Card Set Information
Micro lab Exam II
Hydrolysis of starch show positive when
Reagent iodine is added and there is no color change. Around streak
Hydrolysis of starch is negative when
Iodine is added and a dark purple color around streak line
In hydrolysis of starch the coenzyme that promotes hydrolysis of starch into sugar is
Starch is a
When doing a hydrolysis test on starch you are looking for
When doing a hydrolysis of starch Bacillus spizizenii has the coenzyme
When doing a hydrolysis of starch E. Coli has a negative or positive coenzyme of amylase
When doing a Hydrolysis of gelatin the coenzyme that promotes the hydrolysis in gelatin and make it liquefied
When doing a hydrolysis on gelatin you are looking for the presence of
Hydrolysis of gelatin is negative when
The gelatin remains solid
Hydrolysis of gelatin is positive when
Gelatin is liquid
In hydrolysis of gelatin bacillus spizizenii has the coenzyme
Gelatin is a
Amino acids are a
SIMS refers to
Sulfide indole motility
In SIMS reaction when using cystein you are testing for the presence of
H2S gas hydrogen sulfide gas will be black
In a SIMS test using cysteine what cases the black precipitate
Hydrogen sulfide reacting to iron sulfate
In a SIMS test cysteine is a
In a SIMS reaction using tryptophan and water you are testing for the presents of
Indole pyruvic acid and ammonia
In a SIMS test using tryptophan when adding a reagent kovacs
A red ring appeared making test positive for Indole
When observing SIMS Tubes to determine motility
You look for growth over entire surface and down stab
In a SIMS test is E coli positive or negative
Positive for hydrogen sulfide, Indole and motolity
In a nitrate test you are testing for a reduction
Nitrate to nitrite or Nh3 toNh2
In a nitrate test, to test the presents of nitrite you add
sulfaniliac acid and DAN if the broth turns red nitrite
In the hydrolysis of urea the PH indicator phenol red is used to
see what the stronger base is
When doing a hydrolysis test on urea you are testing the break down of urea to
Ammonia and CO2 gas
In the hydrolysis of urea what exoenzyme causes breakdown?
In hydrolysis of urea what color is positive and what organism test positive?
Hot pink 6.9+ and Proteus mirabilis
Fermentation of carbohydrates refers to
The production of acid and gas
In fermentation is O2 required ?
In fermentation of carbohydrates the ph indicator is
In fermentation of carbohydrates the broths contain
Sucrose, lactose, glucose and or mannitol
The Durham tube is used to
In fermentation of carbohydrates the final end product is
In fermentation the final acceptor of____is another organic molecule.
In fermentation of carbohydrates if the broth is red it indicates
Negative and it is basic
In fermentation of carbohydrates if the broth is yellow it indicates
In fermentation of carbohydrates the energy source for a week base is
In a fermintation test, using Staphyloccus aures, what carbohydrates are positive for fermintation?
Glucose and Lactose
In a fermintation test, using Alcaligenes faecalis, what carbohydrates are positive for fermintation?
In a fermintation test, using Escherichia Coli, what carbohydrates are positive for fermintation?
Glucose and lactose
In fermentation of carbohydrate the catalyst is
In the production of decarboxylase the three amino acids are
Lysine , Arginine and Ornithine
In the production of Decarboxylase the ph indicator is
In the production of decarboxylase the catalyst is
In the production of decarboxylase the final end product is
Carbon dioxide gas
In a decaboxylase production test, using Klebsiella pneumoniae, what amino acids are positive for decarboxylase?
In a decaboxylase production test, using Enterobacter aerogenes, what amino acids are positive for decarboxylase?
Lysine and Ornithine
In decarboxylase production if the broth turns purple it is
Positive with the end product ammonia
In decarboxylase production if the broth turns yellow
It is negative
In MR-VP reactions MR and VP refers to
MR =methyl red
In MR-VP the sugar that is used is
In MR-VP testing if MR turns red
It is a strong acid
In MR-VP if the broth turns yellow or orange it indicates
The absents of a strong acid which would indicate a base
In MR-VP the end product of MR is a
In MR-VP the ph indicators for MR is
In MR-VP reaction the by product when doing VP is
AMC (acetylmethyl corbinal)
When doing a MR-VP reaction the VP ph indicators are
VPA (a naphthol solution) and VPB (hydroxide creatine solution)
In a MR-VP reaction when doing a VP test it is positive for AMC if
There is presents of a red ring
In a MSA test the ph indicator is
In a MSA test the agar used contains
Mannitol, salt and phenol red
In a MSA test the sugar used is
Mannitol which is an alcohol sugar
In a MSA test the bacteria that grows in agar is
In a MSA test the agar is positive if it
In a MSA test the test is negative if
Agar stays red
Mannitol salt agar
In a coagulase test the media used to detect fibrin is
Citrated Rabbit Plasma
In a coagulase test the anticoagulant is
In a coagulase test the end product is
In a coagulase test the virulence factor is
Exoenzyme coagulase it forms a coating around bacteria
In a Simmons citrate test the ph indicator is
In a Simmons citrate test the carbon source is
In a Simmons test the by product is
Acetate and oxalacetate
In a Simmons test the test is positive if the agar turns
In a positive Simmons test the bacteria produces
In a Simmons test the media contains
Ammonium salts, citrate and Bromthymol blue
In a Simmons test the sole source of nitrogen is
Ammonium salt instead of amino acids
The five characteristics of coliforms are
Gram negative, rods, facultative, ferment lactose,non endospore producers,
The media used in a coliform test is
In a coliforms test what bacteria is grown that makes the test positive
The detection of catalase produces the end product
In the detection of catalase the test is positive if
There is a detection of bubbles
In the detection of catalase the reagent is
The detection of catalase the end product is
Yellow broth with air in a Durham tube indicates what?
Fermintation with acid and gas end products
Enzyme that catalyzes removal of carboxylate group (COOH) from amino acid is?
Mueller-Hinton agar is used for what test?
Kirby Bauer antibiotic suceptibility test
Differential media with the abitlity to hemolyze RBC's is ?
In a reduction of nitrate test if the color dosen't change it could be for the following reasons.
The bacteria doesn't reduce nitrate, it did reduce but it reduced again,to ammonia or nitrigen gas.
In a reduction of nitrate test, what bacteria was positive for nitrate reduction?
In a reduction of nitrate test, what bacteria tested negative?
In a reduction of nitrate test, what is added by a microbiologist to clarify that a nitrate reduction did occur?
Zinc (an inorganic catalyst that reduces nitrate to nitrite)
In a Hemolysin production test what doese the media consist of?
Trypic soy containing 5% sheep blood
In a Hemolysin production test what are the three types of hemolysis?
Alpha, Beta and Gamma
In a Hemolysin production test if there is a green tinge surrounding the colonies of bacteria this will indicate what type of hemolysis?
In a Hemolysin production test if there is clear zones surrounding the colonies of bacteria because hemoglobin was destroyed this will inicate what type of hemolysis?
In a Hemolysin production test if the colonies of bacteria do not destroy the agar and it is blood red this is what type of hemolysis?
In a Hemolysin production test what causes the green tinge in alpha hemolysis?
The red blood cells are broken down by the organisim and the amino acids are used by organisim but leave iron which remains in agar creating a green tinge.
In a Hemolysin production test, examples of organisims that grow are:
A clear zone apearing around a disk in anti microbial susceptibility test is called?
Zone of inhibition
Alternative name for anti microbial susceptibility test is?
Kirby Bauer & disc diffusion method
In fermintation what is the inverted tube called?
Organism capable of alpha hemolysis is?
Organisim capable of beta hemolysis is?
That is the hydrolysis gelitan, for the carbohydrate test consist of?
Organisim capable of gamma hemolysis is?