bacterial DNA phage infects new host cell
    • recombination produces normally
  11. constitute
    gene always turned on
  12. represssion
    gene normall turned on but can be turned off in response to metabolic signal
  13. induction
    gene is normally turned off but can be turned on in response to metabolic signal
  14. repressors
    regulatory mechanism that inhibits gene expression and decreases enzyme sysnthesis
Card Set
recombination conjugation transormation transduction
test 3
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