Lab cards.txt

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Lab cards.txt
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2012-05-05 14:11:00
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Micro lab Exam II
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  1. Hydrolysis of starch show positive when
    Reagent iodine is added and there is no color change. Around streak
  2. Hydrolysis of starch is negative when
    Iodine is added and a dark purple color around streak line
  3. In hydrolysis of starch the coenzyme that promotes hydrolysis of starch into sugar is
    Amylase
  4. Starch is a
    Polysaccharide
  5. When doing a hydrolysis test on starch you are looking for
    Sugar
  6. When doing a hydrolysis of starch Bacillus spizizenii has the coenzyme
    Amylase
  7. When doing a hydrolysis of starch E. Coli has a negative or positive coenzyme of amylase
    Negative
  8. When doing a Hydrolysis of gelatin the coenzyme that promotes the hydrolysis in gelatin and make it liquefied
    Gelatinase
  9. When doing a hydrolysis on gelatin you are looking for the presence of
    Amino acids
  10. Hydrolysis of gelatin is negative when
    The gelatin remains solid
  11. Hydrolysis of gelatin is positive when
    Gelatin is liquid
  12. In hydrolysis of gelatin bacillus spizizenii has the coenzyme
    Gelatinase
  13. Gelatin is a
    Protein polymer
  14. Amino acids are a
    Protein monomer
  15. SIMS refers to
    Sulfide indole motility
  16. In SIMS reaction when using cystein you are testing for the presence of
    H2S gas hydrogen sulfide gas will be black
  17. In a SIMS test using cysteine what cases the black precipitate
    Hydrogen sulfide reacting to iron sulfate
  18. In a SIMS test cysteine is a
    Amino acid
  19. In a SIMS reaction using tryptophan and water you are testing for the presents of
    Indole pyruvic acid and ammonia
  20. In a SIMS test using tryptophan when adding a reagent kovacs
    A red ring appeared making test positive for Indole
  21. When observing SIMS Tubes to determine motility
    You look for growth over entire surface and down stab
  22. In a SIMS test is E coli positive or negative
    Positive for hydrogen sulfide, Indole and motolity
  23. In a nitrate test you are testing for a reduction
    Nitrate to nitrite or Nh3 toNh2
  24. In a nitrate test, to test the presents of nitrite you add
    sulfaniliac acid and DAN if the broth turns red nitrite
  25. In the hydrolysis of urea the PH indicator phenol red is used to
    see what the stronger base is
  26. When doing a hydrolysis test on urea you are testing the break down of urea to
    Ammonia and CO2 gas
  27. In the hydrolysis of urea what exoenzyme causes breakdown?
    Urease
  28. In hydrolysis of urea what color is positive and what organism test positive?
    Hot pink 6.9+ and Proteus mirabilis
  29. Fermentation of carbohydrates refers to
    The production of acid and gas
  30. In fermentation is O2 required ?
    No
  31. In fermentation of carbohydrates the ph indicator is
    Phenol Red
  32. In fermentation of carbohydrates the broths contain
    Sucrose, lactose, glucose and or mannitol
  33. The Durham tube is used to
    collect gas
  34. In fermentation of carbohydrates the final end product is
    Acid
  35. In fermentation the final acceptor of____is another organic molecule.
    Electrons
  36. In fermentation of carbohydrates if the broth is red it indicates
    Negative and it is basic
  37. In fermentation of carbohydrates if the broth is yellow it indicates
    Positive acid
  38. In fermentation of carbohydrates the energy source for a week base is
    Amines
  39. In a fermintation test, using Staphyloccus aures, what carbohydrates are positive for fermintation?
    Glucose and Lactose
  40. In a fermintation test, using Alcaligenes faecalis, what carbohydrates are positive for fermintation?
    Lactose
  41. In a fermintation test, using Escherichia Coli, what carbohydrates are positive for fermintation?
    Glucose and lactose
  42. In fermentation of carbohydrate the catalyst is
    Pyruvic acid
  43. In the production of decarboxylase the three amino acids are
    Lysine , Arginine and Ornithine
  44. In the production of Decarboxylase the ph indicator is
    Bromcresol purple
  45. In the production of decarboxylase the catalyst is
    Decarboxylase
  46. In the production of decarboxylase the final end product is
    Carbon dioxide gas
  47. In a decaboxylase production test, using Klebsiella pneumoniae, what amino acids are positive for decarboxylase?
    Lysine
  48. In a decaboxylase production test, using Enterobacter aerogenes, what amino acids are positive for decarboxylase?
    Lysine and Ornithine
  49. In decarboxylase production if the broth turns purple it is
    Positive with the end product ammonia
  50. In decarboxylase production if the broth turns yellow
    It is negative
  51. In MR-VP reactions MR and VP refers to
    • MR =methyl red
    • VP=Voges Proskauer
  52. In MR-VP the sugar that is used is
    Glucose
  53. In MR-VP testing if MR turns red
    It is a strong acid
  54. In MR-VP if the broth turns yellow or orange it indicates
    The absents of a strong acid which would indicate a base
  55. In MR-VP the end product of MR is a
    Mixed acid
  56. In MR-VP the ph indicators for MR is
    Methyl Red
  57. In MR-VP reaction the by product when doing VP is
    AMC (acetylmethyl corbinal)
  58. When doing a MR-VP reaction the VP ph indicators are
    VPA (a naphthol solution) and VPB (hydroxide creatine solution)
  59. In a MR-VP reaction when doing a VP test it is positive for AMC if
    There is presents of a red ring
  60. In a MSA test the ph indicator is
    Phenol red
  61. In a MSA test the agar used contains
    Mannitol, salt and phenol red
  62. In a MSA test the sugar used is
    Mannitol which is an alcohol sugar
  63. In a MSA test the bacteria that grows in agar is
    Staphylococcus
  64. In a MSA test the agar is positive if it
    Turns yellow
  65. In a MSA test the test is negative if
    Agar stays red
  66. MSA means
    Mannitol salt agar
  67. In a coagulase test the media used to detect fibrin is
    Citrated Rabbit Plasma
  68. In a coagulase test the anticoagulant is
    Sodium citrate
  69. In a coagulase test the end product is
    Fibrin clot
  70. In a coagulase test the virulence factor is
    Exoenzyme coagulase it forms a coating around bacteria
  71. In a Simmons citrate test the ph indicator is
    Bromthymol blue
  72. In a Simmons citrate test the carbon source is
    Citrate
  73. In a Simmons test the by product is
    Acetate and oxalacetate
  74. In a Simmons test the test is positive if the agar turns
    Blue
  75. In a positive Simmons test the bacteria produces
    Alkaline
  76. In a Simmons test the media contains
    Ammonium salts, citrate and Bromthymol blue
  77. In a Simmons test the sole source of nitrogen is
    Ammonium salt instead of amino acids
  78. The five characteristics of coliforms are
    Gram negative, rods, facultative, ferment lactose,non endospore producers,
  79. The media used in a coliform test is
    Eosinmethlyn blue
  80. In a coliforms test what bacteria is grown that makes the test positive
    E coli
  81. The detection of catalase produces the end product
    Oxygen
  82. In the detection of catalase the test is positive if
    There is a detection of bubbles
  83. In the detection of catalase the reagent is
    Hydrogen peroxide
  84. The detection of catalase the end product is
    Oxygen
  85. Yellow broth with air in a Durham tube indicates what?
    Fermintation with acid and gas end products
  86. Enzyme that catalyzes removal of carboxylate group (COOH) from amino acid is?
    Decarboxylase
  87. Mueller-Hinton agar is used for what test?
    Kirby Bauer antibiotic suceptibility test
  88. Differential media with the abitlity to hemolyze RBC's is ?
    Blood agar
  89. In a reduction of nitrate test if the color dosen't change it could be for the following reasons.
    The bacteria doesn't reduce nitrate, it did reduce but it reduced again,to ammonia or nitrigen gas.
  90. In a reduction of nitrate test, what bacteria was positive for nitrate reduction?
    Alcaligenes faecalis
  91. In a reduction of nitrate test, what bacteria tested negative?
    Bacillus spizizenii
  92. In a reduction of nitrate test, what is added by a microbiologist to clarify that a nitrate reduction did occur?
    Zinc (an inorganic catalyst that reduces nitrate to nitrite)
  93. In a Hemolysin production test what doese the media consist of?
    Trypic soy containing 5% sheep blood
  94. In a Hemolysin production test what are the three types of hemolysis?
    Alpha, Beta and Gamma
  95. In a Hemolysin production test if there is a green tinge surrounding the colonies of bacteria this will indicate what type of hemolysis?
    Alpha
  96. In a Hemolysin production test if there is clear zones surrounding the colonies of bacteria because hemoglobin was destroyed this will inicate what type of hemolysis?
    Beta
  97. In a Hemolysin production test if the colonies of bacteria do not destroy the agar and it is blood red this is what type of hemolysis?
    Gamma
  98. In a Hemolysin production test what causes the green tinge in alpha hemolysis?
    The red blood cells are broken down by the organisim and the amino acids are used by organisim but leave iron which remains in agar creating a green tinge.
  99. In a Hemolysin production test, examples of organisims that grow are:
    Apha : Steptococcus pneumoniae


    Beta : Streptococcus pyogenes


    Gamma : Entrococcus faecalis
  100. A clear zone apearing around a disk in anti microbial susceptibility test is called?
    Zone of inhibition
  101. Alternative name for anti microbial susceptibility test is?
    Kirby Bauer & disc diffusion method
  102. In fermintation what is the inverted tube called?
    Durham tube
  103. Organism capable of alpha hemolysis is?
    Streptococcus pneumonia
  104. Organisim capable of beta hemolysis is?
    Streptococcus pyogenes
  105. That is the hydrolysis gelitan, for the carbohydrate test consist of?
    Look Up
  106. Organisim capable of gamma hemolysis is?
    Enterococcus faecalis

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