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Poxviridae
- •Double-stranded DNA, enveloped viruses
- –Orthopoxvirus (vaccinia and smallpox viruses)
- –Molluscipoxvirus
- –Smallpox
- –Molluscum contagiosum
- –Cowpox
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Picornaviridae
- -Single-stranded RNA, + strand, nonenveloped
- -Enterovirus
- -Poliovirus and coxsackievirus
- Rhinovirus
- -Hepatitis A virus
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Caliciviridae
- •Single-stranded RNA, + strand, nonenveloped
- –Hepatitis E virus
- –Norovirus causes gastroenteritis
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Flaviviridae
- Single-stranded RNA, + strand, enveloped
- –Arboviruses can replicate in arthropods; include yellow fever, dengue, SLE, and West Nile viruses
- –Hepatitis C virus
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Coronaviridae
- Single-stranded RNA, + strand, enveloped
- –Upper respiratory infections
- –Coronavirus
- –SARS
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Filoviridae
- Single-stranded RNA, – strand, one RNA strand
- –Filovirus
- –Enveloped, helical viruses
- –Ebola and Marburg viruses
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Paramyxoviridae
- Single-stranded RNA, – strand, one RNA strand
- –Paramyxovirus
- –Morbillivirus
- –Parainfluenza
- –Mumps
- –Newcastle disease (chickens)
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Orthomyxoviridae
- Single-stranded RNA, – strand, multiple RNA strands
- –Envelope spikes can agglutinate RBCs
- –Influenzavirus (influenza viruses A and B)
- –Influenza C virus
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Bunyaviridae
- Single-stranded RNA, – strand, multiple RNA strands
- –Bunyavirus (CE virus)
- –Hantavirus
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Retroviridae
- Single-stranded RNA, 2 RNA strands, produce DNA
- –Use reverse transcriptase to produce DNA from viral genome
- –Lentivirus (HIV)
- –Oncogenic viruses
- •Includes all RNA tumor viruses
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capsid
the protein coat of a virus that surrounds the nucleic acid
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capsomere
a protein sub unit of a viral capsid
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envelope
an outer covering surrounding the capsid of some viruses
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non-envelope
capsids are not covered
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complex viruses
virus with a complicated structure such as a vacteriophage
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helical viruses
long rods, the viruses causes rabies and ebola fever
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polyhedral viruses
has many sides it is also the shape of an icosahedro
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cytopathic effect (CPE)
cell deterioration
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lytic cycle
a mechanism of phage multiplication that results in host cell
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lysogenic cycle
stages in viral development that results in the incorporation of viral DNA into host DNA
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pinocytosis
engulfing of fluid by infolding of the plasma membrane in eukaryotes
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uncoating
seperation of the viral nucleic acid from its protein coat once the virion is enclosed within the vesicle
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budding
the envelop actually develops around the capsid
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oncogenes
gene that can bring about malignant transformation
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oncogenic viruses
virus that is capable of producing tumors, called oncovirus
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prion
an infectious agent consisting of a self-replicating protein, with no detatachable nucleic acids
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Virus
- submicroscopic parasitic filterable agent con of a nucleic acid surrounded by a protein con.
- family names end in viridae
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genomics
the study of genes and their function
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sense strand
positive strand RNA virus
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anti-sense strand
negative strand RNA virus
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double stranded RNA viruses
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provirus
viral DNA that is intergrated into the host cell's DNA
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The virulence of a microbe is often expressed as the ID 50
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The potency of a toxin is often expressed as the LD 50
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biofilms
a microbial community that usually forms as a slimy layer on a surface
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M protein
a heat and acid resistance protein of streptococcal cell walls and fibrils
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Opa
a bacterial outer membrane protein, cells with opa form opaque colonies
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fimbriae
an appendage on a bacterial cell used for attachment
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coagulases
bacterial exzyme that causes blood plasma to clot
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kinases
a bacterial enzyme that breaks down fibrin(blood clots)
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hyaluronidase
an enzyme secreted by certain bacteria that hydrolyzes hyaluronic acid and helps spread micro organisms from their initial site of infections
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collagenase
breaks down collagen
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IgA proteases
enzymes that deestrooy antibodies
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antigenic variation
changes in surface antigens that occur in a microbial population
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invasins
a surface protein produced by salmonella typhimurium and e. coli that rearranges near by actin filaments in the cytoskeleton of a host cell
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siderophores
bacterial iron-binding proteins
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toxigenicity
the capacity of a microorganism to produce a toxin
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toxemia
the presence of toxins in the blood
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exotoxins
a protein toxin released from living, mostly gram positive bacterial cells
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endotoxins
part of the outer portion of the cell wall (lipid A) of most gram neg bacteria; released on destruction of the cell
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mutation
change in the base sequence of dna
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base substitution
a single base at one point in the DNA sequence is replaced with a different base (Most common)
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missense mutation
a substitution of an amino acid in the synthesized protein
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nonsense mutation
a base substitution in DNA that results in a nonsense codon
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frameshift mutation
a mutation caused by the addition or deletion of one or more bases in DNA
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spontaneous mutation
a mutation that occurs without a mutagen
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mutagen
an agent in the environment that brings about mutations
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chemical mutagens
changes the DNA with exposure to nitrous acid can convert the base adenine to a form that doesn't pair with thtymine
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nucleoside analog
chemical that is structurally similar to the normal nucleosides in nucleic acids but with altered base-pairing properties
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photolyases
an enzyme that splits thymine dimersin the presence of visible light
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nucleotide excision repair
the repairing of DNA involving removal of defectiive nucleotides and replacement with functional ones
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methylases
enzymes add to methyl group to selected bases soon after a DNA strad is made
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bacteriophage
a virus that infects bacterial cells
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saxitoxin
a neurotoxin produced by some dinoflagellates
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mutation rate
the probability a gene will mutate each time a cell divides
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monomers, dimers, polymers
- sugars are calld saccharides
- -mono, di, oligo, poly
- lipids
- -simple; triglyceride
- -complex;phospholipid
- proteins
- -protein structure (folding)
- -peptide bond
- -lock & key model of enzyme fx
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glycocalyx
- outside cell wall
- usually sticky
- capsule; neatly organized
- slime layer; unorganized and loose
- extracellular polysaccaride allows cell to attatch
- capsules prevent phagocytosis
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xanthomonas campestris xanthan gum
polymer ofo glucose, mannose, and glucuronic acid
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organic compounds
- carb (sugars)
- lipids (fat)
- proteins
- nucleic acid (DNA/RNA)
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flagella
- outside cell wall
- made of chains of flagellin protein
- attach to a protein hook
- anchored to the wall and membrane by the basal body
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motile cells
- rotate flagella to run or tumble
- move toward or away from stimula (taxis)
- flagella proteins are H antigens
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Fimbriae and Pili
- Fimbriae allow attachment
- Pili
- -facilitate transfer of DNA from 1 cell to another
- -gliding motility
- -twiching motility
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gram positive cell wall
- thick peptiodoglycan
- teichoic acids
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gram negative cell wall
- thin peptidoglycan
- outer membrane
- periplasmic space
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the cell wall
- prevents osmotic lysis
- made of peptiodoglycan (in bacteria)
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peptidoglycan
- polymer of disaccharide
- -NAG
- -NAM
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plasma membrane
- phospholipid bilayer
- peripheral proteins
- intergral proteins
- transmembrane
- proteins
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fluid mosaic model
- membrane is a viscous as olive oil
- proteins move to fx
- phospholipids rotate and move laterally
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gram-negative outer mebrane
- protection from phagocytes, complement, and antibiotics
- o polysaccharide antigen
- lipid A is an endotoxin
- porins (proteins) form channels through membrane
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simple diffusion
movement of a solute from an area of high concentration to an area of low concentration
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facilitated diffusion
solute combines with a transporter protein in the membrane
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osmosis
the movement of water across a selectively permeable membrane from an area of high water to an area of lower water concentration
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osmotic pressure
the pressure needed to stop the movement of water across the membrane
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cytoplasm
the substance inside the plasma membrane
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prokaryotic ribosome
- protein synthesis
- 70s
- 50s + 30s subunits
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endospores
- resting cells
- resistant to desiccation, heat, chemicals
- bacillus, clostridium
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sporulation
endospore formation
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germination
return to vegetative state
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cell wall and glycocalyx
- cell wall
- -plants, algae, fungi
- -carbs
- cellulose, chitin, glucan, mannan
- glycocalyx
- -carbs extending from animal plasma membrane
- -bonded to proteins and lipids in membrane
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plasma membrane
- selective permability allows passage of some molecules
- simple diffusion
- facilitative diffusion
- osmosis
- active transport
- endocytosis
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phagocytosis
pseudopods extend and engulf particles
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pinoocytosis
membrane folds inward bringing in fluid and dissolved substances
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cytoplasm
cytoplasm membrane-substance inside plasma and outside the nucleus
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cytosol
fluid portion of cytoplasm
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cytoskeleton
microfilaments, intermediate filaments, microtubules
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cytoplasmic streaming
moveement of cytoplasm throughout cell
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organelles
- nucleus- contains chromosomes
- er- transport network
- golgi complex- membrane formation and secretion
- lysosome- difestive enzymes
- vacuole- brings food into cells and provides support
- mitochondrion- cellular respiration
- chloroplast- photosynthesis
- peroxisome- oxidation of fatty acides; destroys h202
- centrosome- consists of protein fibers and centrioles
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