Clin Chem Prac

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Author:
lynnsy
ID:
155793
Filename:
Clin Chem Prac
Updated:
2012-08-23 00:36:23
Tags:
Clin Chem
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Description:
alittle review
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    • Griffin Beaker
    • used for holding and missing solutions
    • Erlenmeyer Flask
    • used for holding and mixing solutions
    • Volumetric Flask
    • measures one volume only
  1. Graduated Cylinder
    • Cuvette
    • measure in spectrophotometry
    • Pasteur Pipette
    • Not for measuring
    • Plastic transfer pipette
    • not used for measuring
    • Serological Pipette
    • Fairly accurate for measuring
    • Volumetric Pipette
    • most accurate for measuring, one volume
    • TC = to contain
    • TD = To deliver
    • Automatic Pipette
    • measures one volume
    • 1000 microunits = 1ml
  2. Centrifuge speeds and times
    • Blood = 6000rpm/5min
    • Urine = 1500rpm/3-5mins
    • Feces = 1200rpm/5-10mins
  3. Functional Unit of Kidney
    • Nephron
    • Fultration / reabsorption
  4. Freely filtrated substances
    • water
    • electrolytes
    • glucose
    • Amino acids
    • Urea and Creatine
  5. Urea comes from
    Protein breakdown > ammonia > urea > urine
  6. Creatinine comes from
    muscle cell metabolism > creatinine > urine
  7. Hormones that acts on renal tubules
    • ADH > reabsorb water
    • Aldosterone > controls Na reabsorp and K excretion
  8. Glucose normals and threshold
    • 100mg/dl
    • 160-180mg/dl
  9. 4 methods of collection
    • Free catch
    • Manual Expression
    • Cathiterization
    • Cystocentesis 22ga 1-1 1/2 inch
  10. Urine samples storage
    • 30min room temp
    • 6hr fridge
    • 1 drop of 40% formaldehyde to 1oz urine
  11. 4 parts to routine U/A
    • Macroscopic eval
    • USG
    • Urine Chems
    • microscopic Eval
  12. USG evals...
    • Renal tubular function
    • how well responds to ADH
    • reabsorbs water into bloodstream
  13. Fixed USG
    • 1.008-1.012
    • 2/3 - 3/4 nephrons are non functional
  14. BUN and Creat Normals
    • BUN
    • Dog - 7-27mg/dl
    • Cat - 16-36mg/dl
    • Creat
    • Dog - 0.5-1.8mg/dl
    • Cat - 0.8-2.4mg/dl
  15. Azotemias
    • Pre - ^BUN ^Creat ^USG
    • Primary - ^ ^ fixed
    • Post - ^ ^ ^ PCV ^ TP^ = pre
  16. Liver Dz
    ALT
    AST
    D.Bili
    ALP
    GGT
    ALB
    GLOB
    BUN
    • U ALT
    • U AST
    • U D.Bili
    • U ALP
    • U GGT
    • D ALB
    • D GLOB
    • D BUN
  17. Dehydration
    ALB
    GLOB
    Na
    Cl
    K
    USG
    • u ALB
    • u GLOB
    • u Na
    • u Cl
    • u K
    • u USG
  18. Blocked Tom
    BUN
    Creat
    K
    Na
    phos
    USG
    • u BUN
    • u Creat
    • u K
    • u Na
    • u phos
    • u USG
  19. Diuresis
    BUN
    Creat
    K
    Na
    USG
    • d BUN
    • d Creat
    • d K
    • d Na
    • d USG
  20. Vomiting
    Na
    K
    Cl
    HCO3
    • d Na
    • d K
    • d Cl
    • u HCO3
  21. Anorexia
    CHOL
    ALB
    BUN
    GLU
    GLOB
    • d CHOL
    • d ALB
    • d BUN
    • d GLU
    • d GLOB
  22. Hemolysis
    AST
    I.Bili
    K
    Phos
    • u AST
    • u I.Bili
    • u K
    • u Phos
  23. Liver Failure
    ALB
    GLOB
    BUN
    CHOL
    • d ALB
    • d GLOB
    • d BUN
    • d CHOL
  24. Pancreatitis
    Amylase
    Lipase
    • u Amylase
    • u Lipase
  25. Metabolic Acidosis
    pH
    TCO2
    Resp Rate whan Compensating
    • d pH
    • d TCO2
    • u Resp Rate whan Compensating
  26. HBO
    ALT
    AST
    Alk. Phos.
    D.Bili
    CHOL
    • u ALT
    • u AST
    • u Alk. Phos.
    • u D.Bili
    • u CHOL
  27. Renal Failure
    BUN
    Creat
    USG
    • u BUN
    • u Creat
    • d USG
  28. LSA
    Ca
    u Ca
  29. Parathyroidectomy
    Ca
    phos
    • d Ca
    • d phos
  30. Addison's Dz
    Na
    K
    Heartrate
    USG
    • d Na
    • u K
    • d Heartrate
    • d USG
  31. Metabolic Alkalosis
    pH
    TCO2
    Resp. Rate when compensating
    • u pH
    • u TCO2
    • d Resp. Rate when compensating
  32. Diarrhea
    Na
    K
    Cl
    HCO3
    • d Na
    • d K
    • u Cl
    • d HCO3
  33. Grass Tetany
    Mg
    d Mg
  34. CO2 + H2O >><< H2CO3 >><< H + HCO3
    Resp -------------------------------- Metabolic
    • pCO2
    • TCO2 = HCO3
  35. Resp. Acidosis
    pH
    pCO2
    Resp. Rate comp
    • d pH
    • u pCO2
  36. Resp. Alkalosis
    pH
    pCO2
    • u pH
    • d pCO2
  37. azotemia
    an increase in non-protein nitrogenous wastes in blood (BUN/creatinine)
  38. pre renal azotemia
    • problem is before the kidneys
    • Inc: BUN, Creat amd USG PCV, TP
    • V/D
  39. primary renal azotemia
    • problem is the kidney itself
    • Inc: BUN, CREAT
    • Fixed usg: 1.008-1.012, 1.015 in cats
  40. Post renal azotemia
    Inc: BUN, CREAT, USG

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