Lab Practical 2 - Sheet1.csv

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dromero337
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156994
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Lab Practical 2 - Sheet1.csv
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2012-06-03 03:27:13
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Lab pratical
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Enterbacteraea and campylobacter
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  1. Enterobacteriacae general info
    Gram negative rods. Oxidase negative. Catalase positive. Facultative anaerobes. Ferment glucose. Usually cause GI diseases. Main difference is the ability to ferment Lacotose or Non lactose fermenting. Lac positive are usually normal flora and cause opportunistic infections nosocomial. Where as lac negative are normally outright pathogens.
  2. Esherichia coli O157:H7 fermentation
    Cannot ferment sorbital. Main difference between normal Escherichia coli. this can be distinguished by a SMAC plate. If the E.coli does not ferment the sorbital in the plate it is O156H7
  3. Brilliant green agar (BGA)
    Isolation of most Salmonella species. Selective agents. Brilliant green inhibits growth of Gram positives and most Gram negatives highly selective for Salmonella. DIfferential agents Lactose and sucrose bacteria that ferment these sugars produce yellowish colonies with yellow halo. Salmonella spp. do not ferment lac or suc colonies appear white with red halo
  4. Eosin-methylene blue (EMB) agar
    "Primary plating medium for Gram negative enterics. Selective agents Eosin & methylene blue dyes: select for growth of Gram negatives
  5. Hektoen-enteric (HE) agar
    "Primary plating medium for Gram negative enterics. Selective agents Bile salts inhibit growth of many Gram positives and non-enteric Gram negatives. Selective agents Sugars (lactose sucrose salicin): bacteria that ferment 1 or more of these sugars produce yellow/orange colonies
  6. H2S production: bacteria that produce H2S form colonies with black centers. Often used for isolation of Salmonella and Shigella species H2S production is the differentiating factor
  7. "
  8. MacConkey agar (MAC)
    Primary plating medium for Gram negative enterics. Selective agents Bile salts and crystal violet inhibit growth of most Gram positives. Differential agents. Lactose: bacteria that ferment lactose produce bright pink colonies. E. coli (and some other enterics) precipitate bile salts and produce cloudy zones around the colonies
  9. SMAC (MAC + sorbitol) agar
    Isolation and identification of E. coli O157H7. Exactly same as MAC plate but has Sorbitol bacteria that ferment sorbitol produce bright pink colonies. Since O157H7 can not ferment this it will have buff colored colonies.
  10. Salmonella-Shigella (SS) agar
    "Isolation and identification of Salmonella and Shigella species. Selective agents Biles salts and various dyes inhibit growth of most Gram positives. Differential agents Lactose bacteria that ferment lactose produce reddish colonies
  11. H2S production bacteria that produce H2S form colonies with black centers. Salmonella and Shigella will form colorless colonies Salmonella is positive for H2S
  12. "
  13. Triple sugar iron (TSI) agar
    "Glucose lactose and sucrose fermentation pattern Ferrous iron forms black precipitate if H2S is produced. Yellow butt red slant only glucose fermented
  14. Yellow butt yellow slant glucose and lactose OR sucrose fermented
  15. "
  16. Xylose-lysine-deoxycholate (XLD) agar
    "Primary plating medium for Gram negative enterics especially useful for Shigella isolation/identification. Selective agent Deoxycholate (bile) inhibits most Gram positives. Selective agents Xylose bacteria that ferment xylose produce yellow coloniesH2S production bacteria that produce H2S form colonies with black centers. Shigella does not ferment xylose and appears red Salmonella ferments xylose and appears yellow
  17. After prolonged incubation Salmonella will decarboxylate lysine and turn red can then be distinguished from Shigella by black center
  18. "
  19. LOAC decarboxylase
    Used primarily for Lac negative Enterobacteriacae. Are the sugars Lysine. Orithine. Arnthenine. and a Control. Must use mineral oil on top to create anaerobic environment for fermentation. Works by removing carboxylic acids and can use them for an energy source making the solution basic. The tubes contain glucose plus one of the amino acids. If the bacteria can use the AA the solution will turn basic by breaking down the AA to an amine. The solution will turn back to its original blue color. yellow is a negative test.
  20. Phenylalanine deaminase
    Is an enzyme that removes an amine from phenylalanine. Uses teh enzyme deaminase to create phenyl pyruvic acid and an amine. To test this add ferric choloride to the slant and look for a BRIGHT GREEN COLOR.
  21. Eschericia coli metabolic results
    TSI A/AG-. h2s negative. IDOLE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. vp negative. citrate negative. urease negative. maltose negative.
  22. Eschericia coli 0157h7 metaloic results.
    TSI A/AG-. h2s negative. IDOLE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. vp negative. citrate negative. urease positive. maltose negative. Main test SMAC PLATE WITH DULL COLORED COLINES. Not orange like normal e. coli.
  23. Citrobacter frundii
    A/AG -. ((H2S POSITIVE)). Indole negative. MOTILITY POSITVE. . MR POSITIVE. vp negative. ((CITRATE POSITIVE)). . UREASE POSITVE. ((MALONATE NEGATIVE)).
  24. Citrobacter diversus
    A/AG-. h2s negative. INDOLE POSITIVE. Motility positive. MR POSITIVE. . vp negative. CITRATE POSITIVE. UREASE POSITVE ((MALONATE POSITIVE). Between fruendi and diverusus use malonate and h2s and indole.
  25. Enterobacter aerogenes
    A/AG -. h2s negative. indole negative. MOTILITY POSITVE. mr negative. VP POSITVE. CITRATE POSITVE. urease negative. MALONATEPOSITIVE. VERY MOTILE!!!!
  26. Klebsiella pneumonia
    A/AG-. h2s negative. Indole negative. motility negative. mr negative. VP POSITIVE. UREASE POSITVE. CITRATE POSITVE. MALONATE POSITVE. to differentiate between citrobacter and klebisella use molilty and that citrobacter is MR positve. Remeber k. pneumonia is different from k. oxytoca by indole
  27. Klebsiella oxytoca
    A/AG-. h2s negative. ((INDOLE POSITIVE)). motility negative. mr negative. VP POSITIVE. UREASE POSITVE. CITRATE POSITVE. MALONATE POSITVE. to differentiate between citrobacter and klebisella use molilty and that citrobacter is MR positve. Remeber k. pneumonia is different from k. oxytoca by indole
  28. Enterobacteriaea lac positive general names
    Eserichia coli. Citrobacter deversus. Citrobacter freundii. Enterobacter areogenes. Klebisella pneumonia. Klebisella oxytoca.
  29. Proteus mirabilis
    SWARMER. K/AG+.(( H2S NEGATIVE)) SULFIDE POSITIVE. MOTILITY POSITVE. MR POSITIVE. vp negative. CITRATE POSITVE. UREASE POSITVE. PHEN. DEAMINASE POSITVE. Lysine decarb negative. arginine decarb negative. Ornithine decarb. Sucrose positve
  30. Proteus vulgaris
    SWARMER (((((A/A+))))). INDOLE POSITIVE. SULFIDE POSITIVE. MOLTILITY POSITIVE. MR POSITIVE. citrate negative. UREASE POSITIVE. . PHEN DEAMINASE POSITVE. Lysine decarb. Arginine decarb negative. Orithine negative. SUCROSE POSITIVE.
  31. Proteus stuartii
    K/A-. INDOLE POSITIVE. (((SULFIDE NEGATIVE)))). MOTILITY POSITIVE. MR POSITIVE. vp negative. CITRATE POSITIVE. UREASE NEGATIVE. PHENYLDECARB POSITVE. lysine. ariginine ornithine negative. sucrose negative
  32. How to differentiate between the Proteus
    PROTEUS IS A SWARMER AND HIGHLY MOTILE. ALL PHEN. DEAMINASE POSITIVE. MOTILITY POSITIVE. Proteus stuartii is the only H2S NEGATIVE. Proteus mirabolis is K/AG+ and indole and sucrose negative but citrate postive ornithine Positve. Proteus vulgaris is A/Ag+ and ferments sucrose. Is indole negative and cirtrate negative. ornithine negative.
  33. Shigella flexneri
    K/AG-. indole negative. sulfide negative. motility negative. MR POSITVE. vp negative.urease negative. Phen. deaminase negative. lysine decarb. negative. ARGININE DECARB POSITVE. Orithine negative. Sucrose negative
  34. Shigella sonnei
    K/AG-. indole negative. sulfide negative. motility negative. MR POSITVE. vp negative.urease negative. Phen. deaminase negative. lysine decarb. negative. arginine decarb negative.. ORINTHINE POSITVE. Sucrose negative
  35. Salmonella typhimurium
    K/AG+. Indole negative. SUFLIDE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. CITRATE POSITIVE. vp negative. Phenyl deaminase negative. LYSINE POSITVE ARGININE POSITIVE. ORNITHINE POSITVE. Sucrose negative
  36. Yersinia enterocolitica
    A/A- INDOLE POSITIVE. Sulfide negative. MOTILITY POSITIVE. MR POSITIVE. VP negative. Citrate negative. UREASE POSITIVE. Phen. deaminase negative. Lysine decarb negative. Arginine decarb negative. ORNITHINE POSITIVE. SUCROSE POSITIVE. IS NON MOTILE AT 35 DEGREE BUT MOTILE AT ROOM TEMP.
  37. Split up lac negative enterbactericea
    If it is PHEN. DEAMINASE POSITVE AND MOTILE (SWARMING) PROTEUS.. Negative everything except MR or One decarb AA is SHIGELLA. ALL POSITVE DECARBS IS SALMONELLA TYPHIMURIUM. If it is H2S negative and Indole positve and phen. negative but sucrose postive is Yersina enterocolitica.
  38. Differentiate between Protues
    Look for H2S production. Non H2S is Y. stuartii. Only H2S positive with A/AG+ is P. vulgaris and this is because of SUCROSE. P. mirabilis is positive for citrate and negative indole and positive ornithine.
  39. Campylobacter general information
    Gram negative s shaped. can be helical. OXIDASE POSITIVE. Microaerohilic. When staining it is hard to do secondary stain and must use carbofusion instead of safranin.
  40. Campy-CVA agar
    Isolation and growth of Campylobacter especially C. jejuni. Selective agents Antibiotics (cefoperazone vancomycin amphotericin B) inhibit growth of other bacteria. Contains sheep blood
  41. Charcoal-based selective media (CSM)
    Isolation and growth of Campylocbacter species. Antibiotics (cefoperazone vancomycin cyclohexamide) inhibit growth of other bacteria. Blood-free medium charcoal acts as a quencher of reactive O2 species
  42. Marine agar (MAR)
    Isolation and enumeration of marine bacteria. selective High salt concentration: selects for halophilic bacteria. No differential agent Vibrio cholerae is not halophilic and will not grow! All other Vibrio species should grow
  43. Rimler-Shotts (RS) media
    "Isolation and identification of Aeromonas species. Maltose colonies that ferment maltose appear yellow
  44. Thiosulfate detection of H2S production. Contains novobiocin
  45. Lysine and ornithine bacteria that decarboxylate lysine or ornithine turn media yellow.
  46. "
  47. Thiosulfate-citrate-bile salts-sucrose (TCBS) agar
    "Primary isolation of Vibrio species particularly V. cholerae. Bile salts inhibit Gram positive organisms
  48. Alkaline pH enhances growth of Vibrio and discourages growth of other enterics. Thiosulfate and ferric ammonium citrate detection of H2S production Sucrose colonies that ferment sucrose appear yellow. Good for differentiating Vibrio species based on sucrose fermentation
  49. "
  50. Campylobacter jejuni
    NALIDIXIC ACID SENSITIVE. (disk placed on campy CVA agar) cephalothin resistant (disk on campy CVA). GROWTH AT 42 POSITIVE. HIPPURATE POSITIVE.
  51. C.fetus
    Nalidixic acid negative. CEPHALOTHIN SENSITIVE. Growth at 42 negative. hippurate negative

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