Card Set Information
Microbiology lab test
microbiology lab test
Why do we use a simple stain?
To determine the shape of bacteria.
The four stains used in a gram stain
What color does the Crystal Violet stain in a gram stain
What color does the Grams Iodine stain in a gram stain?
What color does the ethyl alcohol stain in the gram stain?
What color does the sarfarin stain in the gram stain?
If the gram stain is positive what color will you see?
If the gram stain is negative what color will you see?
How long do you stain crystal violet in a gram stain and what color will it be?
How long will you stain grams iodine in a gram stain and what color will it be?
How long do you stain sarfarin on a gram stain and what color?
What are the three stains used in an acid fast stain?
If the stain is acid fast what color will it be?
If the stain is non acid fast what color will it be?
In an acid fast stain what color does the carbolfuchain stain and how long do you leave it on?
Pink/ 5-6 min
In an acid fast stain what color does methyl blue stain and how long do you leave it on?
Do you boil water for and acid fast stain?
What stain do you use for negative stain?
At what angle do you smear a negative stain?
do you air dry a negative stain?
What does a negative stain look like?
a picture negative.
What stains do you use for a capsule stain?
Crystal violet or safranin
where do you stain a capsule stain?
over a staining rack or tray or sink.
What stains do you use for a endospore stain?
In an endospore stain how long do you leave the malachite green on and what color does it stain?
5-6 min/ Green
In an endospore stain how long do you leave on the safranin and what color will it stain?
60-90 secs/ pink
When the safranins is added as a counter stain and the cell has endospores what will it look like?
Pink with a green middle.
When safranins is added as a counter stain what do cells without and endospore look like?
Three types of Media?
What is a Broth?
A liquid media
What is a gelatin
A broth with gelatin added
How much extra media do you make?
20% (multiply X 1.2)
What is the minimum of media you can make?
The final weight measurement of the powder should be calculated to the nearest _____ of a gram
Fluid will be rounded up to the nearest?
the next highest tenths place. 73ml=80ml
Small tubes require __ml
Large tubes require ___ml
Petri plates require ___ml
three shapes of bacteria?
Shape of Cocci
4 cocci buched together
Cocci linked in a chain
more than 4 cocci bunched together
oil immersion objective lens
Course focus adjustment knob
fine adjustment knob
Mechanical stage control