Microbiology

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Author:
jessem30
ID:
154504
Filename:
Microbiology
Updated:
2012-05-17 22:56:30
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Microbiology lab test
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Description:
microbiology lab test
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  1. Why do we use a simple stain?
    To determine the shape of bacteria.
  2. The four stains used in a gram stain
    • Crystal Violet
    • grams iodine
    • Ethyl Alcohol
    • sarafin
  3. What color does the Crystal Violet stain in a gram stain
    Purple
  4. What color does the Grams Iodine stain in a gram stain?
    Purple
  5. What color does the ethyl alcohol stain in the gram stain?
    Clear
  6. What color does the sarfarin stain in the gram stain?
    Pink
  7. If the gram stain is positive what color will you see?
    Purple
  8. If the gram stain is negative what color will you see?
    Pink
  9. How long do you stain crystal violet in a gram stain and what color will it be?
    20 secs/Purple
  10. How long will you stain grams iodine in a gram stain and what color will it be?
    1 min/purple
  11. How long do you stain sarfarin on a gram stain and what color?
    1min/Pink
  12. What are the three stains used in an acid fast stain?
    • Carbolfuchin
    • Acid alcohol
    • methylene blue
  13. If the stain is acid fast what color will it be?
    Pink
  14. If the stain is non acid fast what color will it be?
    Blue
  15. In an acid fast stain what color does the carbolfuchain stain and how long do you leave it on?
    Pink/ 5-6 min
  16. In an acid fast stain what color does methyl blue stain and how long do you leave it on?
    Blue/2 min
  17. Do you boil water for and acid fast stain?
    Yes
  18. What stain do you use for negative stain?
    nigrosin
  19. At what angle do you smear a negative stain?
    45 degrees
  20. do you air dry a negative stain?
    Yes
  21. What does a negative stain look like?
    a picture negative.
  22. What stains do you use for a capsule stain?
    • Nigrosin
    • Crystal violet or safranin
  23. where do you stain a capsule stain?
    over a staining rack or tray or sink.
  24. What stains do you use for a endospore stain?
    • Malachite green
    • Safranin
  25. In an endospore stain how long do you leave the malachite green on and what color does it stain?
    5-6 min/ Green
  26. In an endospore stain how long do you leave on the safranin and what color will it stain?
    60-90 secs/ pink
  27. When the safranins is added as a counter stain and the cell has endospores what will it look like?
    Pink with a green middle.
  28. When safranins is added as a counter stain what do cells without and endospore look like?
    Just pink
  29. Three types of Media?
    • Agar
    • Broths
    • Gelatins
  30. What is a Broth?
    A liquid media
  31. What is a gelatin
    A broth with gelatin added
  32. How much extra media do you make?
    20% (multiply X 1.2)
  33. What is the minimum of media you can make?
    50ml
  34. The final weight measurement of the powder should be calculated to the nearest _____ of a gram
    tenth/ 2.73=2.7g
  35. Fluid will be rounded up to the nearest?
    the next highest tenths place. 73ml=80ml
  36. Small tubes require __ml
    5ml
  37. Large tubes require ___ml
    10ml
  38. Petri plates require ___ml
    25ml
  39. three shapes of bacteria?
    • Cocci
    • Bacilli
    • sprial shapes
  40. Shape of Cocci
    Round
  41. tetrad cocci?
    4 cocci buched together
  42. Streptococci
    Cocci linked in a chain
  43. Staphylococci
    more than 4 cocci bunched together
  44. Occular lens
  45. Nosepiece
  46. oil immersion objective lens
  47. Slide holder
  48. Stage
  49. Diaphragm lever
  50. Condenser
  51. Light source
  52. Base
  53. Power switch
  54. Rheostat
  55. Course focus adjustment knob
  56. fine adjustment knob
  57. Mechanical stage control

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