lab exam 2

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Anonymous
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153486
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lab exam 2
Updated:
2012-05-11 20:32:43
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microbiology
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microbiology
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  1. gram positive
    rod
    obligate aerobe
    acid fast
    mycobacterium smegmatis
  2. gram positive
    rod
    faculative anaerobe
    lactobacillus casei
  3. gram positive
    rod
    obligate anaerobe
    endospores
    clostridium sporogenes
  4. gram positive
    rod
    faculative anaerobe
    endospores
    catalase
    bacillus cereus
  5. gram negative
    rod
    indole
    obligate aerobe
    urease
    little/ no fermentation- red TSI
    pseudomonas aeruginosa
  6. gram negative
    rods
    sulfur, indole, motility
    faculative anaerobe
    urease
    hydrogen sulfide- black TSI
    proteus hauseri
  7. gram negative
    rods
    green/metallic- EMB
    indole, motility
    faculative anaerobe
    MR
    gas and sugar metabolised-yellow TSI
    escherichia coli
  8. gram negative
    rods
    pink- EMB
    motility
    faculative anaerobe
    MR
    urease
    gas and sugar metabolized- yellow TSI
    klebsiella pneumonia
  9. gram negative
    rods
    pink- EMB
    faculative anaerobe
    VP
    gas and sugar metabolized- yellow TSI
    enterobacter aerogenes
  10. growth on EMB indicates bacteria is
    gram negative
  11. pink growth on EMB
    klebsiella pneumoniae, enterobacter aerogenes
  12. SIM
    • sulfur- black
    • indole- red at surface of media
    • motility- widespread growth
  13. interpreting O/F glucose
    • both tubes yellow= O + F=faculative anaerobe
    • mineral oil tube green= O only= obligate aerobe
    • mineral oil tube yellow= obligate anaerobe
  14. interpreting urease
    • light pink- weak positive
    • bright pink- positive

    for enzyme urease
  15. interpreting TSI
    • yellow= glucose, lactose and sucrose fermentation
    • red= only glucose
    • gaps/cracks= gas production
    • black= sulfur production
  16. interpreting MR-VP
    • VP= pink or red is positive
    • MR=immediate red color change is positive
  17. staph aureus disinfectant- best and worst
    • best= hydrogen peroxide
    • worst= mouthwash
  18. bacillus cereus disinfectants- best and worst
    • best= hydrogen peroxide
    • worst= alcohol
  19. enterococcus faecalis disinfectants- best and worst
    • best= hydrogen peroxide
    • worst= mouthwash
  20. pseudomonas aeruginosa disinfectants- best and worst
    • best= hydrogen peroxide
    • worst= mouthwash
  21. staph aureus resistant antibiotics
    • aminoglycosides
    • chloramphenicol
  22. staph aureus sensitive antibiotics
    • tetracycline
    • penicillin
  23. bacillus cereus resistant antibiotics
    • tetracycline
    • penicillin
  24. bacillus cereus sensitive antibiotics
    aminoglycosides
  25. enterococcus faecalis resistant antibiotics
    aminoglycosides
  26. enterococcus faecalis sensitive antibiotics
    penicillin
  27. pseudomonas aeruginosa resistant antibiotics
    • chloramphenical
    • tetracycline
    • penicillin
    • aminoglycosides
  28. kovac's reagent added to
    SIM

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